Larssonaustin4752
4%), while the lowest prevalence belonged to
(0.6%). MLN4924 cell line
eggs and
were detected in one sub-source of water tanks.
larva, a free-living nematode, was observed in a soil sample.
The prevalence of parasitic infections in BuMusa Island was relatively low probably due to its hot and dry climate.
The prevalence of parasitic infections in BuMusa Island was relatively low probably due to its hot and dry climate.
Fascioliasis is a neglected zoonotic disease, caused by
species in human and livestock. We aimed to detect the seroprevalence of human fascioliasis Gorgan City, Golestan Province, northern Iran using ELISA method in 2017.
Overall, 612 serum samples were analyzed. A relevant questionnaire for demographic data was obtained for all cases. An indirect ELISA test was used to detect IgG antibodies against
in the sera. The data analysis was performed employing SPSS program version 21.
Eleven cases (1.79%) were seropositive for fascioliasis. The seroprevalence of fascioliasis was 1.9% and 1.1% among males and females, respectively. There was no statistically significant association between the fascioliasis and analyzed variables such as sex, age, residence, job, education, etc.
This study was conducted only on the people referring to the Reference Laboratory of Gorgan. It cannot be distributed to the whole city. Thus, due to importance of the disease, finding the seroprevalence of fascioliasis in a comprehensive survey in Golestan Province should be accounted in further studies.
This study was conducted only on the people referring to the Reference Laboratory of Gorgan. It cannot be distributed to the whole city. Thus, due to importance of the disease, finding the seroprevalence of fascioliasis in a comprehensive survey in Golestan Province should be accounted in further studies.
Application of chemotherapy to treatment of parasitic disease of man and animals can be problematic due to different adverse effects. As a result, there is an increasing interest in nanoparticles as new therapeutic tools against these diseases. This study was designed to evaluate the effect of selenium and copper oxide nanoparticles on
cysts in vitro, as well as comparing it to that of metronidazole.
The cysts were taken from the stools of patients in Urmia, Iran, during 2017-2018. The cysts were taken from stool and were concentrated and isolated on 0.85 M-sucrose. Then, selenium and copper oxide nanoparticles were prepared at concentrations of 0.15, 0.3, and 0.6 mg/ml. The effect of nanoparticle's various concentrations at 10, 15, 30, 60, and 180 min were evaluated and compared to control groups. Obtained data was recorded and statistically analyzed.
Copper oxide nanoparticles at a concentration of 0.6 mg/ml and selenium nanoparticles at a concentration of 0.3 mg/ml had the same effect as of metronidazole in killing of
cysts. The cytotoxic effects of selenium and copper oxide nanoparticles, compared with metronidazole, on
cysts, showed an increase of fatality rate due to extend exposure time and nanoparticle's concentration (
<0.05).
Selenium and copper oxide nanoparticles are as efficient as metronidazole, for killing
cysts in vitro.
Selenium and copper oxide nanoparticles are as efficient as metronidazole, for killing Giardia cysts in vitro.
Among the human parasitic diseases, malaria is the main cause of morbidity and mortality. To prevent the high mortality and tracking malaria elimination efforts, a prompt and sensitive diagnosis is essential. This study aimed to compare High-Resolution Melting (HRM) and microscopic methods to diagnose
and
.
Eighty-one blood samples were collected from patients with clinical symptoms who were suspect to malaria in Chabahar district, southeastern Iran and also, from those who were referred to Malaria National Laboratory in the Tehran University of Medical Sciences, Tehran, Iran. Microscopic examination and HRM method were used to the diagnosis of
parasites simultaneously.
Microscopic results revealed 45 positive cases (12
and 33
) out of 81 collected samples while according to HRM analysis results 11 and 33 samples were identified as
and
, respectively. HRM analysis also revealed 1 mixed infection of
and
HRM analysis provides a promising mean for simultaneous detection and discrimination of the
spp. especially in mixed infection cases.
HRM analysis provides a promising mean for simultaneous detection and discrimination of the Plasmodium spp. especially in mixed infection cases.
Free-living amoeba (FLA) are widely distributed in different environmental sources. The most genera of the amoeba are
,
and
The most common consequences of the infections in immune-deficient and immuno-competent persons are amoebic encephalitis and keratitis. The aim of this study was to investigate the presence of
spp. and
spp., isolated from the main agricultural water canal in Qazvin.
Totally, 120 water specimens were collected and later the specimens were cultured and cloned to identify positive samples. PCR amplification and sequencing were carried out to identify the isolated species as well as the genotypes of amoeba.
According to morphological surveys, 41.7% (50/120) of water specimens were positive for FLA. Molecular analysis revealed that 68.6% and 31.4% of
specimens were identified as T3 and T4 genotypes, respectively. Also, two species of
named as
(57.1%) and
sp. (42.8%) were identified. The results of pathogenicity assays demonstrated that 38.5% of T3 and 61.5% of T4 genotypes of
were highly pathogenic parasites.
The water flowing in the agricultural canal of the area is contaminated with potential pathogenic FLA, therefore, it is recommended that more attention to be paid towards proper treatment of water sources to prevent possible risk of the disease.
The water flowing in the agricultural canal of the area is contaminated with potential pathogenic FLA, therefore, it is recommended that more attention to be paid towards proper treatment of water sources to prevent possible risk of the disease.
Visceral leishmaniasis is the most severe form of leishmaniasis caused by
(
)
complex. Drug-resistant strains have been developed as a consequence of the current chemotherapeutic interventions, which has increased the need for advanced preventive and therapeutic strategies. A2-CPA-CPB
-recombinant strain of
, which is non-pathogenic to humans, was shown protective in live vaccine as well as its DNA vaccine counterpart in both murine and canine models.
We evaluated the effectiveness of these DNA and live vaccination harboring A2-CPA-CPB
in protecting hamsters against
infection using prime-boost regimens, namely DNA/DNA and Live/Live (n=9 hamsters per group). Cationic solid lipid nanoparticles (cSLN) were utilized as an adjuvant for DNA priming and electroporation for boosting DNA. At different time points post-challenge, parasite burden and body weight as well as humoral immune responses were measured.
Both immunization strategies partially protect hamsters against
challenge. This protective immunity is associated with remarkable decrease in parasite load in liver and spleen of vaccinated hamsters eight weeks after challenge compared to control group.
