Workmantrue2753

DMPA-SC was the first method used by the majority of current users in Burkina Faso and Uganda. DMPA-SC users who previously used another method generally switched from less effective methods.

Although the characteristics of women using DMPA-SC varied across countries, DMPA-SC appears to be reaching new populations of women instead of inspiring existing modern users to switch to DMPA-SC, and appears to be appealing to first time users of contraception.

It appears that DMPA-SC appeals to new contraceptive users in sub-Saharan Africa, which implies that DMPA-SC may have the potential to increase modern contraceptive prevalence in sub-Saharan African countries.

It appears that DMPA-SC appeals to new contraceptive users in sub-Saharan Africa, which implies that DMPA-SC may have the potential to increase modern contraceptive prevalence in sub-Saharan African countries.Anaerobes are a major constituent of the gut microbiome and profoundly influence the overall health of humans. However, the lack of a simple, cost-effective, and scalable system that mimics the anaerobic conditions of the human gut is hindering research on the gut microbiome and the development of therapeutics. Here, we address this gap by using glucose oxidase and catalase containing gelatin microparticles (GOx-CAT-GMPs) to precisely regulate dissolved oxygen concentration [O2] via GOx-mediated consumption of oxygen. Fluorescence images generated using conjugated polymer afterglow nanoparticles showed that [O2] can be tuned from 257.9 ± 6.2 to 0.0 ± 4.0 μM using GOx-CAT-GMPs. Moreover, when the obligate anaerobe Bacteroides thetaiotaomicron was inoculated in media containing GOx-CAT-GMPs, bacterial growth under ambient oxygen was comparable to control conditions in an anaerobic chamber (5.4 × 105 and 8.8 × 105 colony forming units mL-1, respectively). Finally, incorporating GOx-CAT-GMPs into a bioreactor that permitted continuous radial diffusion of oxygen and glucose generated a gut-mimetic [O2] gradient of 132.4 ± 2.6 μM in the outer ring of the reactor to 7.9 ± 1.7 μM at the core. Collectively, these results indicate that GOx-CAT-GMPs are highly effective oxygen-regulating materials. These materials can potentially be leveraged to advance gut microbiome research and fecal microbiota transplantation, particularly in low-resource settings.We present a case of drug-resistant focal motor seizures in which separate cortico-cortical epileptic networks within the supplementary motor area (SMA) proper and primary motor area (PMA) were proven by ictal high-frequency oscillation (HFO) and cortico-cortical evoked potential (CCEP). A 12-year-old girl presented with two types seizures type A, tonic extension and subsequent clonic movements of the right arm; and type B, tonic and clonic movements of the right leg. MRI was normal and karyotype genetic analysis revealed 46,X,t(X;14)(q13;p12). She underwent placement of chronic subdural electrodes over the left hemisphere. We recorded a total of nine seizures during 10 days of epilepsy monitoring. Type A seizures started from the lower part of the left SMA proper and early spread to the hand motor area of the PMA. Type B seizures started from the upper part of the SMA proper and early spread to the leg motor area of the PMA. CCEPs of both SMA proper and PMA activated two identical routes for evoked potentials correlating with separate pathways. Corticectomy of the left SMA proper and PMA achieved seizure-free without hemiparesis. Within a small homunculus of the SMA proper, separate epileptic networks were proven and validated by seizure semiology, ictal HFO, and CCEP.SV2A encodes a neuronal synaptic vesicle glycoprotein essential for neurotransmitter release. Altered SV2A function leads to epilepsy in animal models, yet only two reports of human variants have linked SV2A to syndromic drug-resistant epileptic encephalopathies and epilepsy. SV2A is also the binding site for the commonly used antiseizure medication levetiracetam (LEV). However, information about how rare SV2A variants influence LEV response is lacking. Here, we report a two-year-old child with new-onset epilepsy found to have a de novo heterozygous rare variant in SV2A (NM_014849.5c.1978G>A;p.Gly660Arg) who developed refractory status epilepticus after escalation of LEV treatment for initial baseline seizure control. This report provides additional evidence that monoallelic pathogenic SV2A variants cause epilepsy and that genetic variation in SV2A could lead to paradoxical seizure worsening when treated with LEV.Epidemiologists have used trihalomethanes (THMs) as a surrogate for overall disinfection byproduct (DBP) exposure based on the assumption that THM concentrations are proportional to concentrations of other DBP classes. Selleck PP2 Toxicological evidence indicates THMs are less potent toxins than unregulated classes like haloacetonitriles (HANs). If THMs are not proportional to the DBPs driving toxicity, the use of THMs to measure exposure may introduce non-trivial exposure misclassification bias in epidemiologic studies. This study developed statistical models to evaluate the covariance and proportionality of HAN and THM concentrations in a dataset featuring over 9500 measurements from 248 public water systems. THMs only explain ∼30% of the variance in HANs, whether the data is pooled in a classic linear regression or hierarchically grouped by water system in a multilevel linear regression. The 95% prediction interval on HANs for the median THM concentration exceeds the interquartile range of HANs. Mean HANTHM ratios range from ∼2.4% to ∼80% across water systems, and varied with source water category, season, disinfectant sequence and distribution system location. The HANTHM ratio was 265% higher in groundwater systems than in surface water systems and declined by ∼40% between finished effluent and maximum residence times in surface water systems with chlorine-chlorine disinfection. A maximum likelihood approach was used to estimate the misclassification bias that may result from using THMs to construct risk-ratios, assuming that HANs represent the "true" DBP exposure risk. The results indicate an odds ratio of ∼2 estimated with THM concentrations could correspond to a true odds ratio of 4-5. These findings demonstrate the need for epidemiologic studies to evaluate exposure by measuring DBPs that are likely to drive toxicity.