Dissingfitzsimmons9907

This study aimed to assess the potential environmental benefits of implementing low-cost digesters to valorize agro-industrial waste in the non-centrifugal cane sugar (NCS) sector. Two scenarios were considered i) the current scenario in which organic waste and wastewater were burned outdoor and discharged into a water body, respectively; ii) the anaerobic digestion (AD) scenario, in which low-cost biodigesters were used for organic waste and wastewater treatment on-site. Results showed that low-cost digesters were a sustainable alternative to mitigate environmental impacts, especially those associated with water source pollution. Indeed, in the AD scenario, the environmental impact categories of Freshwater Eutrophication and Marine Eutrophication showed a decrease of 87.6% and 99.4%, respectively, compared to the current scenario. Thus, by treating organic waste and wastewater on-site while producing bioproducts (i.e. biofuel and biofertilizer), low-cost digesters could contribute to boosting the circular bioeconomy in the NCS production sector.Utilization of glycerol, a biodiesel byproduct, has not been well explored. In the present study, glycerol and the other carbon sources were studied for cometabolism of dibenzothiophene (DBT), a model chemical commonly used in bioremediation studies, by Paraburkholderia sp. Ziritaxestat order C3. This study showed a direct association between rhamnolipids (RLs) biosynthesis and DBT biodegradation induced by different carbon sources in a Paraburkholderia specie. Glycerol can induce the strain C3 produce at least four RLs. The RL precursor is mainly derived from the fatty acid synthesis (FAS II) and β-oxidation pathway. The genome contained two (fabF and fabG) and four (fadA, fadE, fadB and echA) genes involved in FAS II and β-oxidation, respectively. The genome also carried the rhlA and rhlB genes involved in rhamnosyltransferase for RL biosynthesis and two DBT dioxygenase genes (nahAc and catA). The findings suggest a viable approach of using the biodiesel byproduct glycerol to remediate contaminated environments.Aerobic denitrifying bacteria were widely reported in different nitrogen polluted aquatic ecosystem. However, the aerobic denitrification characteristics of actinomycete were not well understood. Here, the actinomycete strain XD-11-6-2 was isolated from reservoir and identified as Streptomyces sp. XD-11-6-2 by DNA sequencing. Strain XD-11-6-2 removed 90.34% of total organic carbon and 93.66% of total nitrogen under aerobic condition. A total of 77.87% of nitrogen was removed as a gaseous product, and 15.67% of nitrogen was converted into biomass. Biolog combined with network model indicated that strain XD-11-6-2 could use six types of carbon sources, and exhibit outstanding capacity to metabolize diverse carbon sources. Moreover, the highest nitrate and total nitrogen removal efficiencies of raw water were 72.29% and 74.86%, respectively. In general, these results provide new insights to understand the potential of actinomycetes in treating micro-polluted water.O-linked N-acetylglucosamine (O-GlcNAc) is protein modification that is emerging as a regulator of diverse aspects of cellular physiology. Aberrant O-GlcNAcylation has been linked to several diseases, spurring the creation of methods to detect and perturb the activity of the two enzymes that govern this modification - O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). Here we summarize assays used for these two enzymes. We also detail the latest structure-guided development of inhibitors of these two enzymes and touch on selected reports that underscore the utility of inhibitors as tools for uncovering the diverse roles of O-GlcNAc in cell function. Finally, we summarize recent reports on the potential therapeutic benefits of antagonizing these enzymes and comment on outstanding challenges within the field.

The rate of caesarean section (CS) is increasing globally. The nulliparous, term, singleton, vertex presentation, spontaneously labouring woman (Robson Group 1/RG1) is considered low risk for CS. It has been hypothesized that more CS occur at nighttime or at weekends due to doctor fatigue. The European Working Time Directive (EWTD) was implemented in our institution in 2013 to limit doctor working hours, which aimed at reducing fatigue but arguably fractures continuity of care. This study aimed to determine the effect of nocturnal hours and weekend on-call as well as the implementation of EWTD on our RG1 CS rates.

This was a population-based study in a tertiary referral centre from 2008-2017. The inclusion criteria for our study were limited to RG1. Data were analysed from an established clinical database, including mode and time of delivery. Descriptive statistics are presented as number and percent for categorical variables. Relative frequencies were tested using chi-squared test. All statistical analysrelation to simulation training could increase the OVD rate and reduce the CS rate.

matrix metalloproteinases including matrix metalloproteinase-2 play a key role in endometrial extra cellular matrix breakdown in endometriosis. Aberrant expression of matrix metalloproteinase-2 has been reported in eutopic and ectopic endometrial tissue of endometriosis patients so altered expression of matrix metalloproteinase-2 due to polymorphisms may lead to establishment and progression of endometriosis. In this study the association between -735 C/T (rs2285053) and -1575 G/A (rs243866) variants of matrix metalloproteinase-2 gene with presence of endometriosis in an Iranian population were investigated for the first time.

A case-control association study was conducted to investigate the role of MMP-2-735 C/T and _1575 G/A variants in development of endometriosis. Polymerase chain reaction-restriction fragment length polymorphism method was used to determine genotype frequencies of these variants in 100 endometriosis patients and 200 normal samples. Total genomic DNA was extracted from blood samples ae-2 gene can increase the risk of endometriosis in Iranian women.

In conclusion, existence of rs243866 variant in promoter region of matrix metalloproteinase-2 gene can increase the risk of endometriosis in Iranian women.