Barbourhutchison8662

24).

MSI prevalence in OGC was 12.4%, associated with antral localization and advanced age. Patients with MSI tumors had a lower cumulative incidence of recurrence after surgery. MSI phenotype was mainly associated with loss of MLH1 protein expression, MLH1 promotor hypermethylation and had no BRAFV600E mutation.

MSI prevalence in OGC was 12.4%, associated with antral localization and advanced age. Patients with MSI tumors had a lower cumulative incidence of recurrence after surgery. MSI phenotype was mainly associated with loss of MLH1 protein expression, MLH1 promotor hypermethylation and had no BRAFV600E mutation.Human angiotensin converting enzyme 2 (hACE2) mediates the cell entry of both SARS-CoV and SARS-CoV2 and can be used as a drug target. The DNA encoding the truncated hACE2 (30-356aa) was cloned into pET-28a (+) and expressed in Escherichia coli Rosetta (DE3). The recombinant hACE2 (rhACE2) was purified by affinity chromatography on a Ni-NTA column and characterized with SDS-PAGE and Western blot. The binding activity of rhACE2 to Spike protein of SARS-CoV2 was evaluated in S protein-overexpressed HEK293A cells (HEK293A-SP cells) through flow cytometry. The prokaryotic expression system is able to produce approximately 75 mg protein per liter, which would be useful for infection mechanism study, and drug screening and development of SARS-CoV2.

SARS-CoV-2 predisposes patients to secondary infections; however, a better understanding of the impact of coinfections on the outcome of hospitalized COVID-19 patients is still necessary.

To analyse death risk due to coinfections in COVID-19 patients.

The odds of death of 212 severely ill COVID-19 patients were evaluated, with detailed focus on the risks for each pathogen, site of infection, comorbidities and length of hospitalization.

The mortality rate was 50.47%. Fungal and/or bacterial isolation occurred in 89 patients, of whom 83.14% died. Coinfected patients stayed hospitalized longer and had an increased odds of dying (odds ratio (OR) 13.45; R

= 0.31). The risk of death was increased by bacterial (OR 11.28) and fungal (OR 5.97) coinfections, with increased levels of creatinine, leucocytes, urea and C-reactive protein. Coinfections increased the risk of death if patients suffered from cardiovascular disease (OR 11.53), diabetes (OR 6.00) or obesity (OR 5.60) in comparison with patients with these comorbidities but without pathogen isolation. The increased risk of death was detected for coagulase-negative Staphylococcus (OR 25.39), Candida non-albicans (OR 11.12), S.aureus (OR 10.72), Acinetobacter spp. (OR 6.88), Pseudomonas spp. (OR 4.77), and C.albicans (OR 3.97). The high-risk sites of infection were blood, tracheal aspirate, and urine. Patients with coinfection undergoing invasive mechanical ventilation were 3.8 times more likely to die than those without positive cultures.

Severe COVID-19 patients with secondary coinfections required longer hospitalization and had higher risk of death. The early diagnosis of coinfections is essential to identify high-risk patients and to determine the right interventions to reduce mortality.

Severe COVID-19 patients with secondary coinfections required longer hospitalization and had higher risk of death. The early diagnosis of coinfections is essential to identify high-risk patients and to determine the right interventions to reduce mortality.

Heliconia rostrata Ruiz and Pav. belongs to the family Heliconiaceae. Plant was traditionally used to cure jaundice, intestinal pain, diabetes and hypertension.

Present study evaluated hepatoprotective efficacy of ethanol (REE) and methanol (RME) extracts of H. check details rostrata rhizomes in HepG2 cell lines and rats. Antioxidant efficacy of extracts was determined using ex vivo and in vivo methods.

Before conducting efficacy studies, safety of REE and RME was established using toxicity studies which included Oral acute-fixed dose toxicity using OECD TG420, 28-days repeated dose oral toxicity by OECD TG407 and cytotoxicity studies by brine shrimp lethality (BSL) bioassay and MTT assay taking HepG2 cell line. Ex vivo (Extracts 0-250μg/ml) and in vivo (Extracts 50, 100 and 200mg/kg) antioxidant studies were performed on fresh goat liver and rats (N=45) of either sex, respectively. In vitro hepatoprotective efficacy of extracts was evaluated against ethanol induced toxicity in HepG2 cell line. In vivo study was perfced hepatotoxicity. At 400μg/ml, REE and RME demonstrated percentage protection of 65.53% and 57.98%, respectively. Results of liver function test and histopathological evaluation of liver in in vivo hepatoprotective study confirmed dose dependent protection provided by the extracts against CCl

-induced hepatotoxicity.

Both REE and RME were found safe to be considered for therapeutic uses. Both REE and RME were found to exhibit antioxidant efficacy in ex vivo and in vivo models. Results ascertained that H. rostrata rhizomes possess significant hepatoprotective potency.

Both REE and RME were found safe to be considered for therapeutic uses. Both REE and RME were found to exhibit antioxidant efficacy in ex vivo and in vivo models. Results ascertained that H. rostrata rhizomes possess significant hepatoprotective potency.

Elaeagnus conferta Roxb. (Elaeagnaceae) is a subtropical shrub mainly native to India, Vietnam, Malaysia and South China, whose various parts are used for treatment of diabetes, gastric ulcers, pain, oxidative stress and pulmonary disorders. Though the other parts of the plant have been reported for their ethnic use i.e. fruits as astringent locally and for cancer systemically, leaves for body pain and flowers for pain in chest and the seeds are mentioned as edible, there is no report per se on the medicinal use of seeds. Based on the fact that seeds of closely resembling species i.e. Elaeagnus rhamnoides has demonstrated significant anti-gastroulcerative property, the probability of the seeds of E. conferta possessing similar activity seemed quite significant.

Phytochemical investigation and assessment of pharmacological mechanism(s) involved in anti-ulcer effect of methanolic extract of the seeds of E. conferta.

Bioactive phytoconstituents were isolated by column chromatography. These were identified by spectroscopic techniques including infrared (IR) spectroscopy, nuclear magnetic resonance (NMR) and mass spectrometry.