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An effective, sensitive, relatively-fast and cost-effective method was developed to determine two types of selected organophosphorus pesticides (OPPs) including diazinon and chlorpyrifos in tomato, cucumber and agricultural water samples through applying MIL-101@ graphene oxide-reinforced hollow fiber solid-phase microextraction (MIL-101@GO-HF-SPME). The extracted analytes were desorbed via organic solvent and analyzed through high-performance liquid chromatography-ultraviolet detection (HPLC-UV) after extraction. MIL-101@ GO sorbent which was previously prepared and characterized was first dispersed in methanol by ultrasonication and then immobilized into the pores of a hollow fiber (HF). Several factors involved in MIL-101@GO-HF-SPME experiment such as desorption volume, ionic strength, desorption time, sample pH, extraction time and stirring rate were screened via Plackett-Burman design and then optimized through Box-Behnken design with the purpose of reaching the highest extraction efficiency. The above method showed a good linear range (1-500 μg L-1) with coefficient of determination higher than 0.9948, low limits of determination (LODs, 0.21 and 0.27 μg L-1), acceptable limits of quantifications (LOQs, 0.72 and 0.91 μg L-1), good enrichment factors (EFs, 49 and 41), acceptable absolute recoveries (AR%, 49 and 41%) and good spiking recoveries (88-104%) under the optimized condition at three different spiked levels for chlorpyrifos and diazinon, respectively. It is worth mentioning that due to the clean-up function of HF, there is no time-consuming sample pretreatment procedure (e.g. filtration or centrifugation) prior to the microextraction. Therefore, the presented method took advantage of both excellent adsorption performance of MIL-101@GO and the clean-up function of HF. The results confirmed that the presented method would be promising for the analysis of various types of these pesticides in environmental and vegetable samples.Here, a novel electrochemiluminescence biosensor based on potential-resolved strategy was firstly prepared for the detection of dual targets α2,3-sialylated glycans and α2,6-sialylated glycans. This is the first time that Au@BSA microsphere was used to connect with luminol to enhance its ECL intensity, and it can generate ECL signals at positive potential. Zeolitic Imidazolate Framework-8 (ZIF-8) and Meso-tetra (4-carboxyphenyl) porphyrin (TCPP) were linked using a one-pot method to synthesize a novel luminescent ZIF (L-ZIF) named TZZ, which can emit ECL signals at negative potential. Moreover, magnetite microspheres were used to construct a sandwich-type biosensor to obtain higher sensitivity and reduce background signals. In addition, the biosensor manufactured directly in solution have a wider linear range than constructed on electrode because it has more available space than the electrode surface. Due to the above advantages, the prepared ECL biosensor exhibited high sensitivity, stability and broader linear range, even for practical analysis. Therefore, the prepared ECL biosensor will become a promising method for determination of α2,3-sialylated glycans and α2,6-sialylated glycans in clinical applications in the future. What is more, it provides a potential method for detection of other multi-targets.Idiopathic pulmonary fibrosis (IPF) is an interstitial lung disease with unclear pathogenesis, for which diagnosis has been a great challenge. Recent researches have revealed that miR-3675-3p is a promising biomarker for IPF diagnosis. Herein, the present work describes a novel electrochemical microRNA biosensor for rapid and sensitive detection of miR-3675-3p based on multiple signal amplification strategies. First of all, fullerene (C60) is doped with poly(amidoamine) (PAMAM)-functionalized metal-organic framework (MOF) to form a new nanohybrid of C60@PAMAM-MOF, which exhibits more remarkable redox activity compared with the other two synthesized C60-based nanohybrids when triggered by tetraoctylammonium bromide (TOAB). C60@PAMAM-MOF also possesses a large specific surface area and abundant amino groups to anchor Au nanoparticles (AuNPs) for the immobilization of signal probe (SP) to form tracer label and enhance the electrochemical response signal. ODM208 cost In addition, core@shell Au-Pt nanoparticles (Au@PtNPs) are absorbed on chitosan-acetylene black (CS-AB) to act as sensing platform, which can promote electron transfer and increase the loading of capture probe (CP). Under optimum conditions, the proposed biosensor displays a wide linear range for miR-3675-3p from 10 fM to 10 nM, with a limit of detection (LOD) as low as 2.99 fM. More significantly, this biosensor shows a lower LOD and wider linear range than that of qRT-PCR, and its trial application in human serum shows favorable results, which exhibits a promising prospect for IPF diagnosis.Herein, a novel suspension array of polystyrene (PS) beads for simultaneous recognition and quantification of multiple cancer-associated microRNAs (miRNAs) using flow cytometry has been reported. The suspension array contained three moieties, streptavidin-modified PS beads, biotin-labeled substrate strands (17S) of the 17-8 DNAzyme and two split DNAzyme parts (PA, PB). 17S was labeled with 6-carboxyfluorescein (FAM) and Dabcyl on both sides of the ribonucleic acid. Once the target miRNAs appear, they can bind with the corresponding PA and PB to form an active secondary structure of DNAzyme. The active DNAzyme can cleave 17S and remove Dabcyl from the bead's surface, thus recovering the FAM's fluorescence intensity. Furthermore, the released target miRNA can autonomously move to the neighboring inactive DNAzyme for further cleavage, thus amplifying the fluorescence signal. Therefore, the target miRNAs can be quantified by reading the fluorescence intensity output from flow cytometry. The PS beads-based suspension array for the target miRNA in buffer shows good selectivity and high sensitivity. Via binding with a different pair of PA and PB, this suspension sensor array has successfully typed and quantified cancer-associated miRNAs of miR-21, miR-155, miR-335, and miR-122 in buffer and serum conditions.