Lumcnulty7627

5-fold decrease), which was attributed to a more connected network structure due to the higher aspect ratio of the nanofibers. Finally, both nanoparticle types produced an increase in the activation energy of thermal degradation.Although remarkable progress has been made toward understanding the structures of eukaryotic RNA polymerases, the pathways and factors that facilitate their assembly remain unresolved. Essential proteins Rba50 and Gpn2 are required for Rpb3 subcomplex assembly, but whether they participate in subsequent assembly steps is unknown. Herein, we performed comprehensive genetic screens to explore Rba50 function. We identified two unique extragenic rba50-3-suppressing mutations that map to genes encoding the Rba50-interacting protein Gpn2, and Rpb2, the second largest subunit of RNAPII. Both gpn2-R347S and rpb2-V1171G variants bypass Rpb1 cytoplasmic arrest and temperature-sensitive growth defects of the rba50-3 mutant. GPN2 and RPB2 were also identified as novel multicopy suppressors of the rba50-3 mutant. Rapid depletion of Rba50 affected Rpb3-Rpb2 association during RNAPII assembly. Importantly, we demonstrated that Gpn2 facilitates the association of Rba50 and Rpb2. Our results imply that Rba50-Gpn2 interaction is essential for Rpb2 recruitment during RNAPII assembly following Rpb3 subcomplex assembly. Furthermore, the Rba50-Gpn2 complex appears to play a similar role in the assembly of RNAPIII. We therefore propose a model in which Rba50 interacts with Gpn2 and thereby promotes loading of the second largest subunit of RNAP II and III onto the previously assembled subcomplex.Gardenia jasminoides Ellis is a well-known herbal medicine. In this study, the effect of G. jasminoides Ellis polysaccharide (GPS) on liver injury in an alpha-naphthylisothiocyanate (ANIT)-induced cholestatic mouse model and the associated molecular mechanisms were investigated. GPS administration dose-dependently ameliorated impaired hepatic function, including a 2-7-fold decrease in aminotransferase levels, ameliorating tissue damage, upregulating the expression of farnesoid X receptor (FXR) and pregnane X receptor (PXR) and their downstream efflux transporters, and decreasing the levels of 12 bile acids (BAs), in cholestatic mice. Furthermore, GPS ameliorated gut microbiota dysbiosis, improved intestinal barrier function, and reduced serum and hepatic lipopolysaccharide levels 1.5-fold. GPS also inhibited the Toll-like receptor 4 (TLR4)/nuclear factor kappa-B (NF-κB) signaling, decreased the expression of inflammatory factor genes, and ameliorated hepatic inflammation. Notably, fecal microbiota transplantation from GPS-fed mice also increased the hepatic expression of FXR, PXR, and efflux transporters; decreased the levels of 12 BAs; restored intestinal barrier function; and decreased hepatic inflammation mediated by the TLR4/NF-κB pathway. In conclusion, GPS has a protective effect against cholestatic liver injury through modulation of gut microbiota and inhibition of the TLR4/NF-κB pathway. Regulating gut microbiota using herbal medicine polysaccharides may hold unique therapeutic promise for cholestatic liver diseases.In our clinical work with treatment-resistant depressed (TRD) patients, certain patterns have emerged in terms of patients' presentations of anhedonia. Multiple studies have investigated anhedonia either as a state or a trait variable in depression, but anhedonia is a multifaceted construct and, as we better understand anhedonia, we increase our ability to measure and treat it. With the aim of personalizing medicine for more efficacious treatments, this paper focuses on clinical patterns we have seen in anhedonic TRD and argues for studies to be done in support of translational, face, and construct validity. Three representative clinical presentations of TRD with anhedonia are described a congenital type, a stress-induced type, and a type exacerbated by medication. Each case is followed with discussion relating the clinical features to clinical and preclinical research relevant to putative mechanisms for the case. Animal models that are best suited to investigate and validate the existence of etiologies and mechanisms underlying anhedonia constructs unique to each case are proposed, as are potential directions for research in humans.Behavioural phase change initiates and functionally couples the suite of traits that comprise density-dependent polyphenism in locusts. Here I provide a semi-expurgated account of my 25-year research journey studying behavioural phase transition in the desert locust. The journey spans continents, involves a cast of extraordinary colleagues, and travels across levels of biological organisation from deep within the nervous system of individual locusts to mass migration and the evolution and population dynamics of swarming.Heavy metals in watersheds are a serious concern due to their toxicity, abundance, and persistence in the environment, especially in mining areas. Source analyses and exploration of other related factors are one of the most important methods to help with effective prevention and control of heavy metal pollution in watersheds. In this study, the concentrations of Cr, Co, Ni, Cu, Zn, As, Cd, Sb, Ba and Pb were measured in the Le'an River, and PCA (principal component analysis) and APCS-MLR (absolute principal component scores - multivariate linear regression) methods were used to identify the sources of the dissolved heavy metals. Additionally, a CA (correlation analysis) method was used to explore the correlations between landscape indices and concentrations of heavy metals. Results show that the main sources for these dissolved heavy metals are mining activities, fertilizers, pesticides, and natural sources. Specific results of PCA and APCS-MLR suggest that Cu, Zn, Cd, Ba are mainly related to mining activities, Cr and Pb are due to fertilizers and pesticides, and Co and Ni are mainly due to natural sources. Correlations between landscapes and heavy metals revealed significant temporal variations, with the strongest responses of dissolved heavy metals to landscape indices appearing in December and March. The propensity of positive or negative responses of the heavy metals to landscape indices are determined by the sources, and their temporal variations may be related to the seasonal changes of rainfall and plant metabolism.The associations between particulate matter (PM) exposure, psychosocial stress and blood cell parameters are bringing novel insights to characterize the early damage of multiple diseases. Based on two studies conducted in three Chinses cities using cross-sectional (Beijing, 425 participants) and panel study (Tianjin and Shanghai, 92 participants with 361 repeated measurements) designs, this study explored the associations between short-term exposure to ambient PM and blood cell parameters, and the effect modification by psychosocial stress. Increasing PM2.5 exposure was significantly associated with decreases in red blood cell (RBC) count and mean corpuscular hemoglobin concentration (MCHC), and increases in mean corpuscular volume (MCV), platelets count (PLT) and platelet hematocrit (PCT) in both studies. For instance, a 10 μg/m3 increment in PM2.5 concentration was associated with a 1.04% (95%CI 0.16%, 1.92%) increase in PLT (4-d) and a 1.09% (95%CI 0.31%, 1.87%) increase in PCT (4-d) in the cross-sectional study, and a 0.64% (95%CI 0.06%, 1.22%) increase in PLT (1-d) and a 0.72% (95%CI 0.33%, 1.11%) increase in PCT (1-d) in the panel study, respectively. In addition, stronger increases in MCV, PLT, and PCT associated with PM2.5 exposure were found in higher psychosocial stress group compared to lower psychosocial stress group (p for interaction less then 0.10), indicating that blood cell parameters of individuals with higher psychosocial stress might be more susceptible to the early damages of PM2.5 exposure.As the most important contributors to global warming in recent decades, anthropogenic carbon dioxide (CO2) and black carbon (BC) play significant roles in driving the global/regional hydrological cycle. Most of previous studies on the climate effects of CO2 and BC focused on tropics and monsoon regions. The influences and their differences of CO2 and BC on the precipitation in Northern Hemisphere mid-latitudes (NHML) have not been paid enough attention. Here we investigate the NHML precipitation responses to a tenfold increase in BC and a doubling of CO2 by analyzing the multi-model simulation results from the Precipitation Driver Response Model Intercomparison Project (PDRMIP). Our results show that the NHML precipitation changes induced by BC and CO2 distinctly differ in trends and seasons. The increased BC will reduce the NHML precipitation, especially in summer, whereas the doubled CO2 will enhance the regional precipitation, mainly in winter. The differences between the BC and CO2 induced NHML precipitation changes are most distinct in Central Asia and central North America. Further analyses reveal the underlying mechanisms of the distinct responses of precipitation the decrease in NHML precipitation induced by BC aerosols mainly results from the dynamic effect by reducing the temperature gradient, thereby weakening the zonal wind, while the increased precipitation by CO2 is caused by the increase in atmospheric water vapor through the thermodynamic effect. The results of these simulations are helpful for understanding the mechanism of anthropogenic precipitation changes in mid-latitudes.This study aims to investigate the adsorption characteristics of cationic surfactant, cetyltrimethylamonium bromide (CTAB) onto negatively nanosilica rice husk surface and the application for antibiotic treatment in water environment. Adsorption of CTAB onto nanosilica increased with an increase of solution pH, due to an enhancement of the electrostatic attraction between cationic methylamomethylamonium groups and negatively charged nanosilica surface enhanced at higher pH. Adsorption of CTAB decreased with a decrease of ionic strength while a common intersection point (CIP) was observed for adsorption isotherm at different ionic strengths, suggesting that hydrophobic interactions between alkyl chains in CTAB molecules significantly induced adsorption and admicelles with bilayer formation were dominant than monolayer of hemimicelles. The CTAB functionalized nanosilica (CFNS) was applied for removal of beta-lactam amoxicillin (AMX). The best conditions for AMX treatment using CFNS were selected as pH 10, contact time 60 min and CFNS dosage 10 mg/mL. Removal efficiency of AMX using CFNS reached to 100% under optimum conditions while it was only 25.01% using nanosilica without CTAB. CDK inhibitor drugs The maximum AMX adsorption capacity using CFNS of about 25 mg/g was much higher than other adsorbents. The effects of different organics such as humic acid, anionic surfactant, and other antibiotics on AMX removal using CFNS were also studied. A two-step model can fit CTAB uptake isotherms onto nanosilica and AMX onto CFNS well at different KCl concentrations. Based on the desorption of CTAB with AMX adsorption as well as adsorption isotherms, the change in surface charge and functional vibration groups after adsorption, we indicate that AMX adsorption onto CFNS was mainly controlled by electrostatic interaction. We reveal that CFNS is an excellent adsorbent for antibiotic treatment from aqueous solution.