Steelemccarty8741

miR-10a-5p showed the highest expression levels in AP. Overexpression of miR-10a-5p in LPS-challenged stem cells from the apical papilla resulted in down-regulation of messenger RNA levels of TNFA and up-regulation of interleukin IL10. RNA sequencing of K-562 cells treated withmiR-10a-5pmimics and inhibitors identified miR-10a-5p target genes associated with multiple pathways, including macrophage-mediated inflammation and coagulation pathways.

Over 100 miRNAs were differentially expressed in AP and appeared to be involved with modulation of genes in inflammatory and immune pathways. MiR-10a-5p was the most significantly up-regulated miRNA in AP and may play a critical role in suppressing inflammation and promoting healing.

Over 100 miRNAs were differentially expressed in AP and appeared to be involved with modulation of genes in inflammatory and immune pathways. MiR-10a-5p was the most significantly up-regulated miRNA in AP and may play a critical role in suppressing inflammation and promoting healing.

The aim of this study was to evaluate the volumes of total obturation and voids in different obturation techniques using nano-computed tomographic imaging. The null hypothesiswas that the obturation technique and the materials used have no effect on the total volume of obturation or the total volume of voids.

Fifty maxillary left central incisor 3-dimensional-printed replicas (TrueTooth; Dental Engineering Laboratories, Santa Barbara, CA) were instrumented and randomly assigned to 5 different obturation groups (n=10) single cone with EndoSequence Gutta-Percha Points (Brasseler USA, Savannah, GA) and Ribbon Sealer (Dentsply Sirona, Tulsa, OK) (SC1), single cone with BC 150 Series Gutta-Percha Points (Brasseler USA) and EndoSequence BC Sealer (Brasseler USA) (SC2), continuous wave with EndoSequence Gutta-Percha Points and Ribbon Sealer (CW), GuttaCorecarrier obturation (Dentsply Sirona) and Ribbon Sealer (GC), and cold lateral condensation with EndoSequence Gutta-Percha Points and Ribbon Sealer (CL). After obturation, nano-computed tomographic images were obtained, and volumetric analysis was performed. Statistical analysis using 1-way analysis of variance (ANOVA). The level of significance was set at 5% (P < .05).

The 1-way ANOVA for total obturation indicated a statistically significant effect of group on obturation. Post hoc tests revealed a significant difference between the SC2, CW, and CL groups compared with the SC1 and GC groups. Selleck BLU-667 The 1-way ANOVA for calculated voids indicated a statistically significant effect of group on voids. Post hoc tests revealed significant differences between the SC1 group and the GC and CL groups.

This study concluded that obturation technique and the materials used significantly affect the total volume of obturation material and potential for voids.

This study concluded that obturation technique and the materials used significantly affect the total volume of obturation material and potential for voids.

In this study, finite element analysis was used to evaluate the stress distributions in simulated mandibular molar teeth with various iatrogenic root perforation types after reparation with Biodentine (Septodont, Saint-Maur-des-Fossés, France) or mineral trioxide aggregate (MTA).

An extracted human mandibular molar tooth was scanned using a micro-computed tomographic device, and a 3-dimensional solid model was created. Then, 3 different iatrogenic perforation types (furcation perforation [FP], strip perforation [SP], and post drill perforation [PDP]) and 2 different repair materials (MTA and Biodentine [BD]) were simulated on the model. In addition, a sound tooth (ST) model (control) and a model left unrepaired for each type of perforation were created; then, access cavities were restored using resin composite, except for the sound tooth model. Consequently, a total of 10 experimental models were designed. An oblique force of 300 N angled at 45° to the occlusal plane was simulated. Evaluations of von Misef MTA and BD may reduce the risk of potentially harmful stress in root perforation regions.

This study evaluated the success rate of and tooth substance removal required for computer-guided preparation of endodontic access cavities.

Thirty acrylic typodont teeth with root canals (10 each of tooth numbers 11, 14, and 17) were randomly allocated to a study or control group (15 teeth per group). In the study group, teeth were fixed in acrylic resin and subsequently digitized using a laboratory scanner. A cone-beam computed tomographic scan was then taken. Access cavity preparations were planned virtually, and a template was 3-dimensionally printed. In the control group, access cavities were prepared using the conventional access technique. Tooth substance removal was assessed by weighing teeth before and after preparation. Volume loss was analyzed statistically by use of the Wilcoxon-Mann-Whitney test at a significance level of P < .05.

Using guided endodontics, 93.3% of root canals were located successfully compared with 100% of root canals using the conventional technique. In the control group, the mean tooth substance removal was 16.1 ± 3.7 mm³ for incisors, 44.2 ± 8.9 mm³ for premolars, and 99.3 ± 3.1 mm³ for molars. In the study group, significantly less tooth substance was removed; substance loss was 10.3 ± 1.1 mm³ for incisors, 29.3 ± 4.2 mm³ for premolars, and 51.8 ± 5.3 mm³ for molars.

The use of guided endodontics in normally calcified teeth enables the preservation of a significant amount of tooth substance. However, this advantage must be carefully balanced against a greater radiation burden and risk of perforation, higher costs, and more difficult debridement and visualization of the pulp chamber and root canals.

The use of guided endodontics in normally calcified teeth enables the preservation of a significant amount of tooth substance. However, this advantage must be carefully balanced against a greater radiation burden and risk of perforation, higher costs, and more difficult debridement and visualization of the pulp chamber and root canals.

This study investigated the effects of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) on serum inflammatory mediators of rats with pulp exposure-induced apical periodontitis.

Forty male Wistar rats were divided into the following groups control, untreated rats (C); control rats treated with ω-3 PUFAs (C-O); rats with pulp exposure-induced apical periodontitis (AP); and rats with pulp exposure-induced apical periodontitis treated with ω-3 PUFAs (AP-O). ω-3 PUFAs were administered orally once a day for 15 days before pulp exposure and continued for 30 days after pulp exposure. The rats were sacrificed, and then blood and jaw samples were collected. Blood analysis was conducted to determine the total number of leukocytes including neutrophils, monocytes, and lymphocytes. Proinflammatory cytokines tumor necrosis factor alpha, interleukin (IL) 6, and IL-17 were quantified by enzyme-linked immunosorbent assay. Histologic analysis was performed to confirm the development of apical periodontitis. The data were statistically evaluated using analysis of variance and the Tukey posttest.