Mcgregoratkinson2215

especially under glucose deficiency.

Our study demonstrated that the AB aqueous enema alleviated colitis by restoring intestinal barrier proteins and regulating the gut microbiota.

Our study demonstrated that the AB aqueous enema alleviated colitis by restoring intestinal barrier proteins and regulating the gut microbiota.Cancer is one of the major causes of human death per year. In recent years, cancer identification and classification using machine learning have gained momentum due to the availability of high throughput sequencing data. Using RNA-seq, cancer research is blooming day by day and new insights of cancer and related treatments are coming into light. In this paper, we propose PanClassif, a method that requires a very few and effective genes to detect cancer from RNA-seq data and is able to provide performance gain in several wide range machine learning classifiers. We have taken 22 types of cancer samples from The Cancer Genome Atlas (TCGA) having 8287 cancer samples and 680 normal samples. Firstly, PanClassif uses k-Nearest Neighbour (k-NN) smoothing to smooth the samples to handle noise in the data. Then effective genes are selected by Anova based test. For balancing the train data, PanClassif applies an oversampling method, SMOTE. We have performed comprehensive experiments on the datasets using several classification algorithms. Experimental results shows that PanClassif outperform existing state-of-the-art methods available and shows consistent performance for two single cell RNA-seq datasets taken from Gene Expression Omnibus (GEO). PanClassif improves performances of a wide variety of classifiers for both binary cancer prediction and multi-class cancer classification. PanClassif is available as a python package (https//pypi.org/project/panclassif/). All the source code and materials of PanClassif are available at https//github.com/Zwei-inc/panclassif.The eukaryotic transcriptome undergoes various post-transcriptional modifications which assists gene expression. Polyadenylation is a molecular process occurring at the 3'-end of the RNA molecule which involves the poly(A) polymerase attaching adenine monophosphate molecules in a chain-like fashion to assemble a poly(A) tail. Multiple RNA isoforms are produced with differing 3'-UTR and exonic compositions through alternative polyadenylation (APA) which enhances the diversification of alternatively spliced mRNA transcripts. To study polyadenylation patterns, novel methods have been developed using short-read and long-read sequencing technologies to analyse the 3'-ends of the transcript. click here Recent studies have identified unique polyadenylation patterns in different cellular functions, including oncogenic activity, which could prove valuable in the understanding of medical genetics, particularly in the discovery of biomarkers in diseased states. We present a review of current literature reporting on polyadenylation and the biological relevance in the mammalian transcriptome, with a focus on the human transcriptome. Additionally, we have explored the various methods available to detect polyadenylation patterns using second and third generation sequencing technologies.Fertilization is a central event during the life cycle of most eukaryotic organisms and involves gamete recognition and fusion, ultimately resulting in zygote formation. Gamete fertilization in the malaria-causing Plasmodium parasites occurs inside the mosquito midgut and represents a major bottleneck in the life cycle. Cysteine Rich Secretory Proteins (CRISPs) are key molecules involved in fertilization in vertebrates and the presence of a CRISP ortholog in human malaria infective Plasmodium falciparum suggested a possible role in fertilization. Strikingly, P. falciparum CRISP exhibited a unique terminal localization in the male microgamete. Parasites with a CRISP gene deletion (P. falciparum crisp-) proliferated asexually similar to wildtype NF54 parasites and differentiated into gametocytes. Further analysis showed that Plasmodium falciparum crisp- gametocytes underwent exflagellation to form male gametes and no apparent defect in transmission to the mosquito vector was observed. These data show that P. falciparum CRISP is a marker for the apical end of the microgamete and that it might only have an ancillary or redundant function in the male sexual stages.The object of this work was to synthesize an iron and aminoacetic acid sequentially modified hierarchical porous biochar (AC-Fe@HPBC) for tetracycline (TC) removal from aqueous solution. Results showed that AC-Fe@HPBC had a larger surface area (362.5370 m2/g), developed microporous structure (0.1802 cm3/g), and numerous functional groups, which provided more adsorption sites. The maximum adsorption capacity towards TC by AC-Fe@HPBC was 457.85 mg/g, 1.43, 1.29 and 1.20-fold than that of HPBC, AC@PHBC and Fe@HPBC, respectively, and the super-fast adsorptive equilibrium was achieved within 10 min. Additionally, introducing amino and carboxyl functional groups on the AC-Fe@HPBC surface significantly broadened the operation pH range (3-11). Site energy analysis indicated TC and AC-Fe@HPBC had stronger adsorption affinity at a higher temperature. The adsorption mechanism involved pore filling, surface complexation, H-bond and π-π interaction. Moreover, the reusability experiments proved AC-Fe@HPBC as an effective adsorbent for TC removal from aqueous solution.Microalgae technology is a promising method for treating piggery digestate, while its removal ability of humic acids (HAs) is poor. Here, an electric field-microalgae system (EFMS) was used to improve the removal of HAs from the piggery digestate. Results indicated that the removal of HAs by EFMS relied on the initial concentration of HAs, electrical intensity, the initial inoculation concentration of microalgae and pH. Values of these parameters were optimized as electrical intensity of 1.2 V/cm, microalgae initial inoculation concentration of 0.1 g/L and pH 5.0. The HAs removal efficiency by EFMS (55.38%) was 13% and 38% higher than that by single electric field and microalgal technology. It was observed that oxidation, coagulation and assimilation contributed to the removal of HAs, suggesting that EFMS could serve as an attractive and cost-effective technique for the removal of HAs from the piggery digestate.Nanoprecipitation is a practical method to prepare carriers at the nanometric scale, which attracts attention in pharmaceutics because of its low cost, easy setup, the versatility of the starting materials, possibility to obtain different kinds of carriers, and minimal environmental impact. Since 1986, this technique has been extensively employed in research; therefore, this paper focuses on state of art regarding inventions wherein it is employed. To this end, 133 nanoprecipitation-based patent families are identified in the PatSnap® platform, which allows identifying general trends. Afterwards, a sample of 40 patent families reported as granted (21 families) or patent applications (19 families) during the last decade are studied in depth to establish the research tendencies. Undoubtedly, Chinese universities are positioned as leaders in this field, and cancer treatments are the more claimed use followed far behind for developments targeting neurodegenerative and diabetes diseases. New proposals on targeted and stimuli response particles are also claimed, and development of polymers, prodrugs, and improvements to the technique such as the flash-nanoprecipitation, use of microfluidics, or design of green process are relevant. Interestingly, nanoprecipitation-related patent families have significantly increased during the last decade, being the 71% of the total, which makes alluring the perspectives about its industrial harnessing.The influence of amino acids, other than leucine, in improving aerosolization of inhalable powders has not been widely explored. This detailed study focused on the use of methionine, another promising endogenous amino acid, in high dose spray-dried co-amorphous powders by investigating the influence of methionine proportion (0 - 20% w/w), and feed concentration (0.2 - 0.8% w/v) on aerosolization of kanamycin, a model drug, using a design of experiment approach. Low frequency Raman spectroscopy was used to assess the stability of the powders stored at 25 °C/53% relative humidity over 28 days. An increase in concentration of methionine was associated with an increase in fine particle fraction (FPF), with the highest FPF of 84% being achieved at 20% w/w and 0.2% w/v feed concentration. With an increase in feed concentration, both yield and particle size increased for all formulations; the FPF did not change except for kanamycin only formulation in which it decreased. During storage at high humidity, similar aerosolization stabilities were offered by different proportions of methionine although methionine crystallized out in all formulations. Furthermore, the crystallization was accompanied by surface enrichment of methionine on the particles. This study suggests that there is a direct relationship between methionine content and aerosolization for kanamycin-methionine amorphous matrices but feed concentration has little effect. In addition, methionine proportion has no effect on physical stability of such matrices at high humidity.For effective topical and transdermal drug delivery, it is necessary for most actives to penetrate and permeate through the stratum corneum (SC). Extensive investigation of the thermal behaviour of mammalian SC has been performed to understand the barrier function of the skin. However, little attention has been paid to the related experimental variables in thermal analysis of the SC using differential scanning calorimetry that may influence the results obtained from such studies. In this review, we provide a comprehensive overview of the thermal transitions of the SC of both porcine and human skin. More importantly, the selection and impact of the experimental and instrumental parameters used in thermal analysis of the SC are critically evaluated. New opportunities for the use of thermal analysis of mammalian SC in advancing skin research, particularly for elucidation of the actions of excipients employed in topical and transdermal formulations on the skin are also highlighted.Dry powder inhalers (DPI) are well established products for the delivery of actives via the pulmonary route. Various DPI products are marketed or developed for the treatment of local lung diseases such as chronic obstructive pulmonary disease (COPD), asthma or cystic fibrosis as well as systemic diseases targeted through inhaled delivery (i.e. Diabetes Mellitus). One of the key prerequisites of DPI formulations is that the aerodynamic size of the drug particles needs to be below 5 µm to enter deeply into the respiratory tract. These inherently cohesive inhalable size particles are either formulated as adhesive mixture with coarse carrier particles like lactose called carrier-based DPI or are formulated as free-flowing carrier-free particles (e.g. soft agglomerates, large hollow particles). In either case, it is common practice that drug and/or excipient particles of DPI formulations are obtained by processing API and API/excipients. The DPI manufacturing process heavily involves several particle and powder technologies such as micronization of the API, dry blending, powder filling and other particle engineering processes such as spray drying, crystallization etc.