Hamiltonkronborg3294

Our approach opens up routes for coherently manipulating the interactions and properties of two-dimensional and other quantum materials using light.Stomatopod crustaceans possess some of the most complex animal visual systems, including at least 16 spectrally distinct types of photoreceptive units (e.g., assemblages of photoreceptor cells). Here we fully characterize the set of opsin genes expressed in retinal tissues and determine expression patterns of each in the stomatopod Neogonodactylus oerstedii Using a combination of transcriptome and RACE sequencing, we identified 33 opsin transcripts expressed in each N. oerstedii eye, which are predicted to form 20 long-wavelength-sensitive, 10 middle-wavelength-sensitive, and three UV-sensitive visual pigments. Observed expression patterns of these 33 transcripts were highly unusual in five respects 1) All long-wavelength and short/middle-wavelength photoreceptive units expressed multiple opsins, while UV photoreceptor cells expressed single opsins; 2) most of the long-wavelength photoreceptive units expressed at least one middle-wavelength-sensitive opsin transcript; 3) the photoreceptors involved in spatial, motion, and polarization vision expressed more transcripts than those involved in color vision; 4) there is a unique opsin transcript that is expressed in all eight of the photoreceptive units devoted to color vision; and 5) expression patterns in the peripheral hemispheres of the eyes suggest visual specializations not previously recognized in stomatopods. Elucidating the expression patterns of all opsin transcripts expressed in the N. oerstedii retina reveals the potential for previously undocumented functional diversity in the already complex stomatopod eye and is a first step toward understanding the functional significance of the unusual abundance of opsins found in many arthropod species' visual systems.The bacterial flagellum is an amazing nanomachine. Understanding how such complex structures arose is crucial to our understanding of cellular evolution. We and others recently reported that in several Gammaproteobacterial species, a relic subcomplex comprising the decorated P and L rings persists in the outer membrane after flagellum disassembly. Imaging nine additional species with cryo-electron tomography, here, we show that this subcomplex persists after flagellum disassembly in other phyla as well. Bioinformatic analyses fail to show evidence of any recent horizontal transfers of the P- and L-ring genes, suggesting that this subcomplex and its persistence is an ancient and conserved feature of the flagellar motor. We hypothesize that one function of the P and L rings is to seal the outer membrane after motor disassembly. Copyright © 2020 the Author(s). Published by PNAS.Rapid methods for diagnosis of bacterial infections are urgently needed to reduce inappropriate use of antibiotics, which contributes to antimicrobial resistance. In many rapid diagnostic methods, DNA oligonucleotide probes, attached to a surface, bind to specific nucleotide sequences in the DNA of a target pathogen. Typically, each probe binds to a single target sequence; i.e., target-probe binding is monovalent. Here we show using computer simulations that the detection sensitivity and specificity can be improved by designing probes that bind multivalently to the entire length of the pathogen genomic DNA, such that a given probe binds to multiple sites along the target DNA. Our results suggest that multivalent targeting of long pieces of genomic DNA can allow highly sensitive and selective binding of the target DNA, even if competing DNA in the sample also contains binding sites for the same probe sequences. Our results are robust to mild fragmentation of the bacterial genome. Our conclusions may also be relevant for DNA detection in other fields, such as disease diagnostics more broadly, environmental management, and food safety. Copyright © 2020 the Author(s). Published by PNAS.Characterization and prediction of the "flowability" of powders are of paramount importance in many industries. Selleck ONO-7475 However, our understanding of the flow of powders like cement or flour is sparse compared to the flow of coarse, granular media like sand. The main difficulty arises because of the presence of adhesive forces between the grains, preventing smooth and continuous flows. Several tests are used in industrial contexts to probe and quantify the "flowability" of powders. However, they remain empirical and would benefit from a detailed study of the physics controlling flow dynamics. Here, we attempt to fill the gap by performing intensive discrete numerical simulations of cohesive grains flowing down an inclined plane. We show that, contrary to what is commonly perceived, the cohesive nature of the flow is not entirely controlled by the interparticle adhesion, but that stiffness and inelasticity of the grains also play a significant role. For the same adhesion, stiffer and less dissipative grains yield a less cohesive flow. This observation is rationalized by introducing the concept of a dynamic, "effective" adhesive force, a single parameter, which combines the effects of adhesion, elasticity, and dissipation. Based on this concept, a rheological description of the flow is proposed for the cohesive grains. Our results elucidate the physics controlling the flow of cohesive granular materials, which may help in designing new approaches to characterize the "flowability" of powders. Copyright © 2020 the Author(s). Published by PNAS.Multi-cellular organisms use mitogens to regulate cell proliferation, yet how fluctuating mitogenic signals are converted into proliferation-quiescence decisions is poorly understood. Here we combine live-cell imaging with temporally controlled perturbations to determine the timescale and mechanisms underlying the system. Contrary to the textbook model that cells only sense mitogen availability in G1, we find that mitogenic signaling is temporally integrated throughout the entire mother cell cycle, and even a one-hour lapse in mitogen signaling can influence cell proliferation over 12 hours later. Protein translation rates serve as the integrator that proportionally converts mitogen history into corresponding levels of Cyclin D in G2 phase of the mother cell, which controls the proliferation-quiescence decision in daughter cells, and thereby couples protein production with cell proliferation. Copyright © 2020, American Association for the Advancement of Science.