Robinsonwrenn6712

The method proposed in this work showed good performances, including wide linearity (14.6-250 ng/g) with high correlation coefficients (R ≥ 0.9991), low limits of detection (LODs, 0.7-6.5 ng/g for all SAs), satisfactory precision (Intra-day RSDs ≤ 3.4%, inter-day RSDs ≤ 4.7%), and high accuracy (recovery 83.4-103.8%). Furthermore, benefiting from the mesoporous structure and the Zr cluster center as well as the stability, UiO-67 shows great potential to be an excellent adsorbent of SPE for extraction of SAs from other complex matrices. Two thermo-responsive molecularly imprinted polymers (MHNTs@MIP and MCNTs@MIP) for the selective extraction of sterigmatocystin have been prepared on the surface of the magnetic halloysite nanotubes (MHNTs) and magnetic carbon nanotubes (MCNTs), respectively. 1, 8-dihydroxyanthraquinone, n-isopropyl acrylamide, methacrylic acid, ethylene dimethacrylate and dimethyl sulfoxide were used as the dummy template, thermo-sensitive functional monomer, co-monomer, cross-linker and porogen, respectively. The magnetic properties, adsorption properties as well as the temperature responsive behaviors of MHNTs@MIP and MCNTs@MIP were systematically studied and compared for the first time. Enough saturation magnetizations of MHNTs@MIP (9.42 emu/g) and MCNTs@MIP (10.54 emu/g) were obtained. MHNTs@MIP and MCNTs@MIP also showed controllable adsorption and release behaviors to sterigmatocystin in response to the temperature change (35 °C and 20 °C). selleck inhibitor Compared with MCNTs@MIP, MHNTs@MIP had higher adsorption affinity (KL = 0.120 L/mg), higher adsorption kinetic (K2 = 0.0100 g/(mg•min)) and higher imprinting factor (5.22) to sterigmatocystin. These results indicated that MHNTs@MIP was favorable adsorbent for the selective separation of sterigmatocystin. Furthermore, the elution conditions of MHNTs@MIP were optimized by response surface methodology. Under the optimal conditions, MHNTs@MIP coupled with high performance liquid chromatography were successfully applied to the selective recognition, purification, enrichment and detection of sterigmatocystin in wheat samples. The recoveries were calculated from 88.62% to 102.9% with RSDs less than 3.5 % and limit of detection of 1.1 μg/kg. This work provided a suitable carrier for the preparation of imprinted polymers and a practical approach for highly selective recognition and determination of analytes in real samples. V.A new platform was developed for determination of drugs in plasma without extraction or instrumental analysis just using TLC, smart phone digital camera and free image processing software. Lamotrigine, antiepileptic drug was used as model analyte. The proposed platform depends on using oil-in-water (O/W) microemulsion to isolate the drug from plasma proteins and using water-in-oil (W/O) microemulsion as mobile phase for TLC which results in complete separation between lamotrigine and plasma as viewed under UV lamp. The composition of both microemulsions was optimized using Taguchi orthogonal array and Plackett- Burman design. The optimal (O/W) microemulsion predicted composition was 0.01 mL Butyl acetate, 4 mL butanol, 0.925 gm SDS and 8.6 mL water while the (W/O) mobile phase microemulsion was 9 mL Butyl acetate, 1 mL butanol, 0.25 gm SDS, 0.25 mL water. Separation was carried out on a silica gel 60F-254 plate eluted with the (W/O) microemulsion in about 30 min development time. The images of TLC plates were processed using 4 different programs, by comparing their results it was found that "integrated density" measured by Fiji software was the most accurate response that could measure the concentration of lamotrigine in spiked plasma in the range of (20-200) ng/spot. This method was applied also for determination of lamotrigine in lamictal® tablet dosage form using the same mobile phase. The precision of the method was satisfactory; the maximum value of relative standard deviations did not exceed 1.5%. While the accuracy was proved by the low values of % error and high values of recovery. V.Lateral wedge insoles (LWI) have been proposed to reduce the knee adduction moment (KAM) during walking; a biomechanical modification notably sought in case of medial knee osteoarthritis. However, the inter-individual inconsistency in KAM changes with LWI limits their therapeutic use. Although the foot progression angle (FPA) has been frequently discussed in KAM modifications literature, there is a lack of data regarding a possible relationship between this gait measure and changes in KAM with LWI. This study aimed to test if KAM changes with LWI differ with respect to the natural FPA and to compare KAM-related variables between individuals walking with smaller and larger natural FPA. Twenty-two healthy participants (14 males, 24 ± 3 years, 22.7 ± 2.7 kg/m2) underwent gait analysis with and without LWI. They were divided into two groups based on their natural FPA, and changes in KAM 1st peak, KAM impulse, and KAM-related variables were compared between groups. KAM 1st peak and impulse decreased with LWI in the smaller natural FPA group (p ≤ 0.006), while only KAM impulse decreased in the larger natural FPA group (p  less then  0.001). The difference in KAM 1st peak changes was explained by a less reduced lever arm in participants walking with larger natural FPA. In conclusion, this study brought new insight into the variability in KAM response to LWI. If the findings are confirmed in patients with medial knee osteoarthritis, the FPA could become a simple measure to help identify the patients more likely to reduce their KAM with LWI. The tibiofemoral compressive forces experienced during functional activities are believed to be important for maintaining tibiofemoral stability. Previous studies have shown that both knee-spanning and non-knee-spanning muscles contribute to tibiofemoral joint compressive forces during walking. However, healthy individuals typically engage in more vigorous activities (e.g. jumping and cutting) that provide greater challenges to tibiofemoral stability. Despite this, no previous studies have investigated how both knee-spanning and non-knee-spanning muscles contribute to tibiofemoral compressive loading during such tasks. The present study investigated how muscles contributed to the medial and lateral compartment tibiofemoral compressive forces during sidestep cutting. Three-dimensional marker trajectories, ground reaction forces and muscle electromyographic signals were collected from eight healthy males whilst they completed unanticipated sidestep cutting. OpenSim was used to perform musculoskeletal simulations to compute the contribution of each lower-limb muscle to compressive loading of each compartment of the knee.