Conradgardner8948

Muscle force-length dynamics are governed by intrinsic contractile properties, motor stimulation and mechanical load. Although intrinsic properties are well-characterised, physiologists lack in vitro instrumentation accounting for combined effects of limb inertia, musculoskeletal architecture and contractile dynamics. We introduce in vitro virtual-reality (in vitro-VR) which enables in vitro muscle tissue to drive a musculoskeletal jumping simulation. In hardware, muscle force from a frog plantaris was transmitted to a software model where joint torques, inertia and ground reaction forces were computed to advance the simulation at 1 kHz. To close the loop, simulated muscle strain was returned to update in vitro length. We manipulated 1) stimulation timing and, 2) the virtual muscle's anatomical origin. This influenced interactions among muscular, inertial, gravitational and contact forces dictating limb kinematics and jump performance. We propose that in vitro-VR can be used to illustrate how neuromuscular control and musculoskeletal anatomy influence muscle dynamics and biomechanical performance. © 2020. Published by The Company of Biologists Ltd.Disassembling the parasitic spores and acquiring the main subunits is a prerequisite for deep understanding of the basic biology of parasites. Herein we present a fast and efficient method to dissect the myxospores in a few steps, which mainly involved sonication, sucrose density gradient and Percoll density gradient. We tested our method on three myxozoans species and demonstrated this method allows the dismembering of myxospores, isolation of intact and clean nematocysts and shell valves within 2h by low-cost. This new tool will facilitate subsequent analyses and enable a better understanding of the ecological and evolutionary significance of parasitic spores. © 2020. Published by The Company of Biologists Ltd.Many species are able to vocally recognise individual conspecifics and such a capacity seems widespread in oscine songbirds. The exact acoustic feature used for such recognition is often not clear. In the zebra finch (Taeniopygia guttata), the song motif is composed of few syllables repeated in a fixed sequential order and song bouts include several repetitions of the motif. Here we used an operant discrimination task, the GO/NOGO procedure, to show that zebra finches are capable of vocal individual recognition even if the bird has to distinguish males that all produce an imitation of the same song model. Furthermore, we studied whether such individual vocal recognition was based on spectro-temporal details of song syllables, i.e. the local fine structure of the song, or on the sequential order in which song syllables are arranged in the song bout. To this end, we trained male and female zebra finches to discriminate songs of one male conspecific from those of four others. After learning this baseline discrimination, subjects were exposed to a novel set of stimuli originating from the same individuals, in order to test for their capability to generalise. Subjects correctly classified those novel stimuli illustrating their ability for individual vocal recognition. Then they were exposed to hybrid stimuli combining the syllable sequences of one individual with the spectro-temporal features of another. Behavioural responses of subjects to hybrid stimuli suggest that they rely on spectro-temporal details of syllables and might pay less attention to syllable sequences for individual vocal recognition. selleck chemicals llc © 2020. Published by The Company of Biologists Ltd.BACKGROUND The benefit of endovascular treatment (EVT) is highly time-dependent, and treatment delays reduce patients' chances to achieve a good outcome. In this survey-based study, we aimed to evaluate current in-hospital EVT workflow characteristics across different countries and hospital settings, and to quantify the time-savings that could be achieved by optimizing particular workflow steps. METHODS In a multinational survey, neurointerventionalists were asked to provide specific information about EVT workflows in their current working environment. Workflow characteristics were summarized using descriptive statistics and stratified by country and physician characteristics, such as age, career stage, personal and institutional caseload. RESULTS Among 248 respondents from 48 countries, pre-notification of the neurointerventional team was used in 70% of cases. The emergency department (ED) and CT scanner, and the CT scanner and neuroangiography suite, were on different floors in 23% and 38%, respectively. Redundant procedures in the ED were often routinely performed, such as chest x-rays (in 6%). General anesthesia was the most frequently used anesthesia protocol for EVT (42%), and an anesthesiologist was available in 82% for this purpose. 52% of the participants used a pre-prepared EVT kit. CONCLUSION The current structure of EVT workflows offers possibilities for improvement. While some bottlenecks, such as the spatial department set-up, cannot easily be resolved, pre-notification tools and pre-prepared EVT kits are more straightforward to implement and could help to reduce treatment delays, and thereby improve patient outcomes. © Author(s) (or their employer(s)) 2020. No commercial re-use. See rights and permissions. Published by BMJ.INTRODUCTION There has been a phenomenal worldwide increase in the development and use of mobile health applications (mHealth apps) that monitor menstruation and fertility. Critics argue that many of the apps are inaccurate and lack evidence from either clinical trials or user experience. The aim of this scoping review is to provide an overview of the research literature on mHealth apps that track menstruation and fertility. METHODS This project followed the PRISMA Extension for Scoping Reviews. The ACM, CINAHL, Google Scholar, PubMed and Scopus databases were searched for material published between 1 January 2010 and 30 April 2019. Data summary and synthesis were used to chart and analyse the data. RESULTS In total 654 records were reviewed. Subsequently, 135 duplicate records and 501 records that did not meet the inclusion criteria were removed. Eighteen records from 13 countries form the basis of this review. The papers reviewed cover a variety of disciplinary and methodological frameworks. Three main themes were identified fertility and reproductive health tracking, pregnancy planning, and pregnancy prevention.