Sosaspence3978

To determine the relative expression of long non-coding small nucleolar RNA host gene 7 (lncRNA-SNHG7) in fibrotic liver and hepatic stellate cells, and investigate the biological effects and mechanisms of SNHG7 on liver fibrosis.

Liver fibrosis model of mice was established, primary hepatic stellate cells (HSCs) were cultured from normal mice and induced to activate by TGF-β. Cell viability, proliferation, and autophagy were detected with MTT, BrdU, and MDC stain, respectively. Liver tissue stained with Masson and Sirius Red. The interaction between SNHG7 and miR-29b was investigated by immunoprecipitation, RNA pull-down and Dual-luciferase reporter gene assay. The effects of SNHG7 on the expression of miR-29b, DNMT3A and liver fibrosis related factors were detected in vitro or in vivo transfection experiments.

SNHG7 was signally increased in liver tissue and HSCs of liver fibrosis model of mice, and inhibition of SNHG7 expression in liver fibrosis mice can reduce liver fibrosis. We also found that SNHG7 could bind to miR-29b in HSCs and inhibit the expression of miR-29b. In TGF-β-stimulated normal HSCs, knockdown of SNHG7 expression after shSNHG7 transfection could restrain DNMT3A and HSCs activation factors α-SMA, Collα1 and autophagy-related factors LC3I/II, Beclin1. However, this shSNHG7 effect was reversed by the inhibition of miR-29b.

Inhibition of lncRNA-SNHG7 can inhibit liver fibrosis. This is partly due to SNHG7 acts as a competitive endogenous RNA (ceRNA) to affect the expression of DNMT3A, a downstream target gene of miR-29b, by binding to miR-29b, thereby affecting the activation, autophagy and proliferation of HSCs.

Inhibition of lncRNA-SNHG7 can inhibit liver fibrosis. This is partly due to SNHG7 acts as a competitive endogenous RNA (ceRNA) to affect the expression of DNMT3A, a downstream target gene of miR-29b, by binding to miR-29b, thereby affecting the activation, autophagy and proliferation of HSCs.Cholangiocarcinoma (CCA) is the second most common primary liver cancer, characterized by a poor prognosis and resistance to chemotherapeutics. The progressive increase in CCA incidence and mortality registered worldwide in the last two decades and the need to clarify various aspects of clinical management have prompted the Italian Association for the Study of the Liver (AISF) to commission the drafting of dedicated guidelines in collaboration with a group of Italian scientific societies. These guidelines have been formulated in accordance with the Italian National Institute of Health indications and developed by following the GRADE method and related advancements.

The aim of this cross-sectional observational study was to measure habitual viewing distance with smartphones in individuals of different ages, and to identify factors influencing viewing distance.

Gender, reading position, forearm length, ametropia, correction modality, and near vision quality were investigated as factors affecting viewing distance. Participants were asked to read a typical text message on their own smartphone, while sitting and standing. A total of 233 subjects (129 females and 104 males), aged from 16 to 90 years old, were included and divided in two groups 131 non-presbyopes (median 21.1, range 16 - 39), and 102 presbyopes (median 54.5, range 42 - 90).

Mean viewing distance was 36.1 ± 7.2 cm while sitting, and 37.4 ± 6.8 cm while standing (P < 0.05), and 36.8 +/- 6.6 cm overall. The average viewing distance was 35.0 ± 6.4 cm in non-presbyopes, and 39.0 ± 6.1 cm in presbyopes (P < 0.05). The average viewing distance was 34.7 ± 6.2 cm in females, and 38.2 ± 6.3 cm in males (P &lved in both age groups, without a statistically significant correlation with other assessed factors as reading position, ametropia, correction modality, and near vision quality.The Surgivisio system is a new generation of intraoperative 3D imaging, used in our institution for pedicle insertion in minimally invasive spine surgery since January 2018. The purpose of this technical note is to describe the Surgivisio system, its advantages and its limitations, in percutaneous spinal surgery. Results of the first year of use were analyzed, in a series of 29 patients, to evaluate accuracy of pedicle screw insertion, operative time and radiation exposure. On the Heary and Gertzbein classifications, 95.5% of pedicle screw placements (107/112) were rated as acceptable. Mean operative time was 29.3min per vertebra; mean radiation exposure per vertebra was 0.61 mSv. The Surgivisio system is an effective navigation tool for pedicle screw insertion in minimally invasive spinal surgery, with acceptable radiation exposure and operative time for each navigated vertebra. Selleckchem 3PO LEVEL OF EVIDENCE II, prospective cohort study.

Some clinical situations, such as great sagittal imbalance, high-grade isthmic spondylolisthesis or sacral malunion could require a sacral osteotomy to decrease pelvic parameters, horizontalize the sacrum or correct sacral malunion. Here is described a novel technique to perform a sacral osteotomy to decrease pelvic parameters with a lumbo-pelvic construct, with first a sacral slope decrease, then a pelvic tilt decrease.

Simulations have been performed using tridimensional reconstructions of the lumbar spine and pelvis, made from CT-scan images of a healthy individual. A cadaveric study has then been performed.

3D modeling exhibited linear relationship between osteotomy angle and pelvic incidence correction, through multiple simulations with 1° increment. Cadaveric study demonstrated feasibility.

This preliminary work shows that this technique is efficient to decrease pelvic parameters. A linear relationship has been exhibited between osteotomy angle and PI decrease, as per the following formula osteotomy angle=PI change/0.84.

This preliminary work shows that this technique is efficient to decrease pelvic parameters. A linear relationship has been exhibited between osteotomy angle and PI decrease, as per the following formula osteotomy angle=PI change/0.84.

Wilms' tumor (WT) is the most common type of malignant kidney tumor in children. Three-dimensional reconstructions can be performed pre-operatively to help surgeons in the planning phase.

The main objective of this study was to determine the variability of WT segmentation and 3D reconstruction. The secondary objectives were to assess the usefulness of these 3D reconstructions in the surgical planning phase and in the selection of patients for nephron-sparing surgery (NSS).

14 scans from 12 patients were manually or semi-automatically segmented by 2 teams using 3D Slicer software. Inter-individual variability of 3D reconstructions was measured based on the Dice index. The utility of 3D reconstructions for the surgical planning was evaluated by 4 pediatric surgeons using a 5-point Likert scale. The possibility of undertaking NSS was evaluated according to the criteria defined in the Umbrella SIOP-RTSG 2016 protocol.

Segmentation of the WT, healthy kidney, pathological kidney, arterial and venous vascularization could be performed for all of the patients in this study.