Ohlsenmohamad7117
HTR1B gene had significant genetic effects on milk fatty acids in dairy cattle.
In conclusion, our findings provided first evidence that the HTR1B gene had significant genetic effects on milk fatty acids in dairy cattle.
In Iraqi community, abnormal pregnancy form a major social ans psychological and health problem. The underlying etiology of this health phenomenon was varied and includes sets of infections and autoimmune diseases. Globally human parvovirus 19 infection is common and the infection attribute to bad obstetric outcomes. Global maternal parvovirus B19 remote infection rate was with a range of 13.2% to 97.9%, while the range of acute infection was 0.5% to 97.9%. Tanespimycin In Arab countries, the IgG seroprevalence was 53.3% to 74%, while IgM seroprevalence range was 2.2% to 84%.
To evaluate the role of Parvovirus B19 as an etiology of bad obstetric outcome in women in Kirkuk, Iraq.
Descriptive Case Control Study. Women included in the study were recruited from Kirkuk General Hospital and their age ranged from 14 to 48 years. A 663 women were included in the study and 237 of them were none pregnant, while the pregnant were 215. Additionally, the study included 211 women with inevitable abortion. A control group (306 womer in women with normal pregnancy (49.7%) than in those with inevitable abortion (64.9%), While IgG seroprevalence difference was not significant between the two groups. Infection seroprevalence was more frequent in housewife, uneducated women, large family size, non-smoker, rural area, non animal exposure, repeated abortion, congenital anomalies, and anaemia.
Parvovirus B19 infection may be with bad obstetric outcomes if occurred during pregnancy and OR confirm a significant association of the infection with parvovirus with smoking, occupation, crowding index, education, animal exposure and number of repeated abortion.
Parvovirus B19 infection may be with bad obstetric outcomes if occurred during pregnancy and OR confirm a significant association of the infection with parvovirus with smoking, occupation, crowding index, education, animal exposure and number of repeated abortion.Cancer is one of the major reasons for mortality across the globe. Side effects that are observed with the pharmacological medications present in the market majorly affect the quality of life of patients. This has caused the researchers to find an alternative source of medications such as herbal medicine which has shown a promising effect in anticancer treatment, one such source is Pomegranate, which belongs to the family Punicaceae. Several polyphenols are present in Punica granatum which exhibits properties ranging from antioxidant effect, antidiabetic effect, beneficial impact in treatment, and management of metabolic and cardiovascular disorders to advantageous impact in anticancer treatment. Polyphenols like punicalin, punicalagin, and ellagic acid are a few of the many compounds responsible for the anticancer activity of pomegranate. Many preparations of pomegranate such as Pomegranate Juice (PJ), Pomegranate seed oil (PSO), Pomegranate peel extract (PoPx) etc. are used in various clinical studies. These polyphenols show anticancer activity by either arresting the cell cycle in the G2/M phase, inducing apoptosis, or by damaging the DNA of tumor cells. This review explicitly discusses the role and mechanism of bioactives obtained from the pomegranate in the treatment and management of cancer. The chemical structure, properties and role of pomegranate in the treatment of breast, lung, thyroid, colon, and prostate cancer has been focused in detail. This review also discusses various drug delivery approaches for targeted delivery on tumors as well as patented preparation of pomegranate compounds along with the ongoing clinical trials.
The growing prevalence of bladder cancer worldwide has become a major concern for the researchers, and the side effects of chemotherapy drugs have always been a major problem in cancer treatment. Cinnamaldehyde, the active ingredient in the Cinnamon plant, has long been considered with anti-oxidant and anti-inflammatory effects.
Bladder cancer 5637 cell lines were treated with the different concentrations of Cinnamaldehyde. MTT assay was performed to evaluate cell viability at 24, 48, and 72 hours. The concentration of 0.02, 0.04, and 0.08 mg/ml of Cinnamaldehyde were selected. Apoptosis was assessed with Annexin V-FITC/PI and Hochest33258 staining. Cell migration was performed by the scratch test. To evaluate Cinnamaldehyde effect on glycolysis, the gene expression of epidermal growth factor receptor 2 (ErbB2), heat shock protein transcription factor-1 (HSF1) and lactate dehydrogenase A (LDHA), as well as the protein levels of HSF1 and LDHA, LDH activity and finally glucose consumption and lactate production, were measured.
Cinnamaldehyde significantly increased apoptosis rate in the 5637 cells (p<0.05). Furthermore, it significantly reduced the gene expression of ErbB2, HSF1, and LDHA, protein level of HSF1 and LDHA, LDH activity, as well as cell migration, glucose consumption, and lactate production (p<0.05). These changes were dose-dependent.
Thus, Cinnamaldehyde induced apoptosis and decreased growth in 5637 cells by reducing ErbB2-HSF1-LDHA pathway.
Thus, Cinnamaldehyde induced apoptosis and decreased growth in 5637 cells by reducing ErbB2-HSF1-LDHA pathway.
The impact of neoadjuvant therapy on long-term prognosis of patients with resectable rectal cancer is currently unknown.
This study aimed to explore the long-term prognosis of patients with resectable rectal cancer following treatment with neoadjuvant therapy.
Four major databases (PubMed, Web of Science, Embase, Cochrane library) were searched to identify relevant articles published between January 2000 and July 2020. The main outcome indicators were the 5-year overall survival (OS) and disease-free survival (DFS).
The meta-analysis revealed that 5-year OS (HR 0.88, 95% Cl 0.83-0.93) and DFS (HR 0.95, 95% Cl 0.91-0.98) were higher in patients with resectable rectal cancer after receiving neoadjuvant therapy than those treated with upfront surgery. Subgroup analysis demonstrated that the long-term survival of patients in Asia and Europe could benefit from neoadjuvant therapy. The neoadjuvant short-course radiotherapy (SCRT) and neoadjuvant chemo-radiotherapy (CRT) improved the 5-year OS and DFS of patients with stage Ⅱ-Ⅲ rectal cancer and mid/low rectal cancer. Further research found that patients with stage Ⅱ only had an increase in OS, while patients with stage Ⅲ have improved 5-year OS and DFS.
Neoadjuvant therapy improved the long-term survival of patients with mid/low rectal cancer in stage Ⅱ-Ⅲ (especially stage Ⅲ). Additionally, patients in Asia and Europe seemed to be more likely to benefit from neoadjuvant therapy. For the treatment, we recommend neoadjuvant SCRT and neoadjuvant CRT for resectable rectal cancer.
Neoadjuvant therapy improved the long-term survival of patients with mid/low rectal cancer in stage Ⅱ-Ⅲ (especially stage Ⅲ). Additionally, patients in Asia and Europe seemed to be more likely to benefit from neoadjuvant therapy. For the treatment, we recommend neoadjuvant SCRT and neoadjuvant CRT for resectable rectal cancer.
Copper complex has been gaining much attention in anticancer research as targeted agent since cancer cells uptake more copper than non-cancerous cells. Our group has synthesised a ternary copper complex which is composed of 1,10-phenanthroline and tyrosine [Cu(phen)(L-tyr)Cl].3H
0. These two payloads are designed to cleave DNA and inhibit protein degradation system (proteasome) concurrently in cancer cells, making this copper complex a dual-target compound.
Current study was carried out to investigate the mode of cell death and role of autophagy induced by [Cu(phen)(L-tyr)Cl].3H
0 in MCF-7 and MDA-MB-231 breast cancer cells.
Growth inhibition of [Cu(phen)(L-tyr)Cl].3H
0 towards MDA-MB-231 and human non-cancerous MCF10A breast cells was determined by MTT assay. Annexin-V-FITC/PI and cell cycle analysis were evaluated by flow cytometry. The expression of p53, Bax, caspase-9, caspase-7, caspase-3 and LC3 were determined using western blot analysis. The cells were then co-treated with hydroxychloroquine to ascertain the role of autophagy induced by [Cu(phen)(L-tyr)Cl].3H
0.
[Cu(phen)(L-tyr)Cl].3H20 inhibited the growth of cancer cells dose-dependently with less toxicity towards MCF10A cells. Additionally, [Cu(phen)(L-tyr)Cl].3H20 induced apoptosis and cell cycle arrest towards MCF-7 and MDA-MB-231 breast cancer cells possibly via regulation of p53, Bax, caspase-9, caspase-3 and capase-7. The expression of LC3II was upregulated in both cancer cell lines upon treatment with [Cu(phen)(L-tyr) Cl].3H
0, indicating the induction of autophagy. Co-treatment with autophagy inhibitor hydroxychloroquine significantly enhanced growth inhibition of both cell lines, suggesting that the autophagy induced by [Cu(phen)(L-tyr) Cl].3H
0 in both breast cancer cells was promoting cell survival.
[Cu(phen)(L-tyr)Cl].3H20 holds great potential to be developed for breast cancer treatment.
[Cu(phen)(L-tyr)Cl].3H20 holds great potential to be developed for breast cancer treatment.
Gold nanorods (GNRs) are very promising agents that have multiple applications in medicine and biology. However, the cytotoxic effects of GNRs have not been fully explored.
Therefore, the main objective of this study was to determine the selective cytotoxic effect of GNRs towards several human tumor cell lines.
To address this issue, three sizes of GNRs (10-nm, 25-nm, and 50-nm) were tested against two human tumor cell lines, namely, human hepatoma HepG2 and human prostate PC3 cancer cells. As GNRs are usually stored in soft tissues inside living bodies, we also tested the effect of GNRs on murine splenocyte viability. To determine if the GNRs displayed selectivity cytotoxicity towards cancer cells, active GNRs with the size showing the least cytotoxicity to splenocytes were then tested against a panel of 11 human tumor cell lines and two human non-tumor cell lines.
Our results showed that the most cytotoxic size of GNRs is 10-nm, followed by the 25-nm GNRs, while the 50-nm GNRs did not show a signifind human breast cancer cells (MCF7, MDA-MB-231, and MDA-MB-468 cells). The effect of GNRs was confirmed using the colony formation assay and the effect was found to be cell cycle specific. Finally, it was shown that laser treatment can potentiate the cytotoxic effect of the 25-nm GNRs.
GNRs are selective cytotoxic agents and they have the potential to act as candidate anticancer agents.
GNRs are selective cytotoxic agents and they have the potential to act as candidate anticancer agents.
This study aimed to appraise the activity of Pterocladia capillacea and Corallina officinalis polysaccharides against breast cancer stem cells (BCSCs). P. capillacea and C. officinalis polysaccharides were characterized to be sulfated polysaccharide-protein complexes.
Cytotoxicity of the polysaccharides against MDA-MB-231 and MCF-7 cell lines along with their impact on CD44+/CD24- and aldehyde dehydrogenase 1(ALDH1) positive BCSC population were determined. Their effect on gene expression of CSC markers, Wnt/β-catenin and Notch signaling pathways was evaluated.
P. capillacea and C. officinalis polysaccharides inhibited the growth of breast cancer cells and reduced BCSC subpopulation. P. capillacea polysaccharides significantly down-regulated OCT4, SOX2, ALDH1A3 and vimentin in MDA-MB-231 as well as in MCF-7 cells except for vimentin that was up-regulated in MCF-7 cells. C. officinalis polysaccharides exhibited similar effects except for OCT4 that was up-regulated in MDA-MB-231 cells. Significant suppression of Cyclin D1 gene expression was noted in MDA-MB-231 and MCF-7 cells treated with P.
