Hunterkuhn7605

these samples with a low quantity of DNA were highlighted as well. We concluded that the Casework Direct Kit was much more efficient for processing touch DNA samples than DNA IQ.The aim of this study was to evaluate the effect of aqueous solutions of deep eutectic solvent, Cholinium ChlorideUrea ([Ch]ClU) at 50 wt% and 20 wt%, using different molar ratios (11, 21 and 12) on the enzymatic hydrolysis of xylan for xylose production and its subsequent bioconversion into xylitol using a recombinant yeast strain. The lowest xylan conversion into xylose (45%) was obtained using 12 [Ch]ClU molar ratio. On the other hand, the 11 [Ch]ClU molar ratio, at 20 wt% in water, improved this conversion, achieving the highest xylose yield (81.4%). The xylitol production was then optimized with [Ch]ClU (11) at 20 wt% by simultaneous saccharification and fermentation process, attaining 23.67 g/L, corresponding to 66.04% of xylitol yield. This study reveals the possibility of using xylan solubilized in DES aqueous solutions directly for xylitol production, thus assembling a one-step process.This study analyzed the effect of TiO2 on the growth and astaxanthin yield of P. rhodozyma PR106. Subsequently, proteomics method was used to analyze the proteins changes of the strain under TiO2 treatment, to investigate the metabolic mechanism of the active oxygen generator TiO2 promoting the astaxanthin synthesis in P. rhodozyma. The results showed that TiO2 caused oxidative stress response in P. rhodozyma, and astaxanthin yield was 14.74 mg/L, which was 2 times of the control group; while, TiO2 had no effect on biomass and apoptosis of the cells. Proteomics analysis and parallel reaction monitoring (PRM) technology initially explored that bud-site selection protein (BUD22), ubiquitin-40s ribosomal protein s31 fusion protein, cell cycle control protein, C-4 methyl sterol oxidase and glutaredoxin were associated with astaxanthin synthesis.In this study, chemoenzymatic conversion of Sorghum durra stalk (SDS) into furoic acid was developed by a sequential microwave-assisted solid acid conversion and immobilized whole-cells biocatalysis method. Dry dewaxed SDS (75 g/L) was catalyzed into furfural at 57.8% yield with heterogeneous Sn-argil (2.0 wt% dosage) in n-ethyl butyrate-H2O (11, vv) biphasic system using a microwave (600 W) for 10 min at 180 °C. In this biphasic media (pH 6.5), SDS-derived furfural (125.0 mM) was biologically oxidized to furoic acid by immobilized Brevibacterium lutescens cells harboring furfural-oxidizing activity at 30 °C, and furfural was wholly transformed to furoic acid within 24 h. Finally, the recovery and reuse of the Sn-argil catalyst and immobilized biocatalysts were conducted for synthesizing furoic acid from SDS in the biphasic system. This chemoenzymatic route can be attractive for furoic acid production.The current study investigated an intergenerational transmission model between maternal overprotection and child anxiety across three generations, among a sample of substance-using women and their children. Participants included 183 mother-child pairs. Mothers reported overprotective behaviors of their own mothers, as well as their own anxiety symptoms and substance use at baseline. Mothers reported their adolescents' internalizing behaviors, and adolescents reported their mothers' overprotection, five times over 1.5 years. Growth curve models showed that overprotection among the first generation (G1) mothers was related to anxiety among the second generation (G2) mothers and sequentially the rate of change of overprotection among G2 mothers. Baseline overprotection among G2 mothers was related to baseline internalizing problems among the third generation (G3). More importantly, substance use among G2 mothers moderated the link between the rate of change in overprotection among G2 mothers and the rate of change in internalizing problems among G3 children. Findings uncover the intergenerational transmission model of overprotection-anxiety and shed light upon the complex relations among anxiety, substance use, and parenting in substance-using families.Attentional bias (AB) refers to increased allocation of attention on threat stimuli when compared to neutral stimuli. It is not clear if AB occurs in subjects with obsessive-compulsive disorder (OCD). We tested AB for symmetry and cleaning symptoms of OCD. Sixty-two patients with OCD and 40 healthy controls matched by gender, age and IQ, completed a computerized dot-probe task where two pictures (with symmetry or cleaning related content) were shown. The probe appeared in the location previously occupied by one of the pictures. Within-subjects linear mixed-effect models were used to investigate the effects of the factors group (patients vs controls), OCD dimension (cleaning vs symmetry), task condition (neutral, congruent and incongruent), and the interaction among them. We also correlated AB scores with the clinical and demographic variables. No positive interaction resulted among the factors, but positive results were observed in group and condition, separately. Patients were significantly slower than controls (p-value = 0.014) (an effect that was accounted for by depression and anxiety symptoms and comorbidity) and the neutral condition was significantly faster when compared the other two conditions (congruent and incongruent, p-value = 0.013). No association was found between AB scores and clinical symptoms. There was no AB toward specific, content-related, stimuli in this sample of OCD patients.Background Alterations in the levels of gamma-aminobutyric acid (GABA) and glutamate + glutamine (Glx), which are major inhibitory and excitatory neurotransmitters, respectively, are frequently associated with insomnia. Previous reports also suggested the involvement of the anterior cingulate cortex (ACC) and medial prefrontal cortex (mPFC) in insomnia and shorter sleep duration. RO4929097 In the current study, we investigated whether the GABA and Glx levels were altered in the ACC/mPFC in subclinical insomnia while focusing on the sleep duration. Methods We examined levels of GABA and Glx in the ACC/mPFC of the brain with magnetic resonance spectroscopy in 166 individuals with subjective sleep complaints but without a diagnosis of insomnia. Participants were divided into two groups according to sleep duration (≥6 h/night n = 79 vs. less then 6 h/night n = 74), which was measured using a wrist-worn actigraphy. Working memory function and overall subjective sleep quality were assessed with a computerized neuropsychological test and self-report questionnaire, respectively.