Bondesentillman6459

Ecotoxicological effects of psychiatric drugs and drug metabolites released by the human population are of increasing environmental concern. In this study we evaluate behavioral responses to visual predator cues in wild caught three-spined stickleback (Gasterosteus aculeatus) after exposure to water-born citalopram, a widely prescribed selective serotonin reuptake inhibitor with antidepressant and anxiolytic effects. Fish were exposed to ecological relevant concentrations of citalopram (0.15 or 1.5 μg L-1) for 10 or 20 days. After drug exposure, individual fish were moved to a test arena where they were exposed to two naturalistic visual predator cues; a shadow from beneath, which simulated an approaching fish, and an overhead silhouette of a passing gull. Both visual cues resulted in decreased locomotor activity after post cue presentation. Notably, citalopram exposure resulted in a dose dependent suppression in response to the overhead stimulus. These results show that an ecologically relevant stimulus elicits a robust avoidance behavioral in wild caught fish after 25 min of acclimatization in the test arena. This suggests that the gull stimulus can be utilized as a behavioral endpoint in high flow through assays of ecotoxicological effects of psychiatric drugs and drug metabolites. Furthermore, the short acclimation time of wild caught fish in the test arena, opens for behavioral screening by fish living or kept in water bodies which are potentially impacted by psychiatric drugs.Wetlands play a vital role in removing nitrogen (N) from aquatic environments via the denitrification process, which is regulated by multiple environmental and biological factors. Until now, the mechanisms by which environmental factors and microbial abundance regulate denitrification rates in wetlands under different hydrological conditions remain poorly understood. Here, we investigated sediment potential denitrification rate (PDR) and unamended denitrification rate (UDR), and quantified denitrifier abundance (nirS, nirK, and nosZ genes) in 36 stream, river, pond, and ditch wetland sites along the Dan River, a nitrogen-rich river in central China. The result indicated that ditches had the highest denitrification rates and denitrifier abundance. Both PDR and UDR showed strong seasonality, and were observed to be negatively correlated with water velocity in streams and rivers. Moreover, denitrification rates were significantly related to denitrifier abundance and many water quality parameters and sediment properties. Interestingly, PDR and UDR were generally positively associated with N and carbon (C) availability in streams and rivers, but such correlations were not found in ponds and ditches. Using a scaling analysis, we found that environmental parameters, including Reynolds number, sediment total C ratio, and interstitial space, coupled with relative nirS gene abundance could predict the hotspots of denitrification rates in wetlands with varying hydrologic regimes. Our findings highlight that hydrological conditions, especially water velocity and hydrologic pulsing, play a nonnegligible role in determining N biogeochemical processes in wetlands.Antimony (Sb), as a newly identified nerve poison, can lead to neuronal apoptosis. However, its neurotoxicological mechanisms remain largely unclear. Here, we evaluated the role and regulation of Wnt/β-catenin pathway in Sb-mediated neurotoxicity. Under Sb treatment, β-catenin was dramatically downregulated in vivo and in vitro. Moreover, overexpression of β-catenin effectively attenuated Sb-induced survivin gene expression suppression and subsequent apoptosis in PC12 cells. In addition, Sb stimualted glycogen synthase kinase-3β (GSK-3β) activation, shown as decreased phosphorylation levels at Ser 9 both in PC12 cells and mice brain. Paramacological inhibition of GSK-3β using lithium chloride (LiCl) significantly rescued β-catenin expression. For upstream pathway analysis, we found Sb treatment decreased protein kinase B (Akt) phosphorylation, and Akt activator protected PC12 cells from GSK-3β activation and subsequent β-catenin suppression. In summary, our data provided a novel molecular mechanism of Sb-associated neurotoxicity, namely that Sb induces Wnt/β-catenin pathway suppression through Akt inhibition, thus resulted in neuronal apoptosis.Forest fragments in urban parks provide important habitat for birds. However, the guano deposited by large aggregations of birds in such fragments can dramatically change soil properties, which in turn, can alter soil microbial community composition, potentially affecting the forests' composition and survival. To investigate the effects of bird aggregations on the soil of fragmented urban forests, we compared the soil properties and microbial communities of two forested islands, one in Liuhuahu park and the other in Wanzuitou park, Guangzhou, where large numbers of birds aggregate yearly to nest. Comparison to sites without bird aggregations suggests that decades of guano deposition appears to have caused soil acidification and an increase in soil nutrients. The relative abundance of the soil bacterial phylum Actinobacteria and the soil fungal phylum Ascomycota were significantly lower in soil under bird aggregations. The aerobic nitrite oxidation, nitrate reduction and cellulolysis bacterial guilds were significantly less abundant under bird aggregations in Liuhuahu park. The wood saprotroph fungi guild was significantly less abundant under the bird aggregation in Liuhuahua park and the pathogenic fungi guild significantly more abundant in Wanzuitou park. Soil properties, including TN, NO3--N, TOC and pH, explained the variation in Ascomycota and Basidiomycota abundance, and the alpha-diversity of the fungal community. Microbial community variation could potentially slow the rate of decomposition and disease resistance of plant in these forests. We suggest that sufficient contiguous forest should be maintained in urban areas to reduce the density of bird aggregations in isolated forest fragments.Zinc (Zn) fertilizer application can certainly improve the production and nutritional quality of cereal crops. selleck inhibitor However, Zn accumulation in the soil may lead to some deleterious environmental impacts in agroecosystems. The effects of long-term Zn application on soil microbial properties remain unclear, but it is imperative to understand such effects. In this study, we collected soil samples from a nine-year field experiment in a wheat-maize system that continuously received Zn applied at various rates (0, 2.3, 5.7, 11.4, 22.7 and 34.1 kg ha-1) to evaluate the soil enzymes, microbial biomass and microbial community structure. The results showed that Zn application at the rate of 5.7 kg ha-1 significantly increased the activities of urease, invertase, alkaline phosphatase and catalase in the soil, while the rate of 34.1 kg ha-1 significantly decreased the evaluated enzyme activities. The microbial biomass carbon (C) and nitrogen (N) were not affected by Zn application rates, although an increase in the microbial biomass C was observed in the 11.