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lain the variance of microbial community 0.26, 0.21, 0.20, 0.15, and 0.14, respectively. DOC, nitrate nitrogen, and heavy metal ions such as Cu2+, Zn2+, Mn2+, and As3+ were also related to the proportion of some microorganisms in the water.Antibiotics discharged into the environment cause increased environmental resistance. Four types of antibiotics (quinolones, tetracyclines, macrolides, and β-lactams) were selected for this study. In a comparison with the municipal wastewater plant, the concentration and removal of antibiotics in influent and effluent of domestic wastewater treatment facilities of different scales in villages and towns was investigated using high-performance liquid chromatography (HPLC) and tandem mass spectrometry (MS). The results showed that the highest amount of ofloxacin in rural wastewater treatment facilities reached 32663.5 ng·L-1. Due to the different situations of influent fluctuation, discharge requirements, and management between urban and rural wastewater plants, only 33% of the rural domestic wastewater facilities detected an antibiotics removal rate of more than 60%. The effective removal of some antibiotics can be achieved when the rural domestic wastewater treatment facilities maintain the standard discharge of conventional pollutants.Microplastics (MPs, particle size less then 5 mm), as a new pollutant, have attracted wide attention in recent years. The distributions of MPs in effluent of a sewage treatment plant (STP) were examined. Surface water, sediment, and freshwater organism samples were taken from the STP discharge outlet in the Lijiang River tributary (S1), the confluence of tributaries and main streams in the Lijiang River (S2), and downstream locations in the Lijiang River (S3). The impact of STP discharge effluent on the characteristics and spatial distribution of MPs pollution in freshwater organisms was studied. The results showed that the freshwater organisms had a probability of uptake of MPs by 94.2%. The mean abundance of MPs in S1 (2.7 n·ind-1) was significantly higher than that of S3 (1.9 n·ind-1, P less then 0.05). The MPs found in S1 and S3 were mainly less then 0.10 mm, accounting for 46.0% and 30.5%, respectively. The fiber type of MPs was observed in the body of freshwater organisms. Polyethylene terephthalate was the major polymer form in S1 organisms, while polypropylene was the major polymer form in S3. The effluent discharged from the STP led to the accumulation of MPs in freshwater organisms.In order to study the pollution source and ecological risk assessment of polycyclic aromatic hydrocarbons (PAHs) in the Jiaxing river network, PAHs were analyzed by GC-MS. The results show that with the influence of rainfall and surface runoff in the wet season, the types and mass concentrations of PAHs in the river network of Jiaxing differ from the dry season. Ten and 16 priority PAHs were detected in the dry season and wet season, respectively. The average mass concentration of PAHs in the dry season was 143.83ng·L-1 and 73.47ng·L-1 in the wet season. The range of mass concentration of PAHs in the dry and wet season was 77.32-283.76ng·L-1 and 13.05-133.02ng·L-1, respectively, and 2-ring and 3-ring PAHs accounted for 79.18% in the dry season and 73.60% in the wet season. PAHs pollution in the river network of Jiaxing was at a low level compared with the domestic and foreign areas. The isomer ratio and principal component analysis found that the PAHs in the Jiaxing river network mainly come from urban non-point source pollution, combustion source, and traffic pollution in the dry season and wet season. The results of the Kalf risk entropy method show that in the dry season, naphthalene(Nap), acenaphthylene(Acy), acenaphthene(Ace), fluorene(Flu), phenanthrene(Phe), anthracene(Ant), fluoranthene(Fla), pyrene(Pyr), and benzo(a)anthracene(BaA) are at moderate ecological risk. In the wet season, Nap, Acy, Flu, Phe, Fla, Pyr, BaA, benzo(b)fluoranthene(BbF), benzo(k)fluoranthene(BkF), benzo(a)pyrene(BaP), indeno(1,2,3-cd)pyrene(InP), and benzo(g,h,i)perylene(BghiP) are at moderate ecological risk. In the dry season, ∑PAHs are at moderate ecological risk, and low in the wet season. On the whole, PAHs pollution in the Jiaxing river network presents moderate ecological risk levels, and measures to reduce the ecological risk of PAHs in the river network should be taken by the Departments concerned.Endocrine disrupting compounds (EDCs), pharmaceuticals, and personal care products (PPCPs) have been of great concern as emerging contaminants of aquatic environments. In this study, the risks of EDCs and PPCPs in the Yangtze River Basin were ranked, based on their environmental exposure datasets and ecotoxicity datasets. The prioritized chemicals were then identified. The results found that EDCs and personal care products were deemed to represent higher risks to aquatic organisms, for example, estrone, estriol, 17β-estradiol, bisphenol S, atrazine, triclocarban, and triclosan, while the risks of pharmaceuticals were 500-fold lower. Among the pharmaceuticals, antibiotics posed relatively higher risks. The Xiangjiang River and Honghu Lake with its surrounding rivers were hotspots where more chemicals were found with the highest concentrations, followed by Taihu Lake, Dongting Lake and the Yangtze River Delta. When comparing with algae and worms, fish were the most sensitive taxa to 17β-estradiol and 17α-ethynylestradiol. The results of this work provide sound guidance for the future monitoring and management of chemicals in China.To understand the quantitative effect of heavy rain on nitrogen and phosphorus pollution in river-net plain, daily observations of nutrient concentrations in two rivers, flowing into Lake Taihu, were conducted from 1st September, 2017 to 31st August, 2019. The daily rainfall was recorded by auto-recording meteorological stations located on the two rivers and the Taihu Laboratory for Lake Ecosystem Research. selleck compound Intensive sampling in different sections of the two rivers during Super Typhoon Lekima was also conducted in August 2019. Using these datasets, the influence of heavy rainfall on various forms of nitrogen and phosphorus concentrations in the rivers, and its environmental effects, were analyzed. The results showed that 16 heavy rainfall events (19 d) were observed in two years, 50% of which occurred in the summer season. In addition, heavy rainfall accounted for as much as 41.33% of the total rainfall over the entire year. After the period of heavy rainfall, the concentrations of various forms of nitrogen and phosphorus increased, and the particulate P generally exhibited the fastest response, usually peaking on the day of heavy rainfall.

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