Lawsonbendtsen6584

4 K-0.5 K) over a wide temperature sensing range of 300 K to 565 K, which are remarkably better than those of many other luminescence thermometers. This phosphor exhibits strong NIR emission at low excitation density, meaning that it has potential uses in deep tissue imaging, optical signal amplification and other fields. The results indicate that Tm3+/Yb3+NGVO is an ideal candidate for thermometers and particularly for biological applications.Electrode-based impedance and electrochemical measurements can provide cell-biology information that is difficult to obtain using optical-microscopy techniques. Such electrical methods are non-invasive, label-free, and continuous, eliminating the need for fluorescence reporters and overcoming optical imaging's throughput/temporal resolution limitations. Nonetheless, electrode-based techniques have not been heavily employed because devices typically contain few electrodes per well, resulting in noisy aggregate readouts. Complementary metal-oxide-semiconductor (CMOS) microelectrode arrays (MEAs) have sometimes been used for electrophysiological measurements with thousands of electrodes per well at sub-cellular pitches, but only basic impedance mappings of cell attachment have been performed outside of electrophysiology. Here, we report on new field-based impedance mapping and electrochemical mapping/patterning techniques to expand CMOS-MEA cell-biology applications. The methods enable accurate measurement of cell attachment, growth/wound healing, cell-cell adhesion, metabolic state, and redox properties with single-cell spatial resolution (20 μm electrode pitch). These measurements allow the quantification of adhesion and metabolic differences of cells expressing oncogenes versus wild-type controls. The multi-parametric, cell-population statistics captured by the chip-scale integrated device opens up new avenues for fully electronic high-throughput live-cell assays for phenotypic screening and drug discovery applications.Flexible, substrate-free nanowire (NW) devices are desirable to overcome the extremely challenging task of integrating III-V or III-N semiconductor devices such as LEDs and lasers on a range of optoelectronic circuits or biochips. In this work, we report the demonstration of core-shell p-InP/n-ZnO heterojunction NW array LEDs. The emission from the devices consists of three peaks at room temperature due to conduction band-to-heavy hole band transition, conduction band-to-light hole band transition and recombination at the substrate. At 78 K, an additional peak due to Zn acceptor levels is observed, whereas the peak due to the conduction band-to-light hole band transition quenches. Flexible LEDs are then fabricated by embedding the NW arrays in SU-8 to enable subsequent lift-off from the substrate. Compared with the original on-substrate LED device, broader, red-shifted and multiple peaks are observed from the flexible devices, which may be due to non-uniform strain related effects in the NWs caused by the SU-8 film. A slightly higher series resistance as compared to the on-substrate device and significant Joule heating suggest that good heatsinking is required for these flexible devices. Nevertheless, our study paves a promising way towards flexible and low power LEDs.A convenient and ultrasensitive ratiometric fluorescent probe was innovatively developed for Hg(II) detection and trypsin activity evaluation based on carbon dots (CDs) and tetraphenylporphyrin tetrasulfonic acid (TPPS) using bovine serum albumin (BSA) as the substrate of trypsin. The ratiometric fluorescence signal arises from CDs (λem = 506 nm) and TPPS (λem = 645 nm) via an inner filter effect. Hg2+ can trigger the formation of TPPS-Mn2+ metalloporphyrin for target Hg2+ recycling amplification, while both TPPS-Hg2+ and TPPS-Mn2+ metalloporphyrins do not affect the fluorescence of CDs. Small amino acids and peptide fragments, which are the products of BSA under the digestion of trypsin, bind stronger with Hg2+ than with TPPS. The decomposition of both TPPS-Hg2+ and TPPS-Mn2+ metalloporphyrins leads to a variation in the ratiometric fluorescence signal. Under optimized conditions, this probe provided an inspiring detection limit of 0.086 nM for Hg2+ and 0.013 ng mL-1 for trypsin, which possessed acceptable sensitivity for Hg2+ detection and trypsin activity evaluation in authentic samples. This unprecedented CD-based ratiometric fluorescence proposal for ultrasensitive quantification of Hg2+ concentration and selective assessment of trypsin activity gives a new insight for designing metal ion assays or enzymatic activity bioassays under different enzymatic substrates in the near future.We report a facile strategy for synthesizing uniform heterometallic bi/tri-atom clusters starting from mono-metallic atoms in the liquid phase. Specifically, Pt1,2Cu bi/tri-atoms are prepared by reducing CuCl2 at preformed Pt1 atoms with ethanol inside a PDMS-PEG protective layer. The metal atoms in the Pt1,2Cu clusters are in reduced chemical states.Detecting nitroreductase (NTR) activity in hypoxic cells and tissues in situ represents an important step toward accurate delineation of hypoxic disease loci. However, it remains challenging to develop fluorescent probes with the necessary attributes of selectivity, sensitivity, precise targeting and aqueous solubility. Herein, two kinds of fluorescent probes (NNP and cRGD-NNP) built on a 2-nitroimidazole sensing platform were synthesized for the detection of NTR activity in cell and in vivo models of hypoxia. In the presence of NADH, NNP displayed high selectivity for NTR, a strong fluorescence enhancement (108 fold), and a low detection limit (3.6 ng mL-1). Benefiting from the hydrophilic structure and tumor-targeting properties of the cRGD cyclopeptide group, the probe cRGD-NNP efficiently detected NTR activity in MCF cancer cells under hypoxia. In addition, the liposome-encapsulated probe was successfully applied to visualize NTR during liver inflammation in mice.

To identify the differentially expressed proteins in different liver tissues in the mouse model of cystic echinococcosis (CE), so as to provide insights into the research and development of therapeutic drugs targeting CE.

Female Kunming mice at ages of 6 to 8 weeks were randomly assigned into the CE group and the control group. Mice in the CE group were intraperitoneally infected with 2 000

protoscoleces, while mice in the control group were injected with the same volume of physiological saline. All mice in both groups were sacrificed after breeding for 350 d, and the lesions (the lesion group) and peri-lesion specimens (the peri-lesion group) were sampled from the liver of mice in the CE group and the normal liver specimens (the normal group) were sampled from mice in the control group for data independent acquisition (DIA) proteomics analysis, and the differentially expressed proteins were subjected to Gene Ontology (GO) term enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathifferentially expressed proteins are identified in the liver specimens between mouse models of CE and normal mice, and some differentially expressed proteins may serve as potential drug targets for CE.

Differentially expressed proteins are identified in the liver specimens between mouse models of CE and normal mice, and some differentially expressed proteins may serve as potential drug targets for CE.

To evaluate the diagnostic efficiency of four anti-cysticercus IgG, IgG4 or IgM antibody test kits (enzyme-linked immunosorbent assay, ELISA) by different manufacturers, so as to provide insights into the epidemiological investigation and clinical detection of cysticercosis.

Forty serum samples from cerebral cysticercosis patients, 100 serum samples from healthy volunteers, 30 serum samples from paragonimiasis skrjabini patients, 17 serum samples from cystic echinococcosis and 19 serum samples from subcutaneous or cerebral sparganosis patients were collected and detected using anti-cysticercus IgG, IgG4 or IgM antibody test kits (brand A) and the anti-cysticercus IgG antibody test kit (brand B). The sensitivity, specificity and false negative rate of the four kits for detection of cysticercosis were estimated.

The anti-cysticercus IgG, IgG4 or IgM antibody test kits (brand A) showed 95.00% (38/40), 87.50% (35/40), 7.50% (3/40) sensitivities and 98.00% (98/100), 100.00% (100/100) and 100.00% (100/100) fodiseases and stability.

To analyze the polymorphism of

lactate dehydrogenase (

) gene and predict B-cell epitopes in pLDH peptides in four species of human malaria parasites.

The blood samples and epidemiological characteristics were collected from malaria cases in Yunnan Province registered in the National Notifiable Disease Report System. The

genes of four human

species were amplified using nested PCR assay and sequenced. The polymorphisms of

genes was analyzed using the software MEGA version 7.0.26 and DnaSP version 5.10, and the B-cell epitopes were predicted in pLDH peptides using the Immune Epitope Database (IEDB).

The sequences of

LDH (

),

LDH (

),

LDH (

) and

LDH (

) genes were obtained from 153, 29, 17 and 11 blood samples from patients with

,

,

and

malaria, respectively, which included 15, 2, 4 and 2 haplotypes and had a nucleotide diversity (π) of 0.104. A high level of intra-species differentiation was seen in the

gene (π = 0.012), and the π values were all < 0.001 for

ile PvLDH, PfLDH and PmLDH genes may maintain a relatively conservative state. There may be two B-cell epitopes "212-EEVEGIFDR-220" and "208-LISDAE-213" in the proximal region of the C terminal in the pLDH peptide chain, which is feasible to differentiate between P. vivax and P. falciparum infections.

To understand the real prevalence of

infections in the freshwater fish in mainland China, so as to provide insights into clonorchiasis control and detection of freshwater fish.

All literatures reporting the prevalence of

infections in the freshwater fish, the second intermediate host of the parasite, were jointly retrieved in Chinese and English electronic databases from January 1, 2010 to December 31, 2020, including Wanfang Data, CNKI, PubMed, Web of Science, Embase and Cochrane Library. All studies were screened based on inclusion and exclusion criteria, and the quality of all enrolled literatures was evaluated. The pooled prevalence of

infections in freshwater fish and its 95% confidence interval (

) were estimated using the software Stata version 15.0, and subgroup analyses were performed to investigate the region-, season- and sample source-specific pooled prevalence of

infections in freshwater fish. In addition, the sensitivity and publication bias of all included studies were analyzed.2%, 95%

(0.17, 0.33)] than from retail trades [22.2%, 95%

(0.17, 0.28)] and breeding chain [12.3%, 95%

(0.03, 0.22)]. However, all included studies had a publication bias with a low sensitivity.

The prevalence of

infections is high in freshwater fish in mainland China, and there are still challenges for clonorchiasis control. Reinforcement of health education, diagnostics development and food safety supervision is recommended in future clonorchiasis control programs.

The prevalence of C. sinensis infections is high in freshwater fish in mainland China, and there are still challenges for clonorchiasis control. find protocol Reinforcement of health education, diagnostics development and food safety supervision is recommended in future clonorchiasis control programs.