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Norbormide [5-(α-hydroxy-α-2-pyridylbenzyl)-7-(α-2-pyridylbenzylidene)-5-norbornene-2,3-dicarboximide] (NRB, 1), an existing but infrequently used rodenticide, is known to be uniquely toxic to rats, but relatively harmless to other rodents and mammals. As a vasoactive agent, NRB induces a species-specific vasocontractile effect that is restricted to the peripheral arteries of the rat. Despite the precise mechanisms behind this phenomenon having yet to be fully clarified, it is postulated that the molecular target of NRB could be located within the plasma membrane of rat peripheral artery myocytes (e.g. rat caudal artery myocytes). As such, the primary objective of this study was to develop a fluorescently labelled derivative of NRB to investigate its subcellular distribution/localization in both NRB-sensitive (freshly isolated rat caudal artery myocytes, FIRCAMs) and NRB-insensitive (human hepatic stellate, LX2) cells. Of the examples prepared, lead structure endo-NRB-NBD-bPA subsequently demonstrated retention of the parent toxicant's pharmacological profile (in terms of its ability to induce both a vasocontractile response in rat caudal artery rings in vitro, and a lethal end-point in rats in vivo). Endo-NRB-NBD-bPA was also shown to be significantly less permeable (an integral feature in the design of fluorescent probes targeting cell-surface receptors) to both LX2 cells and FIRCAMs. Disappointingly, no fluorescence could be observed on the plasma membrane of FIRCAMs stained with endo-NRB-NBD-bPA.Proteus mirabilis is one of the Enterobacteriales species that has been deemed as non-mutable by DNA damaging agents. A genomic analysis of P. mirabilis genomes shows that this species often does not carry pol V-encoding genes in its chromosome, which are responsible for SOS mutagenesis in other bacteria. On the other hand, the highly active umuDC homologs rumAB are carried in the mobile integrative conjugative elements (ICEs) from the SXT/R391 family that are frequently found in this species. Here we show that isolates devoid of SXT/R391 are indeed weakly or non-mutable by UV-light exposure, in contrast to isolates carrying SXT/R391 elements, some of which are mutable under these conditions. SXT/R391-bearing isolates display a variable behavior regarding UV-induced mutagenesis, despite the functionality of rumAB homologs carried by them. We also show that the globally dispersed ICEPmiJpn1 confers UV mutability to otherwise non-mutable isolates and demonstrate that this phenomenon is dependent on rumAB genes. Finally, we investigated whether rumAB genes could play a role in the conjugation of ICEPmiJpn1 and found that these genes improve the conjugation of SXT/R391 by a small margin. WZ4003 chemical structure Taken together, these results show that the presence of rumAB in SXT/R391 ICEs endows the hosts with damage-inducible mutagenesis ability and promotes a small but significant enhancement in element transfer after exposure to UV light.River ice in the upper Yarlung Zangbo River is characterized by seasonal freezing-thawing cycles (SFTC). It is important to explore the effects of SFTC on phosphorus release and transformation from upstream surface sediments to protect the ecosystem of the Yarlung Zangbo River. The process and mechanism of phosphorus release and transformation in sediments following SFTC were investigated in a laboratory simulation experiment. The results showed that after freezing, sediment particles were broken, the specific surface area was increased by 14%, and the particle size was decreased by 43%, which resulted in weakened adsorption of phosphorus by sediments. Moreover, the destruction of organic matter (OM) on the sediment surface will release more ion adsorption sites and promote the release of phosphorus. The bioavailabilities of exchangeable phosphorus (Ex-P), aluminum phosphorus (Al-P) and iron phosphorus (Fe-P) increased by 60.09%, 86.86% and 31.86%, respectively, after freezing. Organic phosphorus (O-P) is used indirectly by organisms, and O-P content showed a significant correlation with OM content. Water affected the oxygen content in sediments during the freezing period, and continuous hypoxia promoted the release and transformation of Fe-P and Al-P.Whether muscle properties gradually change with age and how muscle properties are affected by sex remain unclear. In this study, we investigated the influence of age and sex on the biomechanical and viscoelastic properties of arm muscles in middle-aged and older adults. In this cross-sectional study, 80 healthy participants were divided by sex (male and female), and each sex group, by 10-year age ranges (40-49, 50-59, 60-69, and 70-79 years). Muscle properties, including tone, stiffness, elasticity, and mechanical stress relaxation time, were measured with the MyotonPRO. Our results showed that the muscle tone and elasticity of the deltoid and flexor carpi radialis, and the muscle tone of the flexor carpi ulnaris, were significantly greater in men than in women, whereas the stress relaxation time of the triceps was significantly greater in women than in men. Significantly greater muscle stiffness in the biceps brachii was found in the participants over 50 years old. Less muscle elasticity was found in the deltoid, triceps, and flexor carpi ulnaris in those over 70 years old. In conclusion, age and sex have considerable impacts on upper-limb muscle properties in middle-aged and older adults, which should be taken into consideration when planning health promotion projects.

During the COVID-19 pandemic, universities adopted online teaching as the primary teaching and learning method. Most of the online teaching, however, has been limited to the broadcasting of asynchronous lectures and sharing of teaching materials.

To explore undergraduate nursing students' self-regulated learning experiences, satisfaction, and attitudes toward older persons in a gerontological nursing course using online self-regulated enquiry-based learning (EBL) during the COVID-19 pandemic.

A mixed-methods design was adopted.

A university in Hong Kong providing pre-registration nursing programmes.

155 students studying in the fourth year in a five-year Bachelor of Nursing programme participated in the quantitative study in which 18 joined the focus groups (4 groups with group size of 4-5).

Five video-based scenarios along with two interactive online workshops, a mind-map development exercise, gamified assessments, reading materials, group discussions, and presentations were adopted in a gerontolroved the students' online learning experience in a gerontological nursing course during the pandemic and improved their attitudes toward older people.

The online self-regulated EBL improved the students' online learning experience in a gerontological nursing course during the pandemic and improved their attitudes toward older people.

The mechanisms that contribute to continued male intrauterine growth in response to an adverse maternal environment, such as those brought on by maternal asthma, remain largely undefined but may, in part, be mediated by androgen-mediated signalling. We previously reported the expression of multiple AR protein isoforms in the human placenta and proposed the novel AR-45 isoform to be integral in mediating male-specific androgen-dependent signalling in the presence of maternal asthma. In the current study we have used an ex vivo approach to further understand sex-specific differences in placental androgen signalling in the presence and absence of inflammation using human term villous placental explants.

Explants were cultured in the presence and absence of 0.1nM dihydrotestosterone (DHT), 1μg/ml lipopolysaccharide (LPS), or DHT+LPS for 24hr. Tissue was used for gene expression and subcellular AR protein isoform expression.

Cytoplasmic and nuclear AR protein isoforms expression did not vary between culture to further characterise the function of this AR isoform.The aim of this study was to examine the impact of backpack load carriage on lower limb strength and loading rate change in a cohort that match military recruit profiles. Twenty-six participants walked on a treadmill either carrying a military load carriage system (32 kg) or unloaded for 2 h on two consecutive days. Participants ground reaction forces and strength measures were assessed using a force platform and dynamometry, respectively. Testing included assessments before and after treadmill walking on days one and two, and 24 h following day 2. When assessed by mixed methods ANOVA (alpha 0.05) statistically significant interaction effects were observed for loading peak (p = 0.031), loading rate (p = 0.035) and plantarflexor torque dynamometry variables at 60°s-1 (p = 0.011) and 120°s-1 (p = 0.024). Repeated measures correlation highlighted associations between plantarflexor torque at 60°s-1 and loading rate (r = -0.901, p less then 0.001). Load carriage reduced lower limb torque which did not recover between days. Plantarflexor torque reductions were associated with increases in loading rate. Practitioners should consider that load bearers are more likely to experience lower limb injury during multi-day load carriage. Future work should develop protocols to reduce plantarflexor torque loss in order to reduce ground reaction force change.We compared the effects of using a commercial exoskeleton on shoulder muscle activity, task completion time, perceived effort and comfort while performing four tasks in different shoulder positions. Fourteen automotive industry workers performed four simulated tasks with shoulder at A≈0°, B ≈ 45°, C ≈ 90° and D ≈ 115° flexion. The electromyographic activity of the Medial Deltoid (MD) and the Anterior Deltoid (AD) decreased when wearing the exoskeleton. The effect sizes (ES) were, for MD ES = 0, ES = -0.2, ES = -0.6, ES = -0.3; and for AD ES = 0.3, ES = -0.6, ES = -0.8, ES = -0.6; for tasks A, B, C and D, respectively. We also found increased Anterior Deltoid/Triceps Brachii co-contraction, a typical joint stabilization mechanism. Wearing the exoskeleton increased the completion time of task B and reduced the perceived effort of tasks A and C, improving overall comfort. These findings are useful to organize the logistics of the workstations that use upper limb exoskeletons to improve the effectiveness of this equipment.

Glypican-4 (GPC4) is a cell surface protein, but can be released into circulation under various clinical conditions. The association of circulating GPC4 with the risk of future cardiovascular events or death is unclear. In the present study, we aimed to investigate the association between serum GPC4 and major adverse cardiovascular events (MACE), vascular mortality, and all-cause mortality in a prospective cohort study.

Our study included 760 patients undergoing coronary angiography. During a mean follow up period of 6.3 years, the incidence of MACE, vascular mortality, and all-cause mortality was recorded. Serum GPC4 levels were determined using an enzyme-linked immunosorbent assay.

Serum GPC4 was highly significantly associated with increased age, body mass index, brain natriuretic peptide, and oxidized low density lipoprotein, as well as with decreased estimated glomerular filtration rate. During the follow-up period, 145 patients died, including 67 vascular deaths. MACE occurred in 137 patients. Serum GPC4 was significantly associated with MACE, vascular mortality, and all-cause mortality independently of traditional cardiovascular risk factors, with adjusted hazard ratios (HR) and 95% confidence intervals for one standard deviation change of serum GPC4 of 1.