Colecollins8825

Experimental models of cardiovascular diseases largely depend on the genetic background. Subtotal 5/6 nephrectomy (5/6 Nx) is the most frequently used model of chronic kidney disease (CKD) in rodents. However, in mice, cardiovascular consequences of 5/6 Nx are rarely reported in details and comparative results between strains are scarce. The present study detailed and compared the outcomes of 5/6 Nx in the 2 main strains of mice used in cardiovascular and kidney research, 129/Sv and C57BL/6JRj. Twelve weeks after 5/6 Nx, CKD was demonstrated by a significant increase in plasma creatinine in both 129/Sv and C57BL/6JRj male mice. Polyuria and kidney histological lesions were more pronounced in 129/Sv than in C57BL/6JRj mice. Increase in albuminuria was significant in 129/Sv but not in C57BL/6JRj mice. Both strains exhibited an increase in systolic blood pressure after 8 weeks associated with decreases in cardiac systolic and diastolic function. Heart weight increased significantly only in 129/Sv mice. Endothelium-dependent mesenteric artery relaxation to acetylcholine was altered after 5/6 Nx in C57BL/6JRj mice. Marked reduction of endothelium-dependent vasodilation to increased intraluminal flow was demonstrated in both strains after 5/6 Nx. Cardiovascular and kidney consequences of 5/6 Nx were more pronounced in 129/Sv than in C57BL/6JRj mice.Here we present the synthesis of the efficient nanophosphor Sr2Si5N8Eu2+ (D50 = 144 nm) by a simple milling approach, its strong Rayleigh scattering, and its film applications to white LED and silicon solar cell as a downshifting medium. see more The final nanophosphor product showed the quantum efficiency comparable to the bulk phosphor which is, to our knowledge, the highest record of nitride nanophosphors. Especially the nanophosphor showed the more tail emission at the shorter-wavelength side of the emission spectrum and the faster thermal quenching with the more spectral broadening along with the temperature due to Rayleigh scattering. Also the lowering in the excitation spectrum was observed due to lower absorbance. Finally, the nanophosphor-dispersed polyvinyl alcohol (PVA) film was made, and its applications to white LED and silicon solar cell as a downshifting medium demonstrated that it gave the high color rendering property in white LED in spite of still lower luminous efficiency, and it caused the increase in efficiency of silicon solar cell.Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal forms of cancer. One major reason for this is that PDAC quickly metastasizes to other organs, thereby making its treatment difficult. The molecular machinery driving PDAC metastasis is still poorly understood. In this study, we applied an unbiased approach using CRISPR screening to identify genes that strongly regulate invasion (based on an in vitro assessment of their metastatic potential) in PANC-1, a PDAC cell line. Through CRISPR screening, we identified MBNL3 and KANSL2 as strong regulators of invasion in PANC-1 cells. We further validated MBNL3 and KANSL2 as regulators of PANC-1 cell invasion by using the doxycycline-inducible shRNA system. We also showed that MBNL3 and KANSL2 do not affect cell proliferation. Through our efforts, we have established a process to identify genes that regulate cell invasion and can be further investigated as potential targets for therapeutic intervention.As long-distance races have substantially increased in popularity over the last few years, the improvement of training programs has become a matter of concern to runners, coaches and health professionals. Triaxial accelerometers have been proposed as a one of the most accurate tools to evaluate physical activity during free-living conditions. In this study, eighty-eight recreational marathon runners, aged 30-45 years, completed a marathon wearing a GENEActiv accelerometer on their non-dominant wrist. Energy consumed by each runner during the marathon was estimated based on both running speed and accelerometer output data, by applying the previously established GENEActiv cut-points for discriminating the six relative-intensity activity levels. Since accelerometry allowed to perform an individualized estimation of energy consumption, higher interpersonal differences in the number of calories consumed by a runner were observed after applying the accelerometry-based approach as compared to the speed-based method. Therefore, pacing analyses should include information of effort intensity distribution in order to adjust race pacing appropriately to achieve the marathon goal time. Several biomechanical and physiological parameters (maximum oxygen uptake, energy cost of running and running economy) were also inferred from accelerometer output data, which is of great value for coaches and doctors.We use individual-based information on the behavior of wild female Japanese macaques in two consecutive years with different food availability (nut-rich vs. nut-poor) to test effects of dominance rank and nut fruiting on seed dispersal parameters. We predicted that social rank would affect dispersal (1) quantity, (2) quality, (3) species richness, and (4) percentage of berries in the diet in the nut-poor year, while these differences would disappear in the nut-rich year. We found seeds of nine fleshy-fruited plant species in the feces of the monkeys. The frequency of seed occurrence for two plant species (Viburnum dilatatum and Rosa multiflora) showed an interaction between dominance ranks and years; in the nut-poor year V. dilatatum seeds were more abundant among dominant females and R. multiflora among subordinates, while such inter-rank differences disappeared in the nut-rich year. Similarly, the intact ratio of V. dilatatum seeds was lower for dominants in the nut-poor year, while inter-rank variations disappeared in the nut-rich year. Finally, percentage of berries in diet and seed richness showed no inter-annual nor inter-rank variations. Our study highlights that differences in individuals' social rank lead to within-group variation in seed dispersal services and that these differences are dependent on nut availability.