Hardyhalberg1264

Both plastic additives such as PBDEs, and chemical contaminants adsorbed from the environment such as PCBs and DDE, were analyzed in the EMP, feed and liver. The concentration of microplastics in the feed was calculated based on the MPs/zooplankton wet weight (WW) ratio of 0.1 found in an area of maximum accumulation in the Canary Islands. Therefore, it is an experiment that simulates real conditions, but in the worst-case scenario, using both, concentrations based on data obtained in oceanographic campaigns and microplastics collected from the environment. Our results show that in this scenario, additives and chemical contaminants adsorbed on EMPs bioaccumulate in fish liver due to long-term ingestion of microplastics.The effects of membrane permeability on extracellular electron transfer (EET) and performance of microbial fuel cell (MFC) need to be explored. In this work, cetyltrimethylammonium bromide (CTAB) was chosen to enhance the current generation and bidirectional transport of substrate and electron shuttles by tailoring the cell membrane permeability. Specifically, the peak currents of biofilms treated with CTAB especially at 200 μM were obviously higher than the control biofilm with no CTAB, and the riboflavin mediated electron transfer was promoted prominently. Biomass and viability analyses showed that an appropriate concentration of CTAB had almost no adverse effect on the cell viability of biofilm and could increase the biomass of biofilm. Measurements of the extracellular activity of alkaline phosphatase and UV-vis absorption confirmed the increased membrane permeability and the promoted efficiency of substrates transported into cells. This contribution paves the key step for facilitating EET process by adjusting membrane permeability through CTAB or other surfactants addition.Biochar has been widely studied as a soil amendment, but little is known about the "biochar-freeze-thaw soil-crop root system" interface in seasonally frozen soil areas. In the second year after the application of biochar, we conducted research on the morphological characteristic indicators of the soybean root system and the nutrient migration of the soil in the root zone under different biochar application periods (spring and autumn mixed, autumn, and spring biochar application) and different biochar application rates (3 kg·m-2, 6 kg·m-2, 9 kg·m-2, and 12 kg·m-2). The effects of different biochar treatments on the growth and development of soybean roots were examined. The soil organic carbon, ammonium nitrogen and nitrate nitrogen contents of the soil were measured at different locations in the root zone, and the migration processes of these nutrients in the soil were explored. The conclusions drawn from the experiments are as follows. Pidnarulex cost (i) The biochar application rate and application method together determine the root morphological characteristic indicators of soybean plants. During long freeze-thaw periods, the freeze-thaw cycles change the internal environment of the biochar-freeze-thaw soil complex. (ii) Biochar tends to move towards the root system, which can increase soil organic carbon content, but the effect of biochar on root characteristics is not caused by the change in soil organic carbon content. (iii) Biochar promotes nitrogen cycling in the soil and the migration of soil nitrogen to the root sheath, increasing the number of nitrogen compounds that can be directly absorbed and utilized by crops. (iv) From a comparison of the effects of various biochar treatments on crop roots and farmland soils, we suggest that the 9 kg·m-2 biochar application rate under spring and autumn mixed biochar application is the optimal treatment.The broad-spectrum insecticide p,p'-dichlorodiphenyltrichloroethane (p,p'-DDT) has been banned in most countries since the 1970s on account of its environmental persistence as well as the high biomagnification of its major metabolite 1,1-dichloro-2,2-bis(4-chorophenyl)ethylene (p,p'-DDE). However, the information on the bioaccumulation and behavior of p,p'-DDTs in aquatic organisms is lacking. In this study, all 6 DDT isomers were detected in biota from the food web of the Liaodong Bay, China, and the total concentrations of DDT isomers in Chinese anchovy (Thrissa kammalensis) and Japanese Spanish mackerel (Scomberomrus niphonius) were 223 ± 42 ng/g ww and 242 ± 70 ng/g ww, respectively. In biota, o,p'-DDD dominated among the o,p'-isomers (80.5 ± 17.3%), while p,p'-DDE dominated among the p,p'-isomers (61.8 ± 15.2%). Contrastingly, sediment from the Liaodong Bay contained similar proportions of o,p'-DDT and p,p'-DDTs, suggesting an isomer-specific metabolism of the compounds in biota. A well-controlled laboratory exposure experiment with Japanese medaka (Oryzias latipes) demonstrated that o,p'-DDT was more difficult to metabolize to o,p'-DDE compared with that of p,p'-DDT. Significantly positive regressions were found between trophic levels and lipid equivalent concentrations for both o,p'-DDT and o,p'-DDD, and the trophic magnification factors (TMFs) were estimated as 12.3 and 9.12 (p less then 0.05), respectively. The TMFs of o,p'-DDT and o,p'-DDD in the aquatic food web were higher than p,p'-DDT (7.76), p,p'-DDD (4.17), and p,p'-DDE (3.39), which may be explained by the isomer-specific metabolism differences in biota.In this work, the evaluation of the life cycle of the service provided by a medium-power motorcycle in a Spanish urban environment was carried out, comparing two motorcycles, a battery electric vehicle (BEV) is compared with an internal combustion engine vehicle (ICEV). The economic study of the total costs of ownership is also carried out considering the environmental costs associated with each type of vehicle. A comprehensive inventory is compiled for both vehicles (motorcycles) that describes the most relevant components and includes two types of batteries for the BEV. A sensitivity analysis of the most impactful parameters is also considered. The results indicate that the ICEV contributes approximately 5 times more in the global warming potential impact category mainly due to the consumption of fossil fuels. The BEV also impacts some categories in the manufacturing stage, a fact that is strongly related to the battery. Sensitivity analysis indicates that the total distance travelled plays an important role, but the electricity mix is probably the most relevant factor in terms of climate change impact category. The economic analysis reported lower environmental externality costs for the BEV, making it more affordable than the ICEV and highlighting the benefit in terms of air pollution. The BEV is presented as a suitable option vehicle from environmental and economic point of view and one of the actors to accelerate the transition towards a more sustainable urban mobility model.Methylphenidate (MPH) is a central nervous system (CNS) stimulant known for its effectiveness in the treatment of Attention Deficit Hyperactivity Disorder (ADHD), a neuropsychiatric condition that has a high incidence in childhood and affects behavior and cognition. However, the increase in its use among individuals who do not present all the diagnostic criteria for ADHD has become a serious public health problem since the neurological and psychiatric consequences of this unrestricted use are not widely known. In addition, since childhood is a critical period for the maturation of the CNS, the high prescription of MPH for preschool children also raises several concerns. This review brings new perspectives on how MPH (in different doses, routes of administration and ages) affects the CNS, focusing on animal studies that evaluated changes in mitochondrial (bioenergetics), redox balance and apoptosis, as well as inflammatory parameters. MPH alters brain energy homeostasis, increasing glucose consumption and impairing the activity of enzymes in the Krebs cycle and electron transport chain, as well as ATP levels and Na+,K+-ATPase activity. MPH induces oxidative stress, increasing the levels of reactive oxygen and nitrogen species and altering enzymatic and non-enzymatic antioxidant defenses, which, consequently, is related to damage to proteins, lipids, and DNA. Among the harmful effects of MPH, studies also demonstrate its ability to induce inflammation as well as alter the apoptosis pathway. It is important to highlight that age, treatment time, administration route, and dose are factors that can influence MPH effects. However, young animals seem to be more susceptible to damage caused by MPH. It is possible that changes in mitochondrial function and markers of status oxidative, apoptosis and inflammation may be exerting important mechanisms associated with MPH toxicity and, therefore, the unrestricted use of this drug can cause brain damage.

Food allergy and acute anaphylaxis can be life-threatening. While T follicular helper (Tfh) cells play a pivotal role in the allergic immune responses, the immunologic mechanisms that regulate the production of antibodies (Abs) that mediate anaphylaxis are not fully understood.

The aim of this study was to investigate the role of the inhibitory receptor programmed cell death protein 1 (PD-1), which is highly expressed on Tfh cells, in allergic immune responses using an animal model of peanut allergy and anaphylaxis.

Naive wild-type mice were exposed to peanut flour intranasally and then challenged with peanut extract to induce systemic anaphylaxis. The roles of PD-1 were examined by blocking Abs and using gene-deficient animals. Ahapten model and passive cutaneous anaphylaxis were used to characterize allergen-specific Abs.

Treatment with anti-PD-1 enhanced development of Tfh cells and germinal center B cells in mice exposed to peanut flour. Nonetheless, anti-PD-1 or its ligand fully protected mice from developing anaphylaxis. Anti-PD-1 treatment or genetic deficiency of PD-1 in CD4

T cells inhibited production of peanut-specific IgE and increased the levels of IgG. The passive cutaneous anaphylaxis showed that peanut-specific Abs generated in anti-PD-1-treated animals prevented, rather than provoked, anaphylaxis when transferred to naive animals. Anti-PD-1 promoted production of Abs with low affinity for an antigen in the hapten model.

Blockade of the pathway between PD-1 and its ligand is protective against allergic immune responses. The direct interaction between Tfh cells and B cells may play a pivotal role in controlling Ab quality and clinical manifestation of allergic diseases.

Blockade of the pathway between PD-1 and its ligand is protective against allergic immune responses. The direct interaction between Tfh cells and B cells may play a pivotal role in controlling Ab quality and clinical manifestation of allergic diseases.

Pancreatic cancer remains one of the most devastating malignancies due to the absence of techniques for early diagnosis and the lack of target therapeutic options for advanced disease. Next Generation Sequencing (NGS) generates high throughput and valuable genetic information when evaluating circulating tumor DNA (ctDNA); however clinical utility of liquid biopsy in pancreatic cancer has not been demonstrated yet. The aim of this study was to evaluate whether results from a Next Generation Sequencing panel on plasma samples from pancreatic cancer patients could have a clinical significance.

From December 2016 to January 2020, plasma samples from 27 patients with pancreatic ductal adenocarcinoma at two different tertiary Spanish Hospitals underwent ctDNA testing using a commercial NGS panel of 65 genes. Clinical data were available for these patients. VarsSome Clinical software was used to analyse NGS data and establish pathogenicity.

Evaluable NGS results were obtained in 18 out of the 27 plasma samples.