Rosenbergmcgee4528

Caproate production by mixed culture fermentation (MCF) is economically attractive. Xylose is known as the second most abundant sugar in nature, however, producing caproate from xylose is never reported. In this study, caproate production from xylose by mesophilic MCF was firstly investigated. The results showed that as pH decreasing to 5.0, the caproate concentration was 2.06 g/L in a batch reactor and was between 0.45 and 1.07 g/L in a continuously stirred reactor. Microbial analysis illustrated that Caproiciproducens and Clostridium_sensu_stricto_12, as two main identified caproate producers, occupied over 50% and around 10% of mixed culture, respectively. Thus, caproate production from xylose was proposed via the fatty acid biosynthesis pathway, not the well-known reverse β-oxidation pathway. Selleck GSK'872 These unexpected differences from literatures gains more understanding about caproate production from organic substrates via MCF. Powdered activated carbon (PAC), lignite activated coke (LAC) and Fe-C carriers were applied to enhance CBFBRs to degrade targeted phenolics. In start-up stage, PAC and LAC equipped CBFBRs with higher environment adaptability and phenolic degradation capacity for phenol (>96%), p-cresol (>91%) and 3, 5-dimethylphenol (>84%) in comparison to Fe-C carrier. In recovery stage, the superior performance was also identified for CBFBRs in basis of PAC and LAC than Fe-C-based reactor. However, the Fe-C carrier assisted CBFBR with more stable degradation performance under impact loading. By comparing microbiomes, significantly enriched Brachymonas (54.80%-68.81%) in CBFBRs exerted primary role for phenolic degradation, and positively contributed to microbial network. Meanwhile, Geobacter in Fe-C-based reactor induced excellent impact resistance by enhancing interspecific electron transfer among microbes. Furthermore, the investigation on functional genes related to phenolic degradation revealed that anaerobic pathway accounted for demethylation procedure, while aerobic pathways dominated the phenolic ring-cleavage process. To avoid wastage of water resources and operating cost increases caused by the traditional start-up process of large amounts of dilution influent chemical oxygen demand (COD), a novel start-up process (NSP) was developed and verified with water hyacinth juice (WHJ) on an up-flow anaerobic sludge blanket (UASB) and modified internal circulation (MIC) reactor. Results show that UASB and MIC reactors were started successfully and that the MIC reactor exhibited a superior performance. The NSP time of the MIC reactor (46 days) was less than that of the UASB reactor (52 days), although the start-up organic loading rate (OLR) of the MIC reactor was higher than that of the UASB reactor. Interestingly, high-throughput sequencing analysis indicated that the reactor configuration significantly impacted the microbial diversity, however, the UASB and MIC reactors had similar predominant methanogens Methanosaeta and Methanosarcina. Therefore, acetoclastic methanogenesis is the primary pathway of methane formation during WHJ treatment. In the present work Chlorella pyrenoidosa, Scenedesmus abundans and Anabaena ambigua have been evaluated for their biomass, phycoremediation efficiency and biomethane production potential by cultivating them in the primary treated sewage waste water (PTSWW) under controlled conditions. By the end of 25-day experiment, up to 52-88% reduction was observed in the nutrient concentration from the 31 ratio of PTSWW. Co-digestion of microalgal biomass (dry) with cow dung was performed to estimate biomethane potential. Biogas yield of 618-925 ml g-1 VS with 48-65% of methane content was obtained employing the microalgal species cultivated in PTSWW. Microalgae appeared notably competent at nutrient sequestration from PTSWW with significant microalgal biomass productivity for biogas production. Energy balance studies revealed the feasibility of coupling the remediation with energy generation. High photosynthetic rate and biomass generation ability along with nutrient confiscation supports employment of microalgae as a potential next generation biofuel source with waste management. This study aimed to provide a low cost feasible pretreatment and enzymatic hydrolysis (EH) method for the effective dissolution of xylan and the high glucan digestibility of reed with a low enzyme loading. The combination of polyethylene glycol (PEG) 3000-enhanced EH and hydrothermal-alkaline/oxygen pretreatment was studied. Process conditions were optimized through response surface methodology. Three models of glucan conversion rate, pretreated solids yield and lignin removal rate were established, and their determination coefficient (R2) values were 0.9218, 0.7939, and 0.8156, respectively. The models and experiments were reliable and significant. The optimal conditions favored 94.5% xylan dissolution rate and 95.6% glucan digestibility by using a cellulase loading of 3 filter paper units (FPU)/g-pretreated solids, which obviously enhanced 30.7% of the glucan conversion rate. This method was applicable due to effective xylan dissolution, lignin removal, and EH with PEG 3000 addition, which can help saved 85% cellulase loading. Produced water (PW) generated during unconventional oil and gas extraction is characterized by very high total dissolved solids (TDS) that mainly consist of alkali and alkaline earth metals. Dominant PW management strategy (i.e., injection in Class II disposal wells) is scrutinized by regulatory agencies, and the public and PW treatment that enables high water and salt recovery (i.e., evaporation/crystallization) is being considered as an alternative. Produced water generated in the Marcellus Shale play also contains very high levels of Naturally Occurring Radioactive Material (NORM) in the form of Ra-226 and Ra-228, which is one of the key impediments for the recovery of high-quality salts. This study was designed to evaluate the efficiency of Ra-226 removal using co- and post-precipitation with barium sulfate to enable advanced PW treatment processes. High Sr/Ba molar ratios in PW lead to relatively low Ba2+ and Ra2+ removal, and Ba2+ concentration adjustment is necessary to achieve required treatment standards (i.