Sandersstorm7544

The aim of this work was to investigate the mechanical behaviour of alginate-based composite particles. Alginate gel beads with entrapped starch were used as the replicates of storage cells of plant tissue. Beads were formulated using different ratios of both ingredients and were produced using two methods, resulting in particles in the macro- and micro-scale size range. Compression tests revealed an effect of bead size on mechanical properties and a dominant role of the alginate on the material properties. Starch was successfully encapsulated as native granules in the beads and once encompassed, it suffered restricted swelling, up to 45 % of its original size, after undergoing heating. Force versus displacement data were fitted to both an empirical and the Hertz model and Young's modulus was found to increase only with heated starch inclusions. Microscopy was deemed crucial for the interpretation of mechanical measurements.A novel image segmentation methodology combined with optical microscopy observation was developed for qualifying starch swelling. Starch granules in the micrograph were successfully segmented based on high-precision edges extraction achieved by Canny edge detection together with mathematical morphology operation. Granules were automatically identified by computer vision and characterized by giving quantifiable area of these granules. The evolved swelling process could be generally divided into two phases. During the first phase, starch granules were only swollen up by 2.56 %, which is hard to be identified by conventional naked eye. During the following narrow temperature interval (60-66 ℃), these starch granules were detected to swell up significantly by 9.08 %. Through the granule area variable, swelling capacity was high-throughput characterized, which allows for the whole evaluation to be completed within a couple of minutes. The proposed methodology showed a high accuracy and potential as a novel technique for characterizing gelatinization.Natural polysaccharides have attracted considerable interests due to diverse biological activities. Succinoglycan is an extracellular polysaccharide produced by most Agrobacterium strains. Here, we confirmed riclin was a typical succinoglycan by NMR and methylation analysis, and investigated the antitumor effects of riclin in sarcoma 180 tumor-bearing mice. The results showed that riclin inhibited the tumor growth significantly as well as cyclophosphamide (CTX). While CTX caused serious damage to spleen structure, riclin increased the spleen index and promoted lymphocytes proliferation in peripheral blood, spleen and lymph nodes. Riclin decreased splenocytes apoptosis as evidenced by alterations of B-cell lymphoma-2 family proteins and Cleaved Caspase-3 protein. Moreover, 1H nuclear magnetic resonance (NMR)-based metabolomics analysis revealed that riclin partially altered the metabolic profiles of splenocytes. In conclusion, riclin is a succinoglycan that performed strong immunogenicity and suppressed sarcoma growth in mice. Succinoglycan riclin could be a potential antitumor agent for functional food and pharmaceutical purpose.An Ultra-lightweight cellulose-based foam material (ULW-CFM) reinforced with silicasol was prepared by foam forming technology. The sodium dodecylbenzene sulfonate (SDBS) was a suitable foaming agent, creating stable bubbles for ULW-CFM with silicasol. The modulus of elasticity of ULW-CFM modified by silicasol was enhanced from 14.8 kPa to 83.32 kPa, and the yield strength was improved from 1.6 kPa to 4.36 kPa, respectively. The FTIR analysis indicated that the silicasol was deposited inside the material by SiOSi bonds and might has dehydration condensation reaction with cellulose. The thermostability of this material was also enhanced by silicasol. The weight loss of ULW-CFM with silicasol was less pronounced (60.36 %) than the one without (81.12 %). Owing to three dimensionally dispersed fiber structure, ultralightweight, high porosity, the sound absorption coefficient of ULW-CFM with silicasol reached 0.65 above 5000 Hz. Results show that ULW-CFM modified by silicasol has a promising application as a degradable sound absorbing material for dealing with noise.Effect and working mechanism of temperature and pH on encapsulation and release of β-carotene from octenylsuccinated-oat-β-glucan-micelles (OSβG-Ms) were investigated. The stability and solubility of β-carotene, and changes in surface hydrophilicity, core hydrophobicity, and size of β-carotene-loaded-OSβG-Ms were determined. When exposed to temperature (25-45 °C) and pH (4.5-8.5), β-carotene solubilization changed in parabolic manners. Size and absolute zeta-potential of β-carotene-loaded-OSβG-Ms decreased with temperature, while they gave parabolic changing patterns with pH. Those results were ascribed to their hydrophilicity, hydrophobicity, and core/shell compactness via regulating molecule mobility, orientation, and interactions by temperature/pH. The higher temperature concluded with higher β-carotene release, while a U-shaped release profile was observed with pH. Besides its diffusion, erosion-induced shrinking and collapsing of OSβG-Ms favored β-carotene release at pH 1.2-4.5, which was replaced by swelling-induced structural-relaxation at pH 6.8-8.5. The results were favourable in controlling the behavior of β-carotene-loaded-OSβG-Ms by selectively applying environmental parameters.The aim of this work was to develop a comprehensive workflow to elucidate molecular features of artichoke pectic oligosaccharides (POS) contributing to high potential prebiotic activity. First, obtainment of artichoke POS by Pectinex® Ultra-Olio was optimised using an artificial neural network. Under optimal conditions (pH 6.86; 1.5 h; enzyme dose 520.5 U/g pectin) POS yield was 624 mg/g pectin. Oligosaccharide structures (Mw less then 1.3 kDa) were characterised by MALDI-TOF-MS. Then, conformational analysis of glycosidic bonds was performed by replica exchange molecular dynamics simulations and interaction mechanisms between POS and several microbial glycosidases were proposed by molecular modelling. Chemical information was integrated in virtual simulations of colonic fermentation. Highest hydrolysis rate was obtained for GalA-Rha-GalA trisaccharide, while the presence of partial negative charges and high radius of gyration enhance short chain fatty acid formation in distal colon. click here Established structure-activity relationships could help the rational design of prebiotics and clinical trials.