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19 indicating that lignin rich in S-units will easily degrade even under mild alkaline conditions.A newer application of data envelopment analysis (DEA) model with robust data envelopment analysis (RDEA) was presented for optimization of reaction variables of graft copolymerization of 2-hydroxyethyl methacrylate (HEMA) to Tamarindus indica seed polysaccharide (TSP). It helped to find out the most appropriate reaction conditions and variables (concentrations of HEMA and reaction initiator; temperature and time duration) for copolymerization. The data generated through the experimental work has been analyzed and indexed to predict the maximum %grafting. Sensitivity analysis was performed to check robustness of efficiency scores of CCR DEA efficient samples and a comparative analysis of the CCR DEA and RRDEA efficiency score has been done. The data obtained via real-time experiments and data predicted using computational modelling predictions were found to be in close vicinity.Lignin is an important renewable energy source as an excellent new battery fuel and ideal substitutes for the petrochemical industry. However, the molecular mechanism underlying lignin biosynthesis in wood formation of P. Silmitasertib massoniana remains unexplored. Thus, an integrative analysis of wood biomass and the developing xylem transcriptome was performed to identify genes involved in lignin biosynthesis. A total of 1624 differentially expressed genes (DEGs) were identified, consisting of 797 upregulated and 827 downregulated genes (MaxG vs MinG). Additionally, 122 candidate genes and 17 DEGs were successfully annotated to the lignin biosynthesis pathway. All upregulated MYB and NAC genes were regulators of secondary cell wall formation. Moreover, the qRT-PCR analyses shown that 9 lignin biosynthesis-related genes and 7 transcription factor-encoding genes were upregulated (MaxG vs MinG), which indicated that the downregulation of lignin biosynthesis-related genes might be the possible causes of growth retardation and dwarf phenotype in some P. massoniana individuals. The identification of lignin biosynthesis-related genes can provide valuable genetic basis and resource for further researches on molecular mechanisms of lignin biosynthesis and contribute to the future investigations of bioengineering and synthetic biology to regulate lignin content in wood formation for the pulp and wood utilization industry.Uncontrolled bleeding has always been a sudden accident, which is the main cause of casualties in war trauma, emergency events and surgical operations. Rapid hemostatic materials can effectively reduce casualties and save lives. In this paper, marine collagen peptide grafted carboxymethyl chitosan (CMCS-MCP) was synthesized by 1-ethyl-(dimethylaminopropyl) carbodiimide (EDC)-mediated coupling reaction. To obtain CMCS-MCP conjugates with different degrees of substitution (DS), the reaction conditions were investigated by single-factor tests and optimized by response surface methodology. And the sponges of CMCS-MCP were prepared by freeze-thaw cycling and freeze-drying and characterized by Fourier transform infrared (FTIR) spectroscopy, scanning electron microscope (SEM), and X-ray diffraction (XRD). To evaluate the hemostatic properties of CMCS-MCP sponges, in vitro and in vivo hemostasis tests were carried out. The results showed that the optimum preparation conditions were the mass ratio of MCP to CMCS (MMCP/MCMCS) 61, reaction temperature 41 °C, and reaction time 16 h. And under which the DS of 58.86% was obtained. Structure analysis showed that MCP had been successfully grafted onto the CMCS molecular chain, and the CMCS-MCP sponges were of high porosity. In vitro and in vivo hemostasis tests showed that the CMCS-MCP sponges had significant procoagulant activities, especially the one with high DS of 58.86%. The hemostasis mechanism may be that the synergistic effects of MCP and CMCS accelerated coagulation through multiple approaches. The CMCS-MCP sponges give a new insight into biomedical hemostasis materials.Chronic hyperglycemia results in the formation of advanced glycation end-products (AGEs) and triggers amyloid fibril formation. Molecules designed to inhibit amyloid fibrils function by eliminating toxic oligomers or reducing fibril formation. Here, the bioactivity of polyphenols in redirecting the self-assembly of amyloid fibrils was reported through microscopic, spectroscopic and molecular docking studies. Our findings illustrate that glycation causes BSA to self-assemble into amyloid fibrils. 17 Lys residues had modified to carboxy methyl lysine (CML) but only Lys523 was probable of modifying into carboxy ethyl lysine (CEL). In contrast, only 6 Arg residues are identified to be modified to Argpyrimidine (Arg-p). A simple polyphenol baicalein (BLN) redirect the self-assembly of amyloid fibrils into off-pathway hybrid nanostructures. Circular dichroism spectroscopic studies suggested that in the presence of BLN helical conformation was favored. Molecular modeling studies suggested that hydrogen bonding and hydrophobic interaction of polyphenols preferentially at crucial amyloidogenic regions can hinder amyloid fibrillation (Phe133, Lys136, Tyr137, Ile141, Tyr160 and Arg185). Mass spectrometric results illustrated that the presence of a simple polyphenol BLN several residues are unmodified to CML, CEL or Arg-p. Together, our findings suggest that polyphenols could have a protective effect and the redirection can help alleviate the amyloid fibril formation.We have analyzed an effect of imidazolium cation-Hofmeister anion salts on stability of basic horse heart cytochrome c (cyt c) at pH4.5 (net charge +17). The effect of salts consisting of imidazolium cations, 1-ethyl-3-methylimidazolium (EMIm+) and 1-butyl-3-methylimidazolium (BMIm+), and five anions chloride, bromide, iodide, nitrate, and thiocyanate on thermal and pH stability of cyt c was compared with the effect of corresponding sodium salts. Correlation between parameter of dTtrs/d [ion] (Ttrs; thermal midpoints) with surface tension changes of solvent in the presence of both imidazolium and sodium salts implies direct interaction between ions and proteins. Surprisingly, the imidazolium salts have more pronounced destabilization effect on highly positively charged cyt c than the corresponding sodium counterparts. Our analysis suggests the direct interaction of imidazolium cations with polypeptide chain, in analogy to guanidium cation, but the destabilization effect is significantly strengthened by decreased surface tension of imidazolium salt solvents.