Dixonlindholm8215

Curcumin treatment showed reduced amount of fibrosis and significant reduction in level of liver biomarkers, reversal of antioxidant enzymes (SOD and GSH), MDA level, catalase activity and regain of electrolyte homeostasis. These findings confirm the protective role of curcumin in liver cirrhosis.The extraction procedure and antioxidant activity were investigated for total proanthocyanidins extracts from Abutilon theophrasti Medic. leaves collected in August, September and October. The maximum extraction yield was achieved with 90% ethanol, 80°C of heating reflux temperature, 149.94 min of extraction time and 60(ml/g) of the ratio of solvent and material, which were optimized by Box-Behnken Design of response surface method. Spectrophotometric study displayed that total proanthocyanidins content was (0.44±0.02)% (0.52±0.01)% and (0.59±0.01)% for August, September and October samples, respectively. The proanthocyanidins extracts exhibited much stronger antioxidant activity to scavenge ABTS and DPPH free radicals, and reduce ferric power than the control synthetic antioxidant BHT. The present findings suggest that the proanthocyanidins extract from Abutilon theophrasti Medic. leaves was a very interesting candidate for the research and development of natural and healthy antioxidant for the pharmaceutical and food industries.The connection of nearly all current antipsychotic drugs to their in vivo cytogenetic activity has not been yet fully investigated. Fluvoxamine, Valproic acid (VA) and Haloperidol (HLP) are three universally common consumed psychotic drugs whereas used to treat several psychiatric disorders. This study aims to investigate the cytogenetic effects of these three psychotropic drugs by evaluating the frequency of Sister Chromatid Exchanges (SCEs) and the Proliferation Rate Index (PRI) in cultured lymphocytes. Fifteen patients with psychiatric disorders (i.e. depression, bipolar and schizophrenia) consisting of smokers and non-smokers were included. Estimation of SCEs was used as a sensitive biomarker of the potential cytotoxicity, while PRI was used as a valuable marker of cytostatic activity. A significant increase of SCEs in the cultured lymphocyte of the smoker controls (P= 0.013) was found in compared to the non-smoker controls. This study found that there is no difference in the average of SCEs values in lymphocytes isolated from the smoker and non-smoker patients treated with Fluvoxamine, Valproic acid and Haloperidol (P> 0.05). A significant difference of PRI (P= 0.036) in the lymphocytes of smoker controls compared to those of the non-smoker controls were detected. find more This study also found a significant difference with respect to PRI between the three patient groups (P= 0.017). These results illustrated that treatment (monotherapy) of psychiatric patients with Fluvoxamine, Valproic acid, and Haloperidol exerts a significant cytostatic but not cytotoxic effect on their lymphocytes whereas these effects are intensified by smoking.To investigate the potential roles of the traditional Chinese medicine Yupingfengsan and Siwutang compound formula (YS) in chronic obstructive pulmonary disease (COPD) rats, wistar rats were assigned to control, YS-treated and COPD model groups. The COPD rats model were established by passive smoking and intratracheal instillation of lipopolysaccharide (LPS). Histological changes were detected by hematoxylin/eosin (HE) staining. Protein levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, transforming growth factor (TGF)-β1 and phosphorylated-smad2 (p-smad2) were determined by western blot assay. The activities of super oxide dismutase (SOD), glutathion peroxidase (GSH-Px) and the content of malondialdehyde (MDA) in the serum were estimated by biochemical methods. Relative mRNA levels of TNF-α, IL-6 and TGF-β1 were measured by quantitative real-time polymerase chain reaction (RT-PCR) analysis. The results showed that YS enhanced the above oxidase activity and decreased the yield of MDA, and reduced the levels of TNF-α, IL-6, TGF-β1 and p-smad2 in YS-treated COPD rats compared with the COPD rats. Our results suggested that YS produced the beneficial effects in COPD rats by antiinflammatory and antioxidative actions. Moreover, our research indicated that YS produced antiinflammatory effects in COPD rats by inhibiting the expression of inflammatory cytokines, possibly through suppressing the TGF-β1/Smad2 signaling pathway.The purpose of present study is to load Metformin HCl into pH-sensitive hydrogels to have sustained release over a period of time. The hydrogel was synthesized from naturally occurring polysaccharide pectin and monomer acrylic acid (AA) using ethylene glycol dimethacrylate (EGDMA) as cross-linker under controlled conditions for polymerization at 45°C for one hr, 50°C for two hrs, 55°C for three hrs, 60°C for four hrs and finally 65˚C for 12 hrs. Hydrogels were characterized for dynamic/equilibrium swelling, sol-gel fraction analysis, diffusion coefficient and percentage porosity. Hydrogels were tested by FTIR, XRD and SEM for structure and surface morphology respectively. Experimental in-vitro drug release data was applied to kinetic models. Formation of strong bonding between pectin and AA was supported by FTIR. The intensity of XRD peaks was reduced in non-loaded and loaded hydrogels compared to active drug substance. The non-loaded hydrogel showed discrete porous structure whereas loaded hydrogels were fibrous and smooth. Hydrogels showed higher swelling in the solutions of pH 6.5 and 7.5 as compared to in the solutions of pH 1.2 and 5.5. The diffusion coefficient decreases with the increase of AA and pectin concentrations. It was observed upon increasing the EGDMA concentration porosity decreases. The release of drug from all compositions of hydrogels took place through non-Fickian diffusion mechanism.Oenothera biennis L. is a perennial herb distributed across America, Asia, and Europe. The pharmacological effect of Oenothera biennis L. stem is poorly understood. We demonstrated that lipopolysaccharide (LPS)-induced the high production of inflammatory mediators nitric oxide (NO) and prostaglandin E2 (PGE2) and the pro-inflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-1β in peritoneal macrophages (PMs) were significantly inhibited by the crude extract The inflammation related signaling extra cellular signal-regulated ERK, P38 of MAPK and NF-kappaB (NF-κB) activated by LPS dramatically inhibited. In conclusion, our results suggested that the stems of Oenothera biennis L. possess a high anti-inflammatory property, thus, can be used in the industrial production of medicinal products as the raw material in the future.