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This study conducted two experiments to evaluate the effects of two cheap adsorbents, including bentofeed (a commercial name of bentonite) and Persian melon peel biochar (PMPB) on the decolorization of water contaminated by methylene blue (MB) and ruminal fermentation pattern. The decolorization efficiency of bentofeed and PMPB at three levels of 0, 4, and 8 mg per 10 ml of 0, 3, 6, and 9 mg/L MB solutions mg/L after 3 and 24 h of incubation was evaluated by its absorbance at 660 nm. At all dye concentrations, PMPB, and bentofeed showed high potential in removing MB from water with an efficiency of 60%-99.5%. In both incubation times, the addition of 8 mg bentofeed had the highest effect on the removal efficiency when the dye concentration was 6 or 9 mg/L. However, the removal efficiency was declined with increasing MB concentration (p <0.05). Experiment two evaluated the effects of various levels (same as experiment one) of MB, bentofeed, and PMPB on in vitro gas production (GP) and volatile fatty aci bentofeed has the higher adsorption property of MB, compared to that of the PMPB.The present study aimed to assess the effect of tissue hypoxia induced by sodium cyanide (NaCN) on male mice fertility and the protective role of ethyl pyruvate (EP). A number of 30 adult mice were assigned to three groups 1) a control group, 2) a treatment group treated with 2 mg/kg of NaCN, and 3) a treatment group treated with 2 mg/kg of NaCN, along with 40 mg/kg EP (NaCN+EP). After 35 days, animals were anesthetized and serum, sperm, and tissue samples were taken. The results demonstrated a significant decrease in sperm quality, reproduction potency, and anti-oxidant potential, as well as an increase in lipid peroxidation in the NaCN group (p <0.05). Moreover, the use of EP effectively restrained the disastrous effects of tissue hypoxia. It can be concluded that EP can moderate the complications resulting from tissue-hypoxia that is related to testes parameters.The present study investigated the fine structure of amphids and phasmids, cuticle, muscles, and digestive tracts of Toxocara canis using optical and electron microscopy, hematoxylin-eosin (H&E) staining, and other specific stains. A number of 38 adult T.canis worms were obtained from the animal shelter of Urmia, and their small intestines were fixated in acidified formal alcohol and 10% formalin solutions. The anterior and posterior parts of male and female T.canis worms were prepared and cut at a thickness of 4-5 μm according to the conventional method in the histological laboratory. The samples were then stained using H&E and specific periodic acid-Schiff, Masson's trichrome, and Orcein staining. The structure of amphid (anterior), phasmid (posterior), cuticle, muscles, and digestive tracts of male and female worms were studied under light microscopy. Basal, intermediate, cortex, and cuticle surface coating of the parasite were visible. Alae were also observed as the thickeni core of these protrusions.Fish represents one of the major sources of animal proteins, and different species of fish are susceptible to infections with parasites which cause severe tissue damage and cell destruction of the infected organ. Therefore, in 2019, this parasitological study was conducted to assess the helminth parasites infecting the African sharptooth catfish Clarias gariepinus that were collected from Lake Manzala, Egypt. Only nematode parasite was reported as a prevalent infection from the fish stomach with an infection rate of 7.5%. Depending on the seasonal prevalence, the extent of the infection was analyzed. LY303366 cost It was indicated that parasite infection was only reported as 15% in the winter season. Morphological and morphometric analyses of the present parasite species revealed that it possesses all the characteristics of the Camallanus genus, whereas it is closely related to Camallanus polypteri described previously. It is characterized by the presence of a buccal capsule with longitudinal internal ridges, some of which are very short and ranged from 8-14 in males and 8-9 in females. The esophagus consisted of muscular and glandular portions, the middle position of the excretory pore to the muscular esophagus, the anterior location of deirids to the nerve ring, posterior end of males with two unequal spicules and caudal papillae; nonetheless, it is smooth and straight in females. In addition, some morphology and measurement differences for the different body parts were identified with other Camallanus species. Therefore, the present study can provide a full morphologically re-description of Camallanus polypteri with a new geographical location in the Egyptian freshwater.Hyalomma spp. is responsible for the transmission of protozoan, bacterial, rickettsial, and viral diseases and causes huge economic loss to the livestock industry.Recently, there is a wide number of promising attempts to evaluate and use herbal preparations for ticks control.This study aimed to evaluate the acaricidal activity of aqueous and ethanol extracts of Colchicum autumnale (C. autumnale) rhizome and leaf against the Hyalomma spp. in vitro. The acaricidal activities of the Colchicum leaf aqueous (CLA), Colchicum leaf ethanolic (CLE), Colchicum rhizome aqueous (CRA), and Colchicum rhizome ethanolic (CRE) extracts were evaluated at concentrations of 50, 100, and 150 mg/ml and controls (distilled water and Cypermethrin) following 0.25, 0.5, 0.75, and 1 h of exposure. It is worth mentioning that the spraying method was used in these experiments. Data were analyzed through GraphPad Prism 5 software. In addition, the chemical composition of aqueous leaf extract was analyzed using gas chromatography-mass spectrometry (GC–MS). The carbamodithioic acid (30.04%) was the major chemical constituent identified. Based on the results, CLA, CLE, CRA, and CRE extracts had an acaricidal effect; however, this effect was more potent in CLE. The CLE extract showed a 100% mortality rate at 50, 100, and 150 mg/ml concentrations and 1 h of exposure. The effectiveness of CRA on the Hyalomma spp. was very low. The median lethal concentration (LC50) values were obtained at 100 mg/ml. The results indicated that C. autumnaleleaves contained potent acaricidal ingredients and might provide new acaricidal compounds for the effective control of Hyalomma spp. However, further studies are required to evaluate the efficacy of C. autumnale in vivo.

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