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A central challenge in protein modeling research and protein structure prediction in particular is known as decoy selection. The problem refers to selecting biologically-active/native tertiary structures among a multitude of physically-realistic structures generated by template-free protein structure prediction methods. Research on decoy selection is active. Clustering-based methods are popular, but they fail to identify good/near-native decoys on datasets where near-native decoys are severely under-sampled by a protein structure prediction method. Reasonable progress is reported by methods that additionally take into account the internal energy of a structure and employ it to identify basins in the energy landscape organizing the multitude of decoys. These methods, however, incur significant time costs for extracting basins from the landscape. In this paper, we propose a novel decoy selection method based on non-negative matrix factorization. We demonstrate that our method outperforms energy landscape-based methods. In particular, the proposed method addresses both the time cost issue and the challenge of identifying good decoys in a sparse dataset, successfully recognizing near-native decoys for both easy and hard protein targets.This paper describes a semi-powered ankle prosthesis and corresponding unified controller that provides biomimetic behavior for level and sloped walking without requiring identification of ground slope or modulation of control parameters. The controller is based on the observation that healthy individuals maintain an invariant external quasi-stiffness (spring like behavior between the shank and ground) when walking on level and sloped terrain. Emulating an invariant external quasi-stiffness requires an ankle that can vary the set-point (i.e., equilibrium angle) of the ankle stiffness. A semi-powered ankle prosthesis that incorporates a novel constant-volume power-asymmetric actuator was developed to provide this behavior, and the unified controller was implemented on it. The device and unified controller were assessed on three subjects with transtibial amputations while walking on inclines, level ground, and declines. Experimental results suggest that the prosthesis and accompanying controller can provide a consistent external quasi-stiffness similar to healthy subjects across all tested ground slopes.Paralysis of the upper extremity severely restricts independence and quality of life after spinal cord injury. Regaining control of hand and arm movements is the highest treatment priority for people with paralysis, 6-fold higher than restoring walking ability. Nevertheless, current approaches to improve upper extremity function typically do not restore independence. Spinal cord stimulation is an emerging neuromodulation strategy to restore motor function. selleck chemical Recent studies using surgically implanted electrodes demonstrate impressive improvements in voluntary control of standing and stepping. Here we show that transcutaneous electrical stimulation of the spinal cord leads to rapid and sustained recovery of hand and arm function, even after complete paralysis. Notably, the magnitude of these improvements matched or exceeded previously reported results from surgically implanted stimulation. Additionally, muscle spasticity was reduced and autonomic functions including heart rate, thermoregulation, and bladder function improved. Perhaps most striking is that all six participants maintained their gains for at least three to six months beyond stimulation, indicating functional recovery mediated by long-term neuroplasticity. Several participants resumed their hobbies that require fine motor control, such as playing the guitar and oil painting, for the first time in up to 12 years since their injuries. Our findings demonstrate that non-invasive transcutaneous electrical stimulation of the spinal networks restores movement and function of the hands and arm for people with both complete paralysis and long-term spinal cord injury.We propose a novel framework to produce cartoon videos by fetching the color information from two input keyframes while following the animated motion guided by a user sketch. The key idea of the proposed approach is to estimate the dense cross-domain correspondence between the sketch and cartoon video frames, and employ a blending module with occlusion estimation to synthesize the middle frame guided by the sketch. After that, the input frames and the synthetic frame equipped with established correspondence are fed into an arbitrary-time frame interpolation pipeline to generate and refine additional inbetween frames. Finally, a module to preserve temporal consistency is employed. Compared to common frame interpolation methods, our approach can address frames with relatively large motion and also has the flexibility to enable users to control the generated video sequences by editing the sketch guidance. By explicitly considering the correspondence between frames and the sketch, we can achieve higher quality results than other image synthesis methods. Our results show that our system generalizes well to different movie frames, achieving better results than existing solutions.The recent advance in motion tracking (e.g., Visual Inertial Odometry) allows the use of a mobile phone as a 3D pen, thus significantly benefiting various mobile Augmented Reality (AR) applications based on 3D curve creation. However, when creating 3D curves on and around physical objects with mobile AR, tracking might be less robust or even lost due to camera occlusion or textureless scenes. This motivates us to study how to achieve natural interaction with minimum tracking errors during close interaction between a mobile phone and physical objects. To this end, we contribute an elicitation study on input point and phone grip, and a quantitative study on tracking errors. Based on the results, we present a system for direct 3D drawing with an AR-enabled mobile phone as a 3D pen, and interactive correction of 3D curves with tracking errors in mobile AR. We demonstrate the usefulness and effectiveness of our system for two applications in-situ 3D drawing, and direct 3D measurement.Diffuse reverberation is ultrasound image noise caused by multiple reflections of the transmitted pulse before returning to the transducer, which degrades image quality and impedes the estimation of displacement or flow in techniques such as elastography and Doppler imaging. Diffuse reverberation appears as spatially incoherent noise in the channel signals, where it also degrades the performance of adaptive beamforming methods, sound speed estimation, and methods that require measurements from channel signals. In this paper, we propose a custom 3D fully convolutional neural network (3DCNN) to reduce diffuse reverberation noise in the channel signals. The 3DCNN was trained with channel signals from simulations of random targets that include models of reverberation and thermal noise. It was then evaluated both on phantom and in-vivo experimental data. The 3DCNN showed improvements in image quality metrics such as generalized contrast to noise ratio (GCNR), lag one coherence (LOC) contrast-to-noise ratio (CNR) and contrast for anechoic regions in both phantom and in-vivo experiments. Visually, the contrast of anechoic regions was greatly improved. link2 The CNR was improved in some cases, however the 3DCNN appears to strongly remove uncorrelated and low amplitude signal. In images of in-vivo carotid artery and thyroid, the 3DCNN was compared to short-lag spatial coherence (SLSC) imaging and spatial prediction filtering (FXPF) and demonstrated improved contrast, GCNR, and LOC, while FXPF only improved contrast and SLSC only improved CNR.This paper addresses the task of detecting and recognizing human-object interactions (HOI) in images. Considering the intrinsic complexity and structural nature of the task, we introduce a cascaded parsing network (CP-HOI) for a multi-stage, structured HOI understanding. At each cascade stage, an instance detection module progressively refines HOI proposals and feeds them into a structured interaction reasoning module. Each of the two modules is also connected to its predecessor in the previous stage. The structured interaction reasoning module is built upon a graph parsing neural network (GPNN). In particular, GPNN infers a parse graph that i) interprets meaningful HOI structures by a learnable adjacency matrix, and ii) predicts action (edge) labels. Within an end-to-end, message-passing framework, GPNN blends learning and inference, iteratively parsing HOI structures and reasoning HOI representations (i.e., instance and relation features). Further beyond relation detection at a bounding-box level, we make our framework flexible to perform fine-grained pixel-wise relation segmentation; this provides a new glimpse into better relation modeling. link3 A preliminary version of our CP-HOI model reached 1st place in the ICCV2019 Person in Context Challenge, on both relation detection and segmentation. Our CP-HOI shows promising results on two popular HOI recognition benchmarks, i.e., V-COCO and HICO-DET.

Asthma and chronic obstructive pulmonary disease (COPD) can be confused in clinical diagnosis due to overlapping symptoms. The purpose of this study is to develop a method based on multivariate pulmonary sounds analysis for differential diagnosis of the two diseases.

The recorded 14-channel pulmonary sound data are mathematically modeled using multivariate (or, vector) autoregressive (VAR) model, and the model parameters are fed to the classifier. Separate classifiers are assumed for each of the six sub-phases of flow cycle, namely, early/mid/late inspiration and expiration, and the six decisions are combined to reach the final decision. Parameter classification is performed in the Bayesian framework with the assumption of Gaussian mixture model (GMM) for the likelihoods, and the six sub-phase decisions are combined by voting, where the weights are learned by a linear support vector machine (SVM) classifier. Fifty subjects are incorporated in the study, 30 being diagnosed with asthma and 20 with COPD.

The highest accuracy of the classifier is 98 percent, corresponding to correct classification rates of 100 and 95 percent for asthma and COPD, respectively. The prominent sub-phase to differentiate between the two diseases is found to be mid-inspiration.

The methodology proves to be promising in terms of asthma-COPD differentiation based on acoustic information. The results also reveal that the six sub-phases are not equally pertinent in the differentiation.

Pulmonary sounds analysis may be a complementary tool in clinical practice for differential diagnosis of asthma and COPD, especially in the absence of reliable spirometric testing.

Pulmonary sounds analysis may be a complementary tool in clinical practice for differential diagnosis of asthma and COPD, especially in the absence of reliable spirometric testing.High-frequency irreversible electroporation (H-FIRE) is a tissue ablation modality employing bursts of electrical pulses in a positive phaseinterphase delay (d1)negative phaseinterpulse delay (d2) pattern. Despite accumulating evidence suggesting the significance of these delays, their effects on therapeutic outcomes from clinically-relevant H-FIRE waveforms have not been studied extensively.

We sought to determine whether modifications to the delays within H-FIRE bursts could yield a more desirable clinical outcome in terms of ablation volume versus extent of tissue excitation.

We used a modified spatially extended nonlinear node (SENN) nerve fiber model to evaluate excitation thresholds for H-FIRE bursts with varying delays. We then calculated non-thermal tissue ablation, thermal damage, and excitation in a clinically relevant numerical model.

Excitation thresholds were maximized by shortening d1, and extension of d2 up to 1,000 's increased excitation thresholds by at least 60% versus symmetric bursts.