Lucasroman1879

Insect chemoreception, including olfaction and gustation, involves several families of genes, including odorant receptors (ORs), ionotropic receptors (IRs), and gustatory receptors (GRs). The variegated cutworm Peridroma saucia Hübner (Lepidoptera Noctuidae) is a worldwide agricultural pest that causes serious damage to many crops. To identify such olfactory and gustatory receptors in P. saucia, we performed a systematic analysis of the antennal transcriptome of adult P. saucia through Illumina sequencing. A total of 103 candidate chemosensory receptor genes were identified, including 63 putative ORs, 10 GRs, 24 IRs, and 6 ionotropic glutamate receptors (iGluRs). Phylogenetic relationships of these genes with those from other species were predicted, and specific chemosensory receptor genes were analyzed, including ORco, pheromone receptors (PRs), sugar receptors, CO2 receptors, and IR co-receptors. RT-qPCR analyses of these annotated genes revealed that 6 PRs were predominantly expressed in male antennae; 3 ORs, 1 GR, 2 IRs, and 2 iGluRs had higher expression levels in male than in female antennae; and 14 ORs, 1 GR, and 3 IRs had higher expression levels in female than in male antennae. This research increases the understanding of olfactory and gustatory systems in the antennae of P. saucia and facilitates the discovery of novel strategies for controlling this pest. Copyright © 2020 Sun, Dong, Gu and Wang.Reptiles are critically affected by temperature throughout their lifespan, but especially so during early development. Temperature-induced changes in phenotype are a specific example of a broader phenomenon called phenotypic plasticity in which a single individual is able to develop different phenotypes when exposed to different environments. With climate change occurring at an unprecedented rate, it is important to study temperature effects on reptiles. For example, the potential impact of global warming is especially pronounced in species with temperature-dependent sex determination (TSD) because temperature has a direct effect on a key phenotypic (sex) and demographic (population sex ratios) trait. Reptiles with TSD also serve as models for studying temperature effects on the development of other traits that display continuous variation. Temperature directly influences metabolic and developmental rate of embryos and can have permanent effects on phenotype that last beyond the embryonic period. For instance temperature sensors and assessment of genetic variation for thermosensitivity. Copyright © 2020 Singh, Das and Rhen.The maturation of rhythmic jaw movements (RJMs) and related neuromuscular control has rarely been studied in animals, though this process is essential for regulating the development of stomatognathic functions. Previous studies have shown that occlusal hypofunction during growth alters masticatory performance. However, little is known about patterns of cortically-induced RJMs under conditions of soft-diet feeding during development. The aim of this study is to clarify the effect of low occlusal loading on the pattern of cortically induced RJMs and related neuromuscular responses in growing rats. Sixty-four 2-week-old male albino Wistar rats were randomly divided into two groups and fed on either a normal diet (control) or soft diet (experimental) soon after weaning. At 5, 7, 9, and 11 weeks of age, electromyographic (EMG) activity was recorded from the right masseter and anterior digastric muscles along with corresponding kinematic images in RJMs during repetitive intracortical microstimulation of the left coe neuromuscular response from specific stimulation of the CMA. Copyright © 2020 Aung, Kato, Abe, Ogawa, Ishidori, Fujita, Okihara, Kokai and Ono.Purpose Endurance runners training with high-intensity intermittent exercise might experience damage to cardiac muscle. We have therefore compared changes of cardiac biomarkers after workload-matched intermittent and continuous exercise. Methods Twelve endurance runners [11 males, 1 female; means ± SD V . O2 max, 62.4 ± 5.4 ml kg-1 min-1; velocity of V . O2 max (v V . O2 max), 17.1 ± 1.4 km h-1] completed an intermittent and continuous exercise trial in random order. Intermittent exercise consisted of running at 90% v V . O2 max for 2 min followed by 50% v V . O2 max for 2 min, repeated for 92 min. Continuous exercise was performed at 70% v V . find more O2 max for 92 min. Blood samples were drawn before and 0, 2, 4, 24, and 48 h after exercise for assay of various cardiac biomarkers. Changes in concentration of biomarkers were averaged for the comparison of intermittent with continuous exercise after adjustment for baseline concentration and exercise intensity expressed as percent of heart-rate reserve (%HRR); magnitudes were assessed by standardization. Results There were moderate and large increases in high-sensitivity cardiac troponin-I and -T respectively following exercise. The differences between the increases adjusted to the mean intensity of 78 %HRR were trivial, but at 85 %HRR the increases for cardiac troponin-I and -T were moderately higher for intermittent compared with continuous exercise (factor difference, ×/÷90% confidence limits 3.4, ×/÷1.9 and 2.1, ×/÷1.8 respectively). Differences in the changes in other cardiac biomarkers were trivial. Conclusion Prolonged intermittent exercise is potentially more damaging to cardiac muscle than continuous exercise of the same average running speed at higher average heart rates in endurance runners. Copyright © 2020 Li, Nie, Zhang, Fu, Yi, Hopkins, Liu and Lu.Increasing water CO2, aquatic hypercapnia, leads to higher physiological pCO2 levels in fish, resulting in an acidosis and compensatory acid-base regulatory response. Senegalese sole is currently farmed in super-intensive recirculating water systems where significant accumulation of CO2 in the water may occur. Moreover, anthropogenic releases of CO2 into the atmosphere are linked to ocean acidification. The present study was designed to assess the effects of acute (4 and 24 h) and prolonged exposure (4 weeks) to CO2 driven acidification (i.e., pH 7.9, 7.6, and 7.3) from normocapnic seawater (pH 8.1) on the innate immune status, gill acid-base ion transporter expression and metabolic rate of juvenile Senegalese sole. The acute exposure to severe hypercapnia clearly affected gill physiology as observed by an increase of NHE3b positive ionocytes and a decrease of cell shape factor. Nonetheless only small physiological adjustments were observed at the systemic level with (1) a modulation of both plasma and skin humoral parameters and (2) an increased expression of HIF-1 expression pointing to an adjustment to the acidic environment even after a short period (i.