Bergerputnam7339

Another patient developed signs of shock and received continuous renal replacement. There were no deaths caused by AAP. Therefore, early identification of patients at risk of AAP is of great importance. In addition, repeated elevation in the levels of pancreatic enzymes is indicative of complications. Sanger sequencing analysis of ULK2 gene showed that there was a significant difference of EXON1 -493C>T and EXON1 -308C>G between the AAP group and control group (P less then 0.0001). Thus, ULK2 gene polymorphism may be associated with the development of AAP. However, more validation of this finding is needed. IJCEP Copyright © 2020.OBJECTIVE To investigate the effect of the AKT1 gene mutation hotspot E17K on the growth, proliferation, survival, and migration of breast cancer cells, based on the survival and prognosis of breast cancer patients with the AKT1 E17K mutation shown in TCGA database. METHODS The survival and incidence rates of AKT1 E17K mutation hotspots in breast cancer and other cancers were extracted from the Cancer Genome Atlas (TCGA). The recombinant eukaryotic expression plasmid AKT1 E17K-pIRES2-EGFP was constructed and transfected into breast cancer MCF-7, and MDA-MB-231 cell lines. MCF-7 and MDA-MB-231 cell lines were randomly divided into blank control groups, empty plasmid groups, and recombinant plasmid groups. The growth curve was drawn using the cell counting method. The proliferation and division of breast cancer cells were detected by CFSE fluorescent dye tracking. Apoptosis was detected by Annexin V/PI double labeling and cell vitality was detected using MTT assays, and cell migratory ability was detected by ceer patients with this mutation are good, which may be related to the inhibition of the PI3K/AKT/mTOR signaling pathway. IJCEP Copyright © 2020.Background Migraine is a common neurological disease, which burdens individuals and society all over the world. Acupuncture, an important method in Traditional Chinese Medicine, is widely used in clinical practice as a treatment for migraine. Several systematic reviews (SRs) have investigated the effectiveness and safety of acupuncture for migraine. Objective To summarize and critically assess the quality of relevant SRs and present an objective and comprehensive evidence on the effectiveness and safety of acupuncture for migraine. Data Sources. MEDLINE, Embase, Cochrane Library, PROSPERO database, Chinese National Knowledge Infrastructure (CNKI), Chinese Biological Medicine (CBM), China Science and Technology Journal (SCTJ), and WanFang database (WF) were searched from inception to December 2019 and grey literatures were manually searched. Selection Criteria. SRs which meet the criteria were independently selected by 2 reviewers according to a predetermined protocol. Data Extraction. Characteristics of incluor to western medicine in treatment of migraine. Besides, acupuncture reduced more headache days and the times of using painkiller and was more effective in reducing the frequency and degree of headache than western medicine and sham acupuncture. Limitations. There might be some missing information. The accuracy of the conclusions may be decreased reduced since we were unable to synthesis all the evidence. Conclusions Based on high quality of evidence, we concluded that acupuncture may be an effective and safe therapy for migraine. However, the quality of SRs in acupuncture for migraine still needs more improvement. Copyright © 2020 Yu-Xi Li et al.This study aimed to examine the interchangeability of two external training load (ETL) monitoring methods arbitrary vs. individualized speed zones. Thirteen male outfield players from a professional soccer team were monitored during training sessions using 10-Hz GPS units over an 8-week competitive period (n = 302 observations). Low-speed activities (LSA), moderate-speed running (MSR), high-speed running (HSR) and sprinting were defined using arbitrary speed zones as less then 14.4, 14.4-19.8, 19.8-25.1 and ≥25.2 km·h-1, and using individualized speed zones based on a combination of maximal aerobic speed (MAS, derived from the Yo-yo Intermittent recovery test level 1), maximal sprinting speed (MSS, derived from the maximal speed reached during training) and anaerobic speed reserve (ASR) as less then 80% MAS, 80-100% MAS, 100% MAS or 29% ASR and ≥30% ASR. Distance covered in both arbitrary and individualized methods was almost certainly correlated in all speed zones (p less then 0.01; r = 0.67-0.78). However, significant differences between methods were observed in all speed zones (p less then 0.01). LSA was almost certainly higher when using the arbitrary method than when using the individualized method (p less then 0.01; ES = 5.47 [5.18; 5.76], respectively). Conversely, MSR, HSR and sprinting speed were higher in the individualized method than in the arbitrary method (p less then 0.01; ES = 5.10 [4.82; 5.37], 0.86 [0.72; 1.00] and 1.22 [1.08; 1.37], respectively). Arbitrary and individualized methods for ETL quantification based on speed zones showed similar sensitivity in depicting player locomotor demands. However, since these methods significantly differ at absolute level (based on measurement bias), arbitrary and individualized speed zones should not be used interchangeably. © 2020 Vincenzo Rago, João Brito, Pedro Figueiredo, Peter Krustrup, António Rebelo, published by Sciendo.We aimed to create a single subjective method to assess both internal training loads and subsequent fatigue. This new training-fatigue (dose-response) scale (TFS) was composed of two similar scales with the same properties, metrics and construction criteria. These two scales were designed to rate the perceived exertion (RPETFS) and perceived fatigue (RPFTFS) in professional soccer players. Twenty-two athletes participated to establish reliability, and 15 participated to establish validity. For reliability, the intraclass correlation coefficient (ICC) and standard error of measurement (SEM) were used. For criterion validity, the Spearman's correlation coefficient and linear regression analyses were applied. Associations between RPETFS and RPFTFS were verified by a chi square test, and a further factorial exploratory analysis was conducted. RPETFS and RPFTFS were found to be reliable (ICC 0.74 and 0.77, SEM 0.30 and 0.30, respectively) and valid. RPETFS was best explained by the internal load of the Banister training impulse (p less then 0.001), while RPFTFS was best explained by the internal load of the Stagno training impulse (p less then 0.001). An association was found between the scales (RPETFS and RPFTFS) in which training duration had a more substantial impact on these subjective perceptions than did training intensity (p less then 0.01). RPETFS and RPFTFS scales are reliable and valid for monitoring training sessions in Brazilian professional soccer players. The simultaneous oscillations of the RPETFS and RPFTFS scores can be used by staff members to better plan weekly training programs based on dose-response ratings. Finally, training duration must be carefully controlled because it has a greater impact than intensity on subjective perceptions. © 2020 Rodrigo F. Morandi, Eduardo M. Pimenta, André G. P. Andrade, Tane K. F. Serpa, Eduardo M. Penna, Charles O. Costa, Mário N. S. O. Júnior, Emerson S. Garcia, published by Sciendo.This study aimed to describe the physical and physiological demands of adolescent handball players and compare movement analysis and exercise intensities between the first and second halves and between the different periods of the match. Fourteen adolescent handball players (age 15.7 ± 0.8 years, body mass 65.6 ± 3.4 kg, body height 169.5 ± 3.9 cm), played two friendly matches, in which no substitutions were made. The analysis was carried out with a Global Positioning System technology. The following physical variables were analyzed Total distance covered (TD); distance covered at faster velocities than 18 km·h-1 (TDC>18km·h-1); number of accelerations (Accel) and decelerations (Decel); number of accelerations and decelerations higher than 2.78 m·s-2 (Accel>2.78 m·s-2 and Decel>2.78 m·s-2); number of sprints (Sprints); accelerations interspersed with a maximum of 30 s between them (RAS≤30s) and as a physiological variable the heart rate (HR) was examined. Significant differences (p 2.78 m·s-2. click here This trend was also observed when comparing performance between the different 10-min periods. The 5th period (period 40-50 min) was the one that showed differences with respect to the previous ones. Adolescent handball players showed lower levels of exercise intensity, assessed by both time-motion and HR data, in the second half of matches, especially in the middle of this period. © 2020 Manuel Ortega-Becerra, Alexis Belloso-Vergara, Fernando Pareja-Blanco, published by Sciendo.The aim of the study was to compare motor action variables of judo combat phases and technical biomechanical assessment of the seven weight categories. The sample was composed of 638 bouts (176 of extra lightweight 100 kg) during motor actions of approach, gripping, attack, defense and groundwork combat phases, verifying the interactions between them by Markov chains and comparisons by Kruskall-Wallis and Dunn post hoc tests (p ≤ .05). The results demonstrated that lighter weight categories showed higher approach phases than the heavyweight category, and the lightweight group demonstrated higher defensive action frequencies than the half lightweight category and heavyweight athletes. Heavyweight and middleweight categories demonstrated higher groundwork combat frequencies than lighter weights, and the heavyweight category showed higher pause frequencies than the lightweight category. Regarding the decision-making model, the main transitions presented by the Markov chains showed higher prevalence of the following combinations gripping occurring before the lever and length attacks, lever attack followed by the length attack, and length attack occurring earlier than the groundwork phase. © 2020 Dany Alexis Sobarzo Soto, Esteban Aedo-Muñoz, Ciro José Brito, Suzi Camey, Bianca Miarka, published by Sciendo.The aims of the study were a) to analyze the reproducibility of the Modified Agility Test (MAT) according to two types of displacement (i.e. constrained [MATtop] vs. free [MATfree]), b) to examine the explanatory capacity of anthropometric characteristics and neuromuscular performance on the ability to change the direction (CODA), c) to look into the practical consequences of the types of displacement from the perspective of an elite soccer academy. 118 male soccer players (age 16 (13-25) years old) from the same elite Spanish soccer academy (U13 to senior) were tested twice on two versions of the MAT (MATtop and MATfree), with 48 hours between testing sessions. Moreover, they were tested on linear-sprint performance, over 5 m (S5m) and 15 m (S15m), and the vertical jump (VJ) (countermovement jump with [ACMJ] and without an arm swing [CMJ]). The main findings were a) the type of displacement did not affect the reliability of the CODA test; b) weight, S15m, ACMJ and CMJ variables explained close to 60% of CODA performance; c) MATtop (i.e. constrained displacement) and MATfree (i.e. free-displacement) CODA tests could show different profiles of development along the age groups; and d) the impact of the task's constraints was relatively higher in U16 and U17 groups. CODA seems to have a variable meaning depending on the characteristics of the test and the age of the participants. © 2020 Asier Los Arcos, José F. Aramendi, Jose I. Emparanza, Carlo Castagna, Javier Yanci, Alejandro Lezáun, Raúl Martínez-Santos, published by Sciendo.