Skafteabdi9932
They had higher levels of plasma concentration CT-1. The threshold levels of CT-1 for screening diagnosis of essential hypertension with hypertrophy and chronic heart failure in males (who were residents of the Podillya region of Ukraine) were evaluated.
Conclusions In patients with essential hypertension of varying severity, the GA+АА genotypes of CT-1 gene was found to be dominant. They had higher levels of plasma concentration CT-1. The threshold levels of CT-1 for screening diagnosis of essential hypertension with hypertrophy and chronic heart failure in males (who were residents of the Podillya region of Ukraine) were evaluated.
The aim is to establish a unified version of the biological method for babesiosis detection in vivo.
Materials and methods samples (n=257) of biological material of different origin were examined. These included blood samples from patients (n=6) and cattle (n=15); salivary gland homogenates (n=28) from 147 imagoes of ticks of the family Ixodidae, 32 imagoes of Ixodes ricinus and 115 imagoes of Dermacentor reticulates; spleen homogenates (n=63) from mouse-like rodents (Muridae) of the genera Myodes, Microtus, Apodemus and Sylvaemus. In order to cultivate in vivo Babesiae of the species B. microti, Syrian hamsters were infected with spleen homogenates from mouse-like rodents; for cultivating the B. divergens species Mongolian gerbils and nonlinear white mices were infected with blood samples from patients and cattle and salivary gland homogenates from ixodic ticks. The technology of modeling was based on the group specificity (differences in susceptibility to parasites and in parameters of morbidity) of thervoir (Syrian hamsters, Mongolian gerbils and nonlinear white mices with an increased level of susceptibility to parasites) followed by the immunocompromise formation. The use of the improved version of biological method increased the total rate of revealing of Babesiae, therewith creating an objective basis for optimizing the available ways of detection and study of Babesiae in vivo.
The aim The aim of our research is to study the features of toxic osteomyelitis in drug addicts, their diagnosis and comprehensive treatment, aimed at strengthening motivation for the suspension of the use of psychoactive substances and the elimination of the pathological process in the lower jaw.
Materials and methods The features of toxic osteomyelitis, complicated by abuse, have been studied on 46 patients in the department of oral and maxillofacial surgery of Lviv regional clinical hospital during 2013-2019. Psychoactive substances, used by the patients, varied from homemade drugs "Screw" taken by 32 men (69.5%), synthetic drug amphetamine consumed by 10 men (21,7%) to Subutex used by 4 patients (8,7%) for their pleasure. All the patients underwent comprehensive examination, which included clinical, laboratory, radiological, pathohistological studies. Almost all patients - 41 ( 89.1% ) underwent sequestrectomy.
Results Clinical picture progressed quite rapidly in the form of diffuse destructive-necrotic osteomyelitis of the mandible, which was characterized by a severe, atypical course of the pathological process with permanent inclusion of other additional areas of the lesion of the mandible. The X-ray at this stage showed an increase in destructive processes in the bone.
Conclusions Appropriate surgical tactics and pathogenetic therapy are of great importance for toxic osteomyelitis in drug addicts. Due to the treatment, despite the total destruction of the mandible, it was possible to stop the destructive bone processes and to preserve life for such patients.
Conclusions Appropriate surgical tactics and pathogenetic therapy are of great importance for toxic osteomyelitis in drug addicts. Due to the treatment, despite the total destruction of the mandible, it was possible to stop the destructive bone processes and to preserve life for such patients.
The aim Our study aimed at evaluating the relationships between sleep disorders (SD), cognitive impairment (CI), anxiety and depression in patients with relapsing-remitting multiple sclerosis (RRMS).
Materials and methods One hundred and five patients with RRMS (80 females and 25 males) aged from 22 to 67 years (mean age 41,8±10,7; EDSS3,5±1,6; disease duration (DD) 10,3±8,5 years) were enrolled into the study. All participants completed questionnaires on sleep (the Pittsburgh Sleep Quality Index /PSQI), cognitive functions (The Montreal Cognitive Assessment /MoCA), anxiety (Hamilton Anxiety Rating Scale /HAM-A), depression (Beck Depression Inventory/ BDI).
Results According to PSQI score the patients were divided into two groups with (n=42) and without SD (n=63). The patients with SD were older (45,36±1,66 vs 39,41±1,27, p=0.005), had higher EDSS score (3,98±0,26 vs 3,14±0,19, p=0,008), BDI (13,79±1,14 vs 8,96±0,86, p=0,0009) and HAM-A (24,52±1,42 vs 16,56±0,99, p<0,0001) scales compared with patients without SD. The frequency of anxiety (p=0,0034) and depression (p=0,038) was significantly higher in RRMS patients with compared to those without SD. No significant difference was found in gender, DD and MoCA score. In patients with SD significant negative correlation between MoCA and BDI score (r = -0,42, p<0,005) was found. In the group of patients without SD significant negative correlation between MoCA and EDSS (r = -0,27, p=0,03), MoCA and BDI (r = -0,26, p=0,043),) MoCA and HAM-A (r = -0,25, p=0,041) score was detected.
Conclusions Insomnia type SD in RRMS patients were associated with older age, higher EDSS score and presence of anxiety and depression.
Conclusions Insomnia type SD in RRMS patients were associated with older age, higher EDSS score and presence of anxiety and depression.
The aim To study the influence of chemical, physical factors on the biofilm forming activity of P. aeruginosa, A. baumannii.
Materials and methods Biofilm forming activity of P. aeruginosa (10 isolates) and A. baumannii (10 isolates) was studied in nutrient media of different composition. There was used the method in 96-well crystalline violet staining plates with spectrophotometry (STAT FAX®4300, wavelength of 620 nm).
Results Results showed that in standard medium (trypto-soy broth), strains of P. aeruginosa (90%) and A. baumannii (60%) obtained high biofilm forming activity. A. baumannii formed biofilms even in sterile water. Biofilm forming activity of urease positive P. aeruginosa increased in the medium with 1.0% urea. Both Acinetbacteria and Pseudomonas intensively produced their biofilms in the presence of 5% serum or sub-bacteriostatic concentrations of levofloxacin in the media. High concentrations of sodium chloride inhibited their biofilm activity.
Conclusions Isolates of Acinetobacter and Pseudomonas obtain the protective biofilm-forming ability under such adverse environmental conditions as insufficient nutrients, high osmotic pressure, the presence of antibiotics but at high concentrations sodium chloride biofilm-formation is stimulated only in the first bacteria and suppressed in the second one.
Conclusions Isolates of Acinetobacter and Pseudomonas obtain the protective biofilm-forming ability under such adverse environmental conditions as insufficient nutrients, high osmotic pressure, the presence of antibiotics but at high concentrations sodium chloride biofilm-formation is stimulated only in the first bacteria and suppressed in the second one.
The aim Is to study the morphological features of rats' hearts after prenatal administration of glucocorticoids.
Materials and methods In this study we used histological, immunohistochemical, electron-microscopic and statistical research methods.
Results It is found that at 30th day after birth in rats after intrafetal introduction of dexamethasone in myocardium a relative area occupied by arterial vessels is significantly smaller in comparison with control. KU-0063794 supplier Absolute and relative number of Ki-67+-cardiomyocytes in the myocardium of experimental rats is reduced throughout the second week after birth and is significantly less compared to the control group. In the nuclei of cardiomyocytes of experimental rats is rendered the greater amount of heterochromatin in comparison with cardiomyocytes of the control group where euchromatin prevails.
Conclusions After intrafetal injection of dexametazone changes in dynamics and significantly smaller index of relative area occupied by arterial vessels in ventricular myocardium at the 30th day after birth are observed; the absolute and relative number of Ki-67+ -cardiomyocytes in myocardium decreases during the second week after birth and is significantly lower compared to the control group; in the nuclei of cardiomyocytes of experimental rats a greater amount of heterochromatin is visualized, and in cardiomyocytes of the control group - euchromatin.
Conclusions After intrafetal injection of dexametazone changes in dynamics and significantly smaller index of relative area occupied by arterial vessels in ventricular myocardium at the 30th day after birth are observed; the absolute and relative number of Ki-67+ -cardiomyocytes in myocardium decreases during the second week after birth and is significantly lower compared to the control group; in the nuclei of cardiomyocytes of experimental rats a greater amount of heterochromatin is visualized, and in cardiomyocytes of the control group - euchromatin.
The aim To determine the set of structural and functional changes in pancreatic islets (PI) of obesity-induced insulin resistant (IR) rats of different age (young and old) fed with prolonged (6 month) high-fat diet (HFD) (58% of fat) and further treatment with N-Stearoylethanolamine (NSE), a bioactive N-Acylethanolamine.
Materials and methods Alimentary obesity-induced IR model in rats of two age groups was used to investigate the influence of age and NSE treatment on pancreas morphology (using histological, histochemical and immunohistochemical techniques) and on several biochemical parameters associated with DM onset.
Results The NSE administration normalized pancreas morphology which was more affected in the old IR group; the signs of inflammation, edema, fibrosis and steatosis were somehow diminished and PI area became significantly increased. The amount of the A-F-positive insulocytes increased and TUNEL-positive - decreased. Compensatory hyperplasia in the affected pancreas of both age was an important indicator of NSE stimulating effect.
Conclusions Protective effects of NSE on morpho-functional state of pancreas in HFD-induced IR rats of both age are associated not only with its anti-inflammatory, anti-oxidant and anti-dyslipidemic properties but also with activation of PI hyperplasia and β-cells compensatory mechanisms.
Conclusions Protective effects of NSE on morpho-functional state of pancreas in HFD-induced IR rats of both age are associated not only with its anti-inflammatory, anti-oxidant and anti-dyslipidemic properties but also with activation of PI hyperplasia and β-cells compensatory mechanisms.
The aim To determine the degree of correlation of mass of the fetus and the level of mir-21, mir210 in maternal blood and umbilical cord blood of the fetus in uncomplicated gestation.
Materials and methods 60 pregnant women with a single baby pregnancy in the third trimester (37-40 weeks) were examined. They all were given a general clinical, obstetric and the level of miRNA21-3р and miRNA210-3р were determined in the whole blood of pregnant women (before labor) and in fetal blood obtained from the umbilical artery at birth. The level of miRNAs was determined by the TaqMan method.
Results After examining maternal and fetal plasma samples, we were able to determine 49 samples of hsa-miR210-3p and hsa-miR21-3p from maternal plasma, 44 samples of hsa-miR210-3p and 37 samples of hsa-miR21-3p from the cord blood, which is a satisfactory result of more than 50%. Subsequently, between the results obtained and the birth weight of the fetus Pearson's correlation coefficient was studied. According to the results obtained, we found no correlation between fetal mass and hsa-miR210-3p level in maternal plasma (r-0,068674), low positive correlation of fetal mass with hsa-miR21-3p level in maternal plasma (r-0,212181 ), an average positive correlation with the level of hsa-miR21-3p in umbilical cord blood (r- 0.
