Elliscrawford4319

Modifying the electrodes of microbial fuel cells (MFCs) with iron oxides can improve the bacterial attachment performances and electrocatalytic activities for energy conversion, which is of significance in the fabrication of MFCs. However, the conventional modification methods usually result in the aggregation of iron sites, producing the electrodes of poor qualities. Herein, we report a novel method for the modification of electrochemical electrodes to boost the anode performance of MFC. The Shewanella precursor adhered on carbon felt electrode was directly carbonized to form a bacteria-derived biological iron oxide/carbon (Bio-FeOx/C) nanocomposite catalyst. The large spatial separation between the bacteria, as well as those between the iron containing proteins in the bacteria, deliver a highly dispersed Bio-FeOx/C nanocomposite with good electrocatalytic activities. The excellent microbial attachment performance and electron transfer rate of the Bio-FeOx/C modified electrode significantly promote the transfer of produced electrons between bacteria and electrode. Accordingly, the MFC with the Bio-FeOx/C electrode exhibits the maximum power density of 797.0 mW m-2, much higher than that obtained with the conventional carbon felt anode (226.1 mW m-2). Our works have paved a new avenue to the conversion of the natural bacterial precursors into active iron oxide nanoparticles as the anode catalyst of MFCs. The high catalytic activity of the prepared Bio-FeOx endows it great application potentials in the construction of high-performance electrodes.Cyanobacterial blooms are observed when high cell densities occur and are often dangerous to human and animal health due to the presence of cyanotoxins. Conventional drinking water treatment technology struggles to efficiently remove cyanobacterial cells and their metabolites during blooms, increasing costs and decreasing water quality. Although field applications of hydrogen peroxide have been shown to successfully suppress cyanobacterial growth, a rapid and accurate measure of the effect of oxidative stress on cyanobacterial cells is required. In the current study, H2O2 (5 and 20 mg L-1) was used to induce oxidative stress in Microcystis aeruginosa PCC 7813. Cell density, quantum yield of photosystem II, minimal fluorescence and microcystin (MC-LR, -LY, -LW, -LF) concentrations were compared when evaluating M. aeruginosa cellular stress. Chlorophyll content (determined by minimal fluorescence) decreased by 10% after 48 h while cell density was reduced by 97% after 24 h in samples treated with 20 mg L-1 H2O2. Photosystem II quantum yield (photosynthetic activity) indicated cyanobacteria cell stress within 6 h, which was considerably faster than the other methods. Intracellular microcystins (MC-LR, -LY, -LW and -LF) were reduced by at least 96% after 24 h of H2O2 treatment. No increase in extracellular microcystin concentration was detected, which suggests that the intracellular microcystins released into the surrounding water were completely removed by the hydrogen peroxide. Thus, photosynthetic activity was deemed the most suitable and rapid method for oxidative cell stress detection in cyanobacteria, however, an approach using combined methods is recomended for efficient water treatment management.Industrial discharge of chromium (Cr) into environment puts serious threat on living beings due to its potent toxicity. Phytostabilization, a type of in-situ phytoremediation is aimed to immobilize and stabilize the toxic elements in soil using root system of metal resistant potential plants. To evaluate the phytostabilization potential of two grass species Brachiaria mutica and Leptochloa fusca, a pot study was conducted using soil spiked with different concentrations of Cr (control, 25, 50 and 100 mg kg-1). Three plants were sown in each pot with three replications and arranged following completely randomized design. After three months of growth, the plants were harvested and above and below ground plant's parts were analyzed for various growth and physiological parameters. Data revealed that plant biomass, chlorophylls and carotenoids reduced substantially with increasing Cr concentration. Antioxidant enzymatic activity increased significantly in L. fusca as compared to B. mutica with increasing Cr levels (up to 50 mg kg-1), then reduced at maximum Cr level (100 mg kg-1) in both grasses. Leptochloa fusca performed better with maximum root Cr accumulation 93.7 μg plant-1, shoot Cr accumulation 24.7 μg plant-1, root bioconcentration factor (BCF) 2.0, shoot BCF 0.08, shoot TF 0.06 and MTI 87%. While B. mutica showed maximum root Cr accumulation 18.4 μg plant-1, shoot Cr accumulation 7.6 μg plant-1, shoot BCF 0.03, root BCF 1.28, shoot TF 0.04, and MTI 56%. These results showed that L. fusca possessed good potential with better Cr bioaccumulation, MTI, BCF and antioxidant activities compared to B. mutica. Hence L. fusca can be used as good phytostabilizing agent for the soils contaminated with lower to moderate levels of Cr.Its high molecular weight endows benzo[a]pyrene (BaP) with strong adsorption to soil, causing serious soil contamination. Our previous study has reported that hemoglobin (Hb) is able to oxidize organic pollutants in the presence of H2O2. This present study showed that Hb catalytic mechanism for BaP oxidation was similar to that of lignin peroxidase. 2-Methyl-3-vinylmaleimide was confirmed as a major degradation intermediate of BaP by Hb catalysis. In addition, BaP was shown to be degraded by heme (Hm)-catalyzed reaction, suggesting that Hm of Hb is the essential catalytic center. selleck chemicals Rate constants (k) for BaP oxidation by Hm-catalyzed reaction were 0.4954 h-1. The major degradation intermediate by Hm-catalyzed reaction is 3,3',5,5'-tetramethylbiphenyl. While values of Km and Vmax of Hb and Hm are very similar, kcat values was 100 times higher with Hb than with Hm. But kcat value for Hb was much lower than that for lignin peroxidase H2. All the results above suggested that Hb-catalyzed reactions efficiently degrade BaP in aqueous condition. Thus, we suggest that Hb for oxygen carrier in blood could be employed as a biocatalyst (i.e., hemoglobin peroxidase) for BaP degradation in the environment, due to the high availability of Hb.