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In this study, findings indicated that effects of increased digestible AA density on FI, performance, and processing yield are specific to strain and grow-out temperature, but the optimum response was attained for both lines with diets containing 110 to 120% AA levels (3.48-3.80 g dLys/Mcal) during the 22 to 42 d finisher period.The objective of this study was to evaluate the effects of different levels of dietary natural astaxanthin (ASTA) (from the microalga Haematococcus pluvialis) and storage at 4°C and 25°C on the quality of eggs from laying hens. Nongda No. 3 laying hens (n = 450) were randomly allocated to 1 of 5 dietary treatments. Each treatment had 6 replicates of 15 hens each. All birds were assigned to a corn-soybean meal-based diet containing 0, 20, 40, 80, or 160 mg/kg natural ASTA for 4 wk. A total of 540 eggs were collected at the end of the 4-week feeding trial. Sixty fresh eggs were collected and measured for egg quality within 24 h after collection. The other 480 eggs were used in a factorial arrangement with 5 dietary ASTA levels, 4 storage times, and 2 storage temperatures. During the 8-week storage period at 4°C and 25°C, egg quality measurements were performed every 2 wk on 12 eggs per treatment. No significant effects (P > 0.05) on yolk index, yolk pH, Haugh units, weight loss, or eggshell strength were observed with increasing concentrations of dietary ASTA. Yolk color darkened linearly with increasing dose of ASTA (P less then 0.05). During storage of eggs, yolk index and Haugh units decreased significantly (P less then 0.05), whereas yolk pH and weight loss increased (P less then 0.05). An interaction was observed between dietary ASTA level and storage time on yolk index, yolk color, and Haugh units (P less then 0.05). These results demonstrated that dietary ASTA from H. pluvialis delayed the decrease in yolk index and yolk color during storage at 4°C and 25°C. Therefore, we speculate that there may be a combined effect of dietary ASTA level and storage time on egg internal quality; this information may provide additional options by which to extend the storage time of eggs.Growth performance, nutrient digestibility, intestinal health, and endogenous enzyme secretion responses to dietary α-amylase supplementation during 4 growth phases of broiler chickens fed corn-soybean meal-based diets were evaluated in the present study. A total of 1,136 male broiler chicks were assigned at day 0 after hatching to 8 treatments in a 2 × 4 factorial arrangement. There were 2 dietary levels of α-amylase supplementation of 0 or 80 kilo-Novo alpha amylase units per kg diet and 4 posthatching growth phases of day 0 to 11, day 11 to 21, day 21 to 42, or day 42 to 56 in a randomized complete block design. Each treatment comprised 8 replicate pens, with either 25 (day 0-11), 20 (day 11-21), 16 (day 21-42), or 10 (day 42-56) birds per pen. Body weight gain and feed efficiency of birds improved (P less then 0.01) with α-amylase supplementation. There were main effects of α-amylase, growth phase, and interaction (P less then 0.01) on apparent ileal digestibility (AID) of starch. This ranged from 0.8% during day 11 to 21 to 2.8% during day 0 to 11 after hatching. Cyclopamine The total tract retention of starch increased (P less then 0.05) with amylase supplementation but was not different across growth phases. Amylase supplementation increased (P less then 0.05) AID of gross energy, AME (kcal/kg), and AMEn (kcal/kg). Villus height in the jejunal tissue was increased (P less then 0.01) by α-amylase supplementation. During day 11 to 21 after hatching, the viscosity of jejunal digesta and pancreatic amylase activity increased (P less then 0.01) with amylase supplementation. In conclusion, dietary amylase supplementation improved growth performance, apparent nutrient digestibility, and digestive enzyme activity of broiler chickens fed a corn-soybean diet. The study indicates that the growth phase of birds may affect response to exogenous amylase.The effects of formulating broiler diets that contain sources of either rapidly or slowly digestible protein and 2 different dietary fiber sources on growth performance were studied in broilers chickens from 0 to 36 d of age. A total of 1,920 one-day-old, male Ross 708 broiler chickens were randomly allocated and housed in 48 floor pens (40 birds/pen) to one of 4 dietary treatments. Birds were allotted according to a completely randomized block design using a factorial arrangement of treatments with 2 protein digestion rates (rapidly or slowly) and 2 dietary fiber sources [3% oat hulls (OH) or 3% sugar beet pulp (SBP)] from 0 to 36 d of age. All diets were formulated to be isocaloric and isonitrogenous. The pen was the experimental unit for all variables studied (12 replicates/treatment). Data were analyzed using the MIXED procedure of SAS, and the model included the main effects of the protein digestion rate, dietary fiber source, and their interaction. There were 3 experimental feeding phases; starter (fromstion rates to reduce the FCR.Reducing dietary CP for broiler chickens below a certain threshold results in decreased growth, even when the supply of essential amino acids and glycine equivalent (Glyequi) is adequate, probably because other nonessential amino acids (neAA) are growth-limiting. Nonprotein nitrogen (NPN) might be used for the synthesis of neAA. Therefore, the effects of specific neAA and ammonium chloride (NH4Cl) supplementation on the growth and N-excretion characteristics of broiler chickens were investigated. Nine male Ross 308 broiler chickens were kept in each of 81 metabolism units from day 7 to 21 and received 1 of 9 diets in 9 replicates in a one-factorial arrangement of treatments. Two diets with different neAA concentrations, except for Glyequi, were mixed resulting in CP levels of 180 (CP180) and 160 (CP160) g/kg. In six other diets, CP160 was supplemented with either l-Ala, l-Pro, l-Asp, a mix of l-Asp and l-Asn·H2O, l-Glu, or a mix of l-Glu and l-Gln to achieve concentrations of the respective neAA as formulated in CP180. In a further diet, NH4Cl was added to CP160 to achieve the CP concentration of CP180. The ADG and gainfeed ratio (GF) from day 7 to 21 were highest at CP180. Reduced neAA concentrations in CP160 decreased ADG and GF. Supplementation of Asp+Asn, Glu, and Glu+Gln to CP160 increased ADG and GF, but not to the level found for CP180. Compared with CP160, addition of Asp increased GF but not ADG. Supplementation of Asp+Asn caused higher ADG and GF than supplementation of Asp alone. The N-utilization efficiency was highest at CP160 and at CP160 supplemented with Ala, Pro, and Glu. Lower N-utilization efficiency was found at CP180 than at CP160, without and with supplemented neAA. The treatment containing NH4Cl presented the lowest ADG, GF, and N-utilization efficiency. These results showed that individual supplementation of Asp+Asn, Glu, and Glu+Gln partly compensates for the growth-reducing effects of very low CP diets. Supplementation of NH4Cl as NPN source is not suitable for broiler chickens.

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