Stantonwaugh2008

The 2016 Presidential Commission to Eliminate Child Abuse and Neglect Fatalities identified systematic review of all cases of near-fatal child maltreatment as a necessary step towards prevention of child maltreatment fatalities. A critical barrier to adoption of this recommendation is the lack of a standard definition of "near-fatality" in the context of suspected child maltreatment.

To develop a consensus definition of near-fatal child maltreatment to be used in practice, policy, and research.

A multidisciplinary expert panel of 23 individuals from across the U.S. including child abuse pediatricians, pediatric intensivists, pediatric emergency medicine physicians, child welfare administrators, child welfare researchers, and child injury/fatality researchers.

A modified Delphi process reflecting an iterative process of 3 rounds of surveys of expert opinion, statistical summary of survey response, and feedback of summary statistics. Consensus was defined as 75 % of panelists ranking an element as requiespond to trends in near-fatal child maltreatment at local, regional, and national levels.The developing public interest in traditional medicine, especially plants-based drug, has prompted extensive research on the potential of naturally existing compounds. Among these compounds, curcumin is currently one of the most studied substances. In this study, we elaborate the physical properties of diarachidonyl phosphatidyl choline (DAPC) liposome using fluorescence method, where curcumin at low concentration was used as a probe molecule. In the first place, the phase transition temperature of DAPC was determined by following the fluorescence intensity of curcumin as a function of temperature, along with evaluating the effect of concentration of curcumin in the presence or absence of chitosan oligosaccharide lactate as an additional protective layer. selleck chemicals llc On the other hand, quenching reactions using CPB and KI as quenchers reflected the ease of entry of different concentrations of these quenchers to the curcumin located in the hydrophobic core of the liposome which give new insight about the lipophilicity and permeability of the DAPC membrane. Finally, the partition coefficient analysis was investigated. It was concluded that curcumin has a higher partition coefficient at a temperature above the phase transition temperature of DAPC liposomes where the liposome is in the fluid liquid crystalline phase. Modulation of liposomes properties in the presence of chitosan oligosaccharide lactate layer was for the first time investigated. Chitosan oligosaccharide lactate acts as protecting layer without changing the phase transition temperature, but it affects the membrane permeability depending on solid gel and liquid crystalline phase.A novel form of magnetic bead, namely antibody-coated magnetic lipid nano-vehicle (AMLV), was synthesized by embedding Fe3O4 nanoparticles into an amphiphilic antibody-modified liposome as a high-performance circulating tumor cell (CTC) hunter. The CTC capture performance of AMLV was validated based on an enlarged patient sample (including 318 colorectal, 78 breast, 77 lung and 55 liver cancer patients) with high detection rate. The preliminary comparison with Cellsearch was also conducted, indicating that the cell membrane-semblance AMLVEpCAM showed higher capture performance for different kinds of EpCAM-expressed circulating tumor cells in the peripheral blood (4.4 ± 1.2-fold for AMLVEpCAM vs CellsearchTM, n=5, P less then 0.001). Moreover, the AMLVEpCAM-isolated CTCs could be used as a functional material to provide various clinical information for tumor patients and work as an alternative of tumor tissue to conduct gene analysis after conventional PCR amplification.Curcumin and two bivalent compounds, namely 17MD and 21MO, both obtained by conjugation of curcumin with a steroid molecule that acts as a membrane anchor, were comparatively studied. When incorporated into 1,2-dipalmitoyl-sn-glycero-3-phosphocholine the compounds showed a very limited solubility in the model membranes. Curcumin and the two bivalent compounds were also incorporated in membranes of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine and quenching the fluorescence of pure curcumin or of the curcumin moiety in the bivalent compounds by acrylamide it was seen that curcumin was accessible to this water soluble quencher but the molecule was somehow located in a hydrophobic environment. This was confirmed by quenching with doxyl-phosphatidylcholines, indicating that the curcumin moieties of 17MD and 21MO were in a more polar environment than pure curcumin itself. 1H NOESY MAS-NMR analysis supports this notion by showing that the orientation of curcumin was parallel to the plane of the membrane surface close to C2 and C3 of the fatty acyl chains, while the curcumin moiety of 17MD and 21MO positioned close to the polar part of the membrane with the steroid moiety in the centre of the membrane. Molecular dynamics studies were in close agreement with the experimental results with respect to the likely proximity of the protons studied by NMR and show that 17MD and 21MO have a clear tendency to aggregate in a fluid membrane. The anchorage of the bivalent compounds to the membrane leaving the curcumin moiety near the polar part may be very important to facilitate the bioactivity of the curcumin moiety when used as anti-Alzheimer drugs.Using yeast as organic template and PDDA/PSS with opposite charge as polyelectrolyte, CaCO3 was deposited on yeast cells by Layer-by-Layer self-assembly method, and then porous calcium carbonate hybrid microspheres (CaCO3-HMPs) were prepared by calcination. The CaCO3-HMPs were characterized by FT-IR, XRD, SEM and TG. It was found that the prepared CaCO3-HMPs were nearly spherical, with visible pores on the surface, small particle size uniformity (d = 3 μ m) and good dispersion. Doxorubicin hydrochloride (DOX) was used as the model drug to study drug loading and release properties of CaCO3-HMPs. Then, the drug loading, DOX release under different pH conditions, and the degradation of CaCO3-HMPs under different pH conditions were investigated. The drug release test results showed that the DOX-loaded microspheres released more drugs (99 %) at pH = 4.8 than pH = 7. It indicated that the CaCO3-HMPs were pH sensitive. The cytotoxicity of DOX-loaded microspheres was also studied. It was found that CaCO3-HMPs had good biocompatibility.