Rushdwyer2451

Technological advances in image-based platelet analysis or platelet morphometry are critical for a better understanding of the structure and function of platelets in biological research as well as for the development of better clinical strategies in medical practice. Recently, the advent of high-throughput optical imaging and deep learning has boosted platelet morphometry to the next level by providing a new set of capabilities beyond what is achievable with traditional platelet morphometry, shedding light on the unexplored domain of platelet analysis. This Opinion article introduces emerging opportunities in 'intelligent' platelet morphometry, which are expected to pave the way for a new class of diagnostics, pharmacometrics, and therapeutics.

There is growing concern regarding the impact of general anesthesia on neurodevelopment in children. Pre-clinical animal studies have linked anesthetic exposure to abnormal central nervous system development, but it is unclear whether these results translate into humans. The purpose of this systematic review from the American Pediatric Surgical Association (APSA) Outcomes and Evidence-Based Practice (OEBP) Committee was to review, summarize, and evaluate the evidence regarding the neurodevelopmental impact of general anesthesia on children and identify factors that may affect the risk of neurotoxicity.

Medline, Cochrane, Embase, Web of Science, and Scopus databases were queried for articles published up to and including December 2017 using the search terms "general anesthesia and neurodevelopment" as well as specific anesthetic agents. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were used to screen manuscripts for inclusion in the review. A consensus statemenrform general anesthesia for single short procedures. With the complexities and nuances of different anesthetic methods, patients and procedures, the planning and execution of anesthesia for the pediatric patient is generally best accomplished by an anesthesiologist, ideally a pediatric anesthesiologist.

Systematic review of level 1-4 studies.

Level 1-4 (mainly level 3-4).

Level 1-4 (mainly level 3-4).

Conventional median sternotomy (CMS) is still the standard technique utilized to implant left ventricular assist devices (LVADs). Recent studies suggest that less invasive surgery (LIS) may be beneficial; however, robust data on differences in right heart failure (RHF) are lacking. This study aimed to determine the impact of LIS compared with that of CMS on RHF outcomes after LVAD implantation.

An international multicenter retrospective cohort study was conducted across 5 centers. Patients were grouped according to their implantation technique (LIS vs CMS). Only centrifugal devices were included. RHF was defined as severe or severe acute RHF according to the 2013 Interagency Registry for Mechanically Assisted Circulatory Support (INTERMACS) definition. Logistic multivariate regression and propensity score‒matched analyses were performed to account for confounding.

Overall, 427 implantations occurred during the study period, with 305 patients implanted using CMS and 122 using LIS. Pre-operative extracorphere was no difference in 1-year survival. LIS is an alternative to traditional CMS.

LIS may be associated with less RHF after LVAD implantation compared with CMS. Despite the possible reduction in RHF, there was no difference in 1-year survival. LIS is an alternative to traditional CMS.

To assess the capillary vessel density in the peripapillary region of glaucoma patients in comparison to controls using automated software.

We performed an observational, cross-sectional case-control study with 72 eyes of glaucoma patients (with strict selection criteria) and 30 age-matched healthy controls. We used an optical coherence tomography angiography device (Triton, Topcon) with scans of 4.5×4.5mm centered on the optic nerve head, excluding images of poor quality. Since this device does not quantify vessel density, we wrote software (with Visual Studio©) that allowed us to remove large blood vessels and quantify the capillary density in the peripapillary region. We tested the ability of OCT-A to differentiate glaucoma from controls using the receiver operating characteristic curve (ROC). We also verified whether VD was correlated with visual field mean deviation.

VD was significantly lower in glaucoma than in controls, notably the whole-image VD of 72.45±7.45% in glaucoma and 77.87±3.77% in controls (P<0.001). VD values were correlated with visual fields (Spearman correlation of 0.33; P 0.0017). ROC was moderate (color VD of 0.629±0.143), but better than that of the visual field (0.229±0.077) or the retinal nerve fiber layer thickness assessed by OCT (0.326±0.064).

This software is useful for quantifying vessel density at the optic disc, peripapillary and capillary levels and for documenting glaucomatous changes in vascularization. OCT-A might be useful in diagnosis of glaucoma.

This software is useful for quantifying vessel density at the optic disc, peripapillary and capillary levels and for documenting glaucomatous changes in vascularization. OCT-A might be useful in diagnosis of glaucoma.Lysosomal degradation of endoplasmic reticulum (ER) fragments by autophagy, termed ER-phagy or reticulophagy, occurs under normal as well as stress conditions. The recent discovery of multiple ER-phagy receptors has stimulated studies on the roles of ER-phagy. We discuss how the ER-phagy receptors and the cellular components that work with these receptors mediate two important functions ER homeostasis and ER quality control. We highlight that ER-phagy plays an important role in alleviating ER expansion induced by ER stress, and acts as an alternative disposal pathway for misfolded proteins. We suggest that the latter function explains the emerging connection between ER-phagy and disease. Additional ER-phagy-associated functions and important unanswered questions are also discussed.The treatment of myotonic dystrophy type 1 (DM1) focuses on reducing symptom burden. Hydroxychloroquine However, since medication often fails to produce satisfying symptom relief, some patients seek alternatives, such as cannabis, to help reduce some of these symptoms. The aim of this study was to provide an accurate profile of cannabis use among DM1 patients. Phone interviews were conducted to identify current and former users, and to assess reasons for cannabis use. Characteristics of cannabis use were also investigated. Briefly, among the 72 study participants, 22.2% currently used cannabis and a majority of them (56.9%) reported using it to relieve symptoms associated with DM1. These users, classified as therapeutic users, reported poorer health status (EQ-5D index scores 0.532±0.230 vs. 0.823±0.208, p = 0.020; EQ-VAS scale 50.56±10.74 vs. 75.57±21.50, p = 0.009) than non-therapeutic users. Finally, differences among sex were also highlighted. While the therapeutic effects of cannabis were not explored in our study, our results support the potential role of cannabis and cannabinoids in the treatment of DM1-associated symptoms which will need to be thoroughly investigated.