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The MMP-2 gene was hypermethylated, but there was no significant difference (p = 0.596) between peritoneal endometriosis tissue and endometrial tissue in terms of methylation. This study did not show a significant correlation between mRNA expression and DNA methylation levels of the MMP-2 gene (p = 0.769, r = 0.070).
The increase in MMP-2 gene expression in peritoneal endometriosis tissue is likely not only due to hypermethylation; there are other factors that might play a role.
The increase in MMP-2 gene expression in peritoneal endometriosis tissue is likely not only due to hypermethylation; there are other factors that might play a role.
Malnutrition is quite prevalent in hospitalized patients, and its diagnosis is particularly important for doctors in order to treat it accordingly. This study aims in finding the frequency of malnutrition in ambulatory hospitalized patients based on three international criteria.
The subjects of this study were hospitalized patients at Cipto Mangunkusumo Hospital, Jakarta, Indonesia. selleck chemicals llc The subjects were investigated for malnutrition using World Health Organization (WHO), American Society for Parenteral and Enteral Nutrition (ASPEN), and the European Society for Clinical Nutrition and Metabolism (ESPEN) criteria for malnutrition. Two hundred and twenty-nine ambulatory hospitalized patients were subject to accurate anthropometry measurements. They were analyzed for their height using Seca-360 and their weight using Seca-869, from which their body mass index could be measured. They were also examined for their handgrip strength using Jamar® Handgrip Dynamometer. The rest of the criteria were fulfilled through sly according to its guidelines (with the use of a validated instrument).
Renal function of patients with chronic kidney disease (CKD) is typically evaluated by detecting proteinuria because it is a major predictor of CKD progression. In paediatric patients with CKD, urine albumin-to-creatinine ratio (ACR) is used to detect CKD progression, which is similar to urine protein-to-creatinine ratio (PCR). However, facilities for evaluation of urine ACR and urine PCR may not be widely available. To date, this is the first study that investigated the predictive value of baseline dipstick albuminuria for 1-year and 3-year CKD progression in Indonesian children. We assessed the association between baseline level of dipstick albuminuria and CKD progression in paediatric patients.
This retrospective cohort study was conducted at the Cipto Mangunkusumo Hospital (CMH) involving 43 children with CKD between 2016 and 2019. The patients were followed up for 1 year and 3 years after enrolment. Risk ratios (RR) for 1-year and 3-year CKD progression were calculated using Fisher's exact test.
The RR for 1-year CKD progression in children with baseline dipstick albuminuria <2+ was 2.16 (95% CI 1.13-4.14, p = 0.02), and the corresponding RR for 3-year CKD progression in these children was 1.70 (95% CI 0.73-3.97, p=0.21).
Dipstick albuminuria was not associated with 1-year and 3-year CKD progression in children.
Dipstick albuminuria was not associated with 1-year and 3-year CKD progression in children.
This study aimed to evaluate the association between protein intake and number of children in a family, based on nutritional status of children aged 2 to 12 years of age.
This was a cross-sectional study with consecutive sampling. Weight and height were measured, and the 24-hour food recall was gathered with a questionnaire. Nutritional status was assessed using weight-for-age curve of Centers for Disease Control and Prevention (CDC) growth chart. Protein intake was assessed using NutriSurvey. Data were analysed with chi-square test.
There were 99 children, 52 girls and 47 boys. From these, 57 (57.6%) were undernourished and 42 (42.4%) subjects had a good nutritional status. Sixty-five (65.6%) children had poor protein intake, while 34 (34.3%) had adequate protein intake. There was no association between protein intake and nutritional status (p = 0.805) or number of children in the family and nutritional status (p = 0.414). The principal protein source was squid, but squid is often sold for additional income.
The nutritional status of children in Pero Konda Village could be considered undernourished. There was no association between protein intake and number of children based on nutritional status. The villagers had poor dietary habits, so good dietary habits should be promoted.
The nutritional status of children in Pero Konda Village could be considered undernourished. There was no association between protein intake and number of children based on nutritional status. The villagers had poor dietary habits, so good dietary habits should be promoted.
Infertility in women could be a result of an excessive production of antisperm antibody (ASA). Paternal lymphocyte immunization (PLI) could decrease the ASA levels, but the mechanism is still unclear. The aim of this study was to analyse the impact of a PLI-induced ASA decline on regulatory T-cell populations and serum interleukin-10 (IL-10) levels in women with unexplained infertility.
Samples were obtained from patients who came to Sayyidah Mother and Child Hospital in Jakarta from July 2018 to April 2019 with infertility problems. The inclusion criterion for this study was unexplained infertility. Each patient was examined for ASA titres using husband's sperm auto-agglutination test (HSAaT) method, and patients with ASA titres >1128 were given PLI subcutaneously every 3 weeks. ASA titres were evaluated again 2 weeks after PLI with HSAaT. A total of 12 samples were analysed. Regulatory T-cell populations were evaluated using flow cytometry and human forkhead box P3 FoxP3 staining kit of Biotech and Device, and serum IL-10 was determined using an Abcam ELISA kit. The data were analysed using Wilcoxon and Spearman tests.
PLI decreased serum ASA and percentage of regulatory T cells (p = 0.023). The decrease in ASA and subsequent decrease in regulatory T cell population was due to the strong negative correlation between regulatory T cells and IL-10 (r = -0.817, p = 0.004).
The decline in ASA was associated with a decrease in regulatory T cells due to a negative correlation with IL-10levels.
The decline in ASA was associated with a decrease in regulatory T cells due to a negative correlation with IL-10levels.
