Colonmooney2137

The findings showed that nutrients were an important factor that promoted PBDE absorption in the gastrointestinal tract. Therefore, it is important to focus on a novel dietary strategy of food consumption with contaminant compounds to protect human health.The waters and wastewaters around industrial areas are heavily polluted and have adverse effects on the ecosystems. The present study is mainly focused on the electropolymerization of ortho-phenetidine and co-deposited on a steel wire along with graphene oxide nanosheets as a novel coating for solid-phase microextraction of polycyclic aromatic hydrocarbons (PAHs) from aqueous media prior to gas chromatography-mass spectrometry. PAHs are composed of multiple aromatic rings which have been linked to skin, lung, bladder and liver. Cancer is a primary human health risk of exposure to PAHs. To obtain a firm and stable coating, several empirical factors relevant to the electrochemical process were investigated. click here Characterization for chemical structure and surface morphology of the synthesized nanocomposite was conducted with FT-IR spectroscopy and FE-SEM, respectively. XRD and TGA were applied to study the other properties of the nanocomposite. Some essential items involved in microextraction process were also checked in details. Under optimized case, validation parameters were assessed. Wide linearity (0.005-5.0 ng mL-1), low detection limits (0.4-4.3 pg mL-1) and good repeatability (3.6-9.5%) and reproducibility (7.6-11.8%) were achieved. The developed method was utilized to analyze contaminated real samples such as wastewater samples from coal processing industries and agricultural water samples collected from the vicinity of the industry in different seasons and high recoveries were obtained, finally.In this study, the production and compositional analysis of exopolysaccharides produced by Bacillus cereus KMS3-1 grown in metal amended conditions were investigated. In addition, the metal adsorption efficacy of exopolysaccharides (EPS) produced by KMS3-1 strain was evaluated in a batch mode. Increased production of exopolysaccharides by KMS3-1 strain was observed while growing under metal amended conditions (100 mg/L) and also, the yield was in the order of Pb(II)>Cu(II)>Cd(II)>Control. Characterization of EPS using FT-IR, XRD, and SEM analysis revealed that the EPS can interact with metal ions through their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O) and assist the detoxification process. Further, equilibrium results were fitted with the Langmuir model and notably, the maximum adsorption capacity (Qmax) of EPS for Cd(II), Cu(II), and Pb(II) found to be 54.05, 71.42, and 78.74 mg/g, respectively. To the best of our knowledge, EPS demonstrating proficient metal adsorption was substantiated by XRD analysis in this study. Owing to good adsorbing nature, the exopolysaccharides could be used as chelating substances for wastewater treatment.Androgens and estrogens often co-exist in aquatic environments and pose potential risks to fish populations. However, little is known about the endocrine disrupting effects of the mixture of androgens and estrogens in fish. In this study, transcriptional level of target genes related to the hypothalamic-pituitary-gonadal-liver (HPGL) axis, sex hormone level, VTG protein concentration, histology and secondary sex characteristic were assessed in the ovaries and livers of adult female western mosquitofish (Gambusia affinis) exposed to 17β-estradiol (E2), testosterone (T), and mixtures of E2 and T for 91 days. The results showed that the transcriptional expression of cytochrome P450, family 19, subfamily A, polypeptide 1a (Cyp19a1a) was suppressed in the 200 ng/L T treatment and the 50 ng/L E2 + 200 ng/L T treatment in the ovaries. Steroidogenic acute regulatory protein (Star) and Cyp11a1 showed a similar expression pattern in the T treatment to its corresponding T + E2 mixtures. In the ovaries, the concentrations of 17β-estradiol and testosterone were decreased in most treatments compared with the solvent control. VTG protein was induced in all steroid treatment. However, exposure to T or E2 + T mixture did not cause the abnormal cells of the ovaries and livers and an extension of the anal fins in female G. affinis. This study demonstrates that chronic exposure to E2, T and their mixtures affects the transcripts of genes in the HPGL axis, steroid hormone level and VTG protein concentration in the ovaries and livers, but fails to cause the histopathological effect of the ovaries and livers and alter the morphology of the anal fins in G. affinis.Ammonia (NH3) is a major pollutant in livestock houses and atmospheric environment. It has been demonstrated that NH3 can cause a series of damage to animals and human. However, toxicity evaluation of NH3 on farm animals was rarely reported, especially in the intestinal microflora. Therefore, in this study, twenty-four 125-day-old fattening pigs were randomly divided into 4 groups control group, NH3 group (88.2 mg m-3 less then NH3 concentration less then 90.4 mg m-3), Se group (Se content 0.5 mg kg-1), and NH3 + Se group (88.2 mg m-3 less then NH3 concentration less then 90.4 mg m-3, Se content 0.5 mg kg-1), and the effects of NH3 and L-Selenomethionine on the microbiota composition in the jejunum and the levels of inflammatory markers in feces of fattening pigs were examined by 16S rDNA and ELISA, respectively. Our results showed that the content of Matrix metalloproteinase-9 (MMP-9), Myeloperoxidase (MPO), Lactoferrin (LTF) and Calprotectin in the ammonia group (A group) were significantly elevated compared to the control group, and the content of MMP-9, MPO, LTF and Calprotectin in the A + Se group were significantly reduced. A significant difference in microbiota composition in the phylum, class, family and genus levels was found in the A group and the NH3 + Se group. There was a negative correlation between Streptococcus and Calprotectin. Our results indicated that excessive NH3 inhalation could cause changes in inflammatory markers and beta diversity of intestinal microflora in fattening pigs. We found there was a positive correlation between MPO and Pseudomonas. In addition, we first proposed that L-Selenomethionine could improve the imbalance of microbial flora and the inflammatory injury caused by NH3. Changes in intestinal microflora and inflammatory markers can be used as important indicators to evaluate NH3 toxicity, and studying changes in intestinal microflora is also an important mechanism to reveal NH3 toxicity.