Colemanhermann4852

Nitroaromatics belong to the group of toxic components of aerosol particles and atmospheric hydrometeors that enter the atmosphere through biomass burning and fuel combustion. In the present work, we report on the cytotoxic effects of a 2-, 3- and 4-nitrophenol mixture on a model eukaryotic-like cell membrane and compared it with in vitro cellular models BEAS-2B (immortalized bronchial epithelial cells) and A549 (cancerous alveolar epithelial cells). A selected model biomembrane comprised of DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine), DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) and POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) was studied. The electrochemical-based method, combined with atomic force microscopy (AFM) and phase-contrast microscopy imaging, allowed to get insights into the mechanism of cellular function disruption caused by airborne nitrophenols. The efficacy of the method is supported by the data obtained from in vitro experiments performed on cell models. The nitrophenol mixture exhibited cytotoxic effects at concentrations above 100 μg mL-1, as demonstrated by phase-contrast microscopy in real lung cell lines. Electrochemical impedance spectroscopy (EIS) revealed the formation of membrane defects at a nitrophenol concentration of 200 μg mL-1. AFM imaging confirmed the model membrane disintegration and phospholipids rearrangement in the presence of nitrophenols. These observations indicate that particle-bound nitrophenols induce substantial changes in cell membranes and make them more permeable to aerosol, resulting in major cellular damage in the lungs when inhaled. The study provides initial evidence of cellular membrane damage induced by three important nitrated phenols present in the environment.In this work, eight plants of Juncus sp. and ten of Salicornia europaea were used for an uptake assay of pharmaceuticals (flumequine, cirpofloxacin, enrofloxacin, carbamazepine, diclofenac and ibuprofen) by irrigation at three concentration levels 10 ng mL-1 (low level); 700 ng mL-1 (medium level) and 10 μg mL-1 (high level). Two plants irrigated with pharmaceutical-free water were set up as controls. For each level, two plants were watered every day with 50 mL (Juncus sp.) and every two days with 20 mL (Salicornia europaea) of aqueous solutions containing all the analytes at the described concentrations. Plants irrigated at 10 μg mL-1 were significantly the most affected, whereas the rest of the plants remained, in general, largely displayed no apparent physiological effects throughout the 30 days (Juncus sp.) and 21 days (Salicornia europaea) assays. Leaves and stems were cut every seven days and roots were collected at the end of the assay. The samples were lyophilized, submitted to a microwave assisted extraction using 5 mL of acetonitrilewater mixture (11, v/v) and they were analyzed (in triplicate) in a liquid chromatography-quadrupole time of flight mass spectrometry instrument. GPCR antagonist Most of the analytes were quantified in many of the samples corresponding to the three exposure levels with the highest concentrations obtained at high exposure levels. Ibuprofen was not detected in any sample and enrofloxacin, ciprofloxacin and diclofenac were not detected in the samples from Salicornia europaea.Neurotoxic insecticides are ubiquitous in aquatic ecosystems, frequently as part of complex mixtures. Freshwater gastropods are generally underrepresented in neurotoxicity evaluations and cumulative toxicity testing. This study investigates the behavioural and biochemical effects of acute exposures to the carbamate carbaryl, the organophosphate chlorpyrifos, and the neonicotinoid acetamiprid on the freshwater gastropod Chilina gibbosa. First, we evaluated behavioural neurotoxicity and cholinesterase (ChE), carboxylesterase (CE), and glutathione S-transferase (GST) activities in acute (48h) single-chemical exposures to increasing concentrations of carbaryl (0.5-500 μg L-1), chlorpyrifos (10-7500 μg L-1), and acetamiprid (1-10000 μg L-1). We then studied the effects of acute (48h) exposures to binary mixtures of carbaryl and chlorpyrifos equivalent to 0.5, 1, and 1.5 ChE 48h-IC50. None of the insecticides caused severe behavioural neurotoxicity, except for a significant lack of adherence by 5000 μg L-1 chlorpyrifos. Carbaryl caused concentration-dependent inhibition of ChEs (NOEC 5 μg L-1; 48h-IC50 45 μg L-1) and CEs with p-nitrophenyl butyrate as substrate (NOEC 5 μg L-1; 48h-IC50 37 μg L-1). Chlorpyrifos caused concentration-dependent inhibition of ChEs (NOEC 50 μg L-1; 48h-IC50 946 μg L-1) but did not affect CEs (NOEC ≥7500 μg L-1). Carbaryl-chlorpyrifos mixtures inhibited ChEs additively, inhibited CEs with p-nitrophenyl butyrate, and did not affect behaviour. GST activity was not affected by single or mixture exposures. Acute exposure to acetamiprid did not affect any of the endpoints evaluated. This study provides new information on carbaryl, chlorpyrifos, and acetamiprid toxicity on C. gibbosa, relevant to improve gastropod representation in ecotoxicological risk assessment.Since iron oxide minerals are ubiquitous in natural environments, the release of graphene oxide (GO) into environmental ecosystems can potentially interact with iron oxide particles and thus alter their surface properties, resulting in the change of their transport behaviors in subsurface systems. Column experiments were performed in this study to investigate the co-transport of GO nanoparticles and hematite colloids (a model representative of iron oxides) in saturated sand. The results demonstrated that the presence of hematite inhibited GO transport in quartz sand columns due to the formation of less negatively charged GO-hematite heteroaggregates and additional deposition sites provided by the adsorbed hematite on sand surfaces. Contrarily, GO co-present in suspensions significantly enhanced the transport of hematite colloids through different mechanisms such as the increase of electrostatic repulsion, decreased physical straining, GO-facilitated transport of hematite (i.e., highly mobile GO nanoparticles served as a mobile carrier for hematite). We also found that the co-transport behaviors of GO and hematite depended on solution chemistry (e.g., pH, ionic strength, and divalent cation (i.e., Ca2+)), which affected the electrostatic interaction as well as heteroaggregation behaviors between GO nanoparticles and hematite colloids. The findings provide an insight into the potential fate of carbon nanomaterials affected by mineral colloids existing in natural waters and soils.