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This study investigated the effect of low voltage electrostatic field (LVEF) on the microstructure damage and protein structure changes of prepared beef steak during freezing. The scanning electron microscopy results showed that LVEF-assisted freezing (LVEFF) minimized the gaps in the cross section between muscle fibers induced by freezing and thus improved fiber compactness. Furthermore, LVEFF reduced the length of the enlarged sarcomere, repaired the Z-line fractures, and intensified the dismission of the A band in the air-blast freezing (AF) process. The decreased carbonyl content and increased total sulfhydryl content indicated that LVEFF reduced protein oxidation in the freezing process. In addition, the results of Raman spectroscopy and fluorescence spectroscopy revealed that LVEFF minimized the changes in protein secondary and tertiary structures during freezing. In conclusion, utilization of LVEF in the freezing of prepared beef steak could reduce both the microstructure damage and protein structure changes in the freezing process.Exposure to early life trauma is common and confers risk for psychological disorders in adolescence, including posttraumatic stress disorder (PTSD). Trauma exposure and PTSD are also consistently linked to alterations in gray matter volume (GMV). Despite the quantity of structural neuroimaging research in trauma-exposed populations, little consensus exists amongst research groups on best practices for image processing method and manual editing procedures. The purpose of this report is to evaluate the utility of manual editing of magnetic resonance (MR) images for detecting PTSD-related group differences in GMV. Here, T1-weighted MR images from adolescent girls aged 11-17 were obtained and analyzed. selleck chemicals llc Two datasets were created from the FreeSurfer reconall pipeline, one of which was manually edited by trained research assistants. Gray matter regions of interest were selected and total volume estimates were entered into linear mixed effects models with method (manual edits or automated) as a within-subjects factor and group dummy-coded with PTSD as the reference group. Consistent with prior literature, individuals with PTSD demonstrated reduced GMV of the amygdala compared to trauma-exposed and non-trauma exposed controls, independent of editing method. Our results demonstrate that amygdala GMV reductions in PTSD are robust to certain methodological choices and do not suggest a benefit to the time-intensive manual editing pipeline in FreeSurfer for quantifying PTSD-related GMV.Postmortem computed tomography is now being used more commonly for routine forensic investigation. The use of 3D reconstruction techniques including virtual gastroscopy is effective and also improves the speed of interpretation, recognition, and description of specific clinical conditions. However, it has been unclear whether postmortem virtual endoscopy could be applicable for medicolegal autopsy or whether it could complement pathological examination at autopsy. Here, we investigated the applicability of postmortem virtual gastroscopy by reviewing 295 medicolegal autopsy cases seen at our institution, and found four cases in which the technique had been able to demonstrate features corresponding to changes that were evident at autopsy. Thus,postmortem virtual gastroscopy would have only rarely been effective forvisualizing any change in the stomach in such cases. In addition, we describe in detail three of those cases in which virtual gastroscopy had been able to visualize changes in the stomach, including a gastric ulcer, a polyp, and the presence of foamy fluid, which were all verified at autopsy. In those cases, virtual gastroscopy was useful for understanding features in the stomach of the deceased, which were revealed by axial images of the abdomen, to forensic pathologists who were not familiar with PMCT 2D images. Taken together, our findings suggest that postmortem virtual gastroscopy might help facilitate clear, straightforward sharing of information about PMCT images of complex anatomical structures among radiologists and forensic pathologists, as well as non-medical professionals with a limited knowledge of anatomy and physiology.Efficient halotolerant phosphorus accumulation microorganisms are of great significance for the treatment of high-salt wastewater. In this study, a halotolerant fungus strain named MSP8 was isolated and identified as Aureobasidium sp. Salinity resistance results showed that strain MSP8 can resist the salinity from 0% to 17%, and 77.2% phosphorus removal was achieved at the optimal salinity of 5%. The strain also showed wide environmental adaptability (pH of 3-7; temperature of 20-30 °C). Batch tests and scanning electron microscope equipped with an energy dispersive spectrometer (SEM-EDS) characterization results verified the key role of extracellular polymeric substance (EPS) secreted by MSP8 in phosphorus removal. The actual brewery and chemical wastewater treatments exhibited that above 53.5% of phosphorus can be removed by MSP8. The excellent adaptation of MSP8 made it a potential candidate for phosphorus removal especially in saline wastewater treatment.In current biological nitrogen removal (BNR) processes, most of ammonium in municipal wastewater is biologically transformed to nitrogen gas, making ammonium recovery impossible. Thus, this article aims to provide a holistic review with in-depth discussions on (i) current BNR processes for municipal wastewater treatment, (ii) environmental and economic costs behind ammonium in municipal wastewater, (iii) state of the art of ammonium recovery from municipal wastewater including anaerobic membrane bioreactor turning municipal wastewater to a liquid fertilizer, capturing ammonium in phototrophic biomass, waste activated sludge for land application, bioelectrochemical systems, biological conversion of ammonium to nitrous oxide as a fuel oxidizer, and adsorption, (iv) feasibility and challenge of adsorption for ammonium recovery from municipal wastewater and (v) innovative municipal wastewater reclamation processes coupled with ammonium recovery. Moving forward, municipal wastewater reclamation and resource recovery should be addressed under the framework of circular economy.

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