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We examined the antimicrobial susceptibility of 848 Escherichia coli isolates from 237 feces samples of wild sika deer (Cervus nippon) captured between 2016 and 2019 in 39 of the 47 prefectures of Japan. Five of the 237 wild sika deer (2.1%) carried E. coli with resistance to at least one antimicrobial, and all the resistant isolates showed resistance to tetracycline. The resistant isolates contained antimicrobial resistance genes that were similar to those in E. coli derived from humans and farm animals. Although wild sika deer are not currently likely to be a source for the transmission of antimicrobial resistance in Japan, they can potentially mediate antimicrobial resistance spread by coming into contact with humans, animals, and their surroundings.Feline noroviruses (FNoVs) are potential clinical pathogens in cats. To perform an epidemiological study of FNoV infection, it is necessary to develop a simple and effective method for virus detection. We investigated whether a commercial human NoV quantitative RT-PCR kit for the detection of human NoVs used in medical practice can be applied for FNoV detection. This kit was capable of detecting the FNoV gene regardless of the genogroup (GIV and GVI) in experimental and field samples. Based on the above findings, it is possible to detect FNoVs using human NoV tests. The relationship between FNoV infection and gastroenteritis in cats may be clarified by applying these methods to an epidemiological survey of FNoVs.This report describes the cases of two Miniature Dachshunds who were suspected to have immune-mediated hemolytic anemia (IMHA) and were treated with immunosuppressive therapy. However, progression of anemia, increases in C-reactive protein (CRP) and total-bilirubin (T-Bil) levels, splenomegaly, transition to nonregenerative anemia, and thrombocytopenia occurred after the treatment. Splenectomy and bone-marrow aspirations were performed subsequently. selleck Both dogs were diagnosed with hemophagocytic syndrome (HPS) associated with IMHA. Unfortunately, they died 9 and 6 days later. These findings indicate that some cases of refractory IMHA have the pathogenicity of HPS. HPS should be included as a differential diagnosis of refractory IMHA concurrent with thrombocytopenia. Continuously elevated CRP and T-Bil levels may be helpful indicators in the detection of HPS associated with IMHA.An adult Red-crowned crane in captivity that had a displaced compound fracture of the middle upper beak caused by an accident was treated by using Type I-a external skeletal fixator (ESF). The ESF that was equipped with a unilateral epoxy putty fixator and with five half-pins was fixed on the premaxilla bone. The crane with the ESF on the beak was able to feed smoothly by itself. The ESF was removed 49 days after the first surgery. Beak malocclusion, which was observed in the latter half period of fixing, spontaneously improved within one month after removal of the ESF. This is the first successful case of repair of an upper beak fracture in a Red-crowned crane.One of the ultimate goals of population genetics is to theoretically describe the behavior of allele frequency. Diffusion theory has been commonly used for this purpose mainly in one-locus one-population models, although it is not easy to handle diffusion theory in models with multiple loci or with multiple populations. This review introduces several successful cases, where multi-dimensional diffusion equations contributed to addressing evolutionary questions, thereby demonstrating its strong potential in population genetics.Ubiquitination is a reversible post-translational modification in which ubiquitin chains are conjugated to target proteins to modulate protein function. The type of ubiquitin chain determines the mode of protein regulation. It has been shown that ubiquitin chains are formed via one of seven Lys residues in ubiquitin, and several types of ubiquitin chains are found in cells. We identified a new type of linear ubiquitin chain linked through the N-terminal Met of ubiquitin and assembled by the linear ubiquitin chain assembly complex (LUBAC), which is specific for linear chains. link2 The discovery of linear ubiquitin chains and LUBAC is considered as a paradigm shift in ubiquitin research because linear ubiquitination is exclusive to animals, despite the existence of ubiquitination throughout eukaryotic kingdoms. Linear ubiquitination plays a critical role in immune signaling and cell death regulation. Dysregulation of LUBAC-mediated linear ubiquitination underlies various human diseases, including autoinflammation, autoimmunity, infection, and malignant tumors. link3 This review summarizes the current status of linear ubiquitination research.Understandings of turbulent plasma have been developed along with nuclear fusion research for more than a half century. Long international research has produced discoveries concerning turbulent plasma that allow us to notice the hidden nature and physics questions that could contribute to other scientific fields and the development of technologies. Guiding concepts have been established up to now that stimulate investigations on turbulent plasma. Research based on concepts concerning symmetry breaking and global linkage requires observing the entire field of plasma turbulence for an ultimate understanding of plasma. This article reviews the achievements as well as contemporary problems regarding turbulence experiments associated with strongly magnetized plasmas in the last and present century, and introduces forthcoming experimental issues, including new diagnostics and physics-oriented devices related to plasma turbulence.The aim was to investigate effects of compliance and radiant emittance on cuspal deflection. A mesio-occluso-distal cavity was replicated using a flowable composite and steel rods with different diameters. To investigate the effect of cuspal compliance on deflection, the replicated tooth and extracted human tooth cavities were bulk-filled with a micro-hybrid composite. The replicated cavities with 2.0-mm-diameter steel rods were restored with bulk-filling of the micro-hybrid or a nano bulk-fill composite and photo-cured with four protocols. The cuspal deflection was the highest in the replicated tooth without steel rod, followed by with 1.0-, 1.5-, and 2.0-mm steel rods, and significantly lower in the human tooth. Cuspal deflections showed no significant differences among light-curing protocols, and that in the micro-hybrid composite were higher than the nano bulk-fill composite. To reduce cuspal deflection of a high compliance cavity, selecting the proper composite based on physical properties is more important than light-curing protocol.Yttria doped ZrO2 was deposited using an acidic zinc phosphatizing solution and the hydrothermal treatment. The coating was analyzed using a field emission-scanning electron microscope (FE-SEM), X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDX) and X-ray photoelectron spectroscopy (XPS). A piston on three balls (ISO 6872) was used for the measurement of biaxial flexural strength. MC3T3-E1 cells attachment was evaluated by SEM, and cell proliferation were assessed using MTS assay™. SEM images confirmed that the zinc phosphate coating layer was successfully prepared and fully covered the surface. The measured adhesive strength of the coating was 79.11 MPa. In vitro cell study indicated that the coated sample had better cell morphology and proliferation. XRD and EDS analysis revealed that the crystalline coating structure indexed as zinc phosphate (hopeite) and the substrate was assigned as zirconia. The flexural strength test showed that the strength of zirconia before and after hydrothermal treatment was not affected.The objective of this study was to clarify the fatigue behavior of hollow yttria-stabilized tetragonal zirconia polycrystals (Y-TZP) specimens assuming its use for two-piece implants. The fatigue properties of a solid specimen (which simulated a one-piece implant) and 3 types of hollow specimens (which simulated two-piece implants) were evaluated. Specimens were either solid with a diameter of 4.0 mm (S) or hollow with an inner diameter of 3.0 mm and outer diameters of 4.0 mm (H0.5), 4.5 mm (H0.75), or 5.0 mm (H1.0). For each group, 25 specimens were prepared followed by blast and acid etch treatment. Static fracture and cyclic fatigue tests were conducted by modifying the methods provided in ISO6872. Fracture modes were determined by observing the surfaces under a scanning electron microscope. As a result, the cyclic fatigue load of S and H1.0 were similar, and hollow specimens with outer diameters greater than 0.75 mm displayed the ability to withstand molar occlusal forces.Nonhuman primates are commonly used as experimental animals due to their biological resemblance to humans. In patients with cardiac disease, the levels of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) tend to increase in response to cardiac damage, and they are thus used as indicators for the diagnosis of human heart failure. However, no reference values for ANP and BNP have been reported for heart disease in nonhuman primates. In this study, we recorded the age, sex, and body weight of 202 cynomolgus monkeys, and performed evaluations to assess the ANP and BNP levels, electrocardiography and echocardiography, and accordingly divided the monkeys into two groups healthy monkeys and those with spontaneous cardiac disease. Statistical analysis was performed to determine the relationship of ANP and BNP with the factors of age, sex, and body weight. No significant relationship was found between the levels of ANP and BNP and the factors of age, sex, and body weight. However, both the ANP and BNP levels were significantly different between the healthy monkeys and monkeys with valvular disease. Similar to humans, the ANP and BNP levels tended to increase with the progression of cardiac disease in monkeys. Based on these results, we concluded that ANP and BNP are indicators of cardiac disease in nonhuman primates, and that this nonhuman primate cardiac disease model is applicable for cardiology research in humans.Texture analysis, as well as its broader category radiomics, describes a variety of techniques for image analysis that quantify the variation in surface intensity or patterns, including some that are imperceptible to the human visual system. Cerebral gliomas have been most rigorously studied in brain tumors using MR-based texture analysis (MRTA) to determine the correlation of various clinical measures with MRTA features. Promising results in cerebral gliomas have been shown in the previous MRTA studies in terms of the correlation with the World Health Organization grades, risk stratification in gliomas, and the differentiation of gliomas from other brain tumors. Multiple MRTA studies in gliomas have repeatedly shown high performance of entropy, a measure of the randomness in image intensity values, of either histogram- or gray-level co-occurrence matrix parameters. Similarly, researchers have applied MRTA to other brain tumors, including meningiomas and pediatric posterior fossa tumors.However, the value of MRTA in the clinical use remains undetermined, probably because previous studies have shown only limited reproducibility of the result in the real world. The low-to-modest generalizability may be attributed to variations in MRTA methods, sampling bias that originates from single-institution studies, and overfitting problems to a limited number of samples.To enhance the reliability and reproducibility of MRTA studies, researchers have realized the importance of standardizing methods in the field of radiomics. Another advancement is the recent development of a comprehensive assessment system to ensure the quality of a radiomics study. These two-way approaches will secure the validity of upcoming MRTA studies. The clinical use of texture analysis in brain MRI will be accelerated by these continuous efforts.