Gutierrezbeier8260

The limitations to production of fatty alcohols by microbial cell factories are detailed along with consideration to potential research directions that may aid in achieving viable commercial scale production of fatty alcohols from renewable feedstock.Antimicrobial resistance remains a great threat to global health. In response to the World Health Organizations' global call for action, nature has been explored for novel and safe antimicrobial candidates. To date, fish have gained recognition as potential source of safe, broad spectrum and effective antimicrobial therapeutics. The use of computational methods to design antimicrobial candidates of industrial application has however, been lagging behind. To fill the gap and contribute to the current fish-derived antimicrobial peptide repertoire, this study used Support Vector Machines algorithm to fish out fish-antimicrobial peptide-motif candidates encrypted in 127 peptides submitted at the Antimicrobial Peptide Database (APD3), steered by their physico-chemical characteristics (i.e., positive net charge, hydrophobicity, stability, molecular weight and sequence length). The best two novel antimicrobial peptide-motifs (A15_B, A15_E) with the lowest instability index (-28.25, -22.49, respectively) and highest .094, -270.751; coefficient weight (CW) -763.6, 763.3 for A15_B and A15_E) whereas, Carbapenem synthetase (PDB 1q15) had a DES of -236.802, -262.75 and a CW of -819.7, -829.7 for peptides A15_B and A15_E, respectively. Motif A15_B of amino acid positions 2-19 in Pleurocidin exhibited the strongest in silico antimicrobial potentials. GSK'963 nmr This segment could be a good biological candidate of great application in pharmaceutical industries as an antimicrobial drug candidate.Aerogels have gained significant interest in recent decades because of their unique properties such as high porosity, low density, high surface area, and excellent heat and noise insulation. However, their high cost and low mechanical strength limit their practical application. We developed appropriate conditions to produce aerogels with controlled density, high mechanical strength, and thermal characteristics from bacterial cellulose (BC) synthesized by the strain Komagataeibacter sucrofermentans H-110. Aerogels produced using TEMPO oxidized BC (OBC) exhibited high mechanical strength and lower shrinkage than those from native bacterial cellulose (NBC). Compared to the NBC, the use of TEMPO-oxidized BC with oxidation degrees (OD) of 1.44 and 3.04% led to the reduction of shrinkage of the aerogels from 41.02 to 17.08%. The strength of the aerogel produced from the TEMPO-oxidized BC with an oxidation degree of 1.44% was twice that of the aerogel produced from NBC. The addition of Mg2+ at concentrations of 20 and 40 mM during the preparation of the aerogels increased the strength of the aerogels by 4.9 times. The combined use of TEMPO-oxidized BC and Mg2+ allowed pore size reduction from 1,375 to 197.4 μm on the outer part of the aerogels, thereby decreasing the thermal conductivity coefficient from 0.036 to 0.0176 W/(m•K). Furthermore, novel biocomposites prepared from the aerogels based on NBC and OBC and sodium fusidate, which have high antibiotic activity against Staphylococcus aureus, were obtained. Owing to their antibacterial properties, these aerogels can be used as functional biomaterials in a wide range of applications such as in tissue engineering and fabrication of wound dressing materials.Cell-derived extracellular matrices (CD-ECMs) captured increasing attention since the first studies in the 1980s. The biological resemblance of CD-ECMs to their in vivo counterparts and natural complexity provide them with a prevailing bioactivity. CD-ECMs offer the opportunity to produce microenvironments with costumizable biological and biophysical properties in a controlled setting. As a result, CD-ECMs can improve cellular functions such as stemness or be employed as a platform to study cellular niches in health and disease. Either on their own or integrated with other materials, CD-ECMs can also be utilized as biomaterials to engineer tissues de novo or facilitate endogenous healing and regeneration. This review provides a brief overview over the methodologies used to facilitate CD-ECM deposition and manufacturing. It explores the versatile uses of CD-ECM in fundamental research and therapeutic approaches, while highlighting innovative strategies. Furthermore, current challenges are identified and it is accentuated that advancements in methodologies, as well as innovative interdisciplinary approaches are needed to take CD-ECM-based research to the next level.Oral biofilms play an essential role on peri-implant disease development. Synthetic hydroxyapatite nanoparticles (nHAP) are a bioinspired material that has structural and functional similarities to dental enamel apatite and may provide preventive properties against biofilm formation. This study aimed to investigate the effects of an experimental nHAP solution on biofilm formation on polished and non-polished titanium under oral conditions. Five volunteers carried maxillary splints with non-polished and polished titanium and followed a 48 h rinsing protocol with the proposed nHAP solution, and with chlorhexidine 0.2% (CHX) and water, as controls. Samples were analyzed by fluorescence microscopy (FM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). FM showed a significant reduction of biofilms on polished samples treated with nHAP (p = 0.0485) compared with water, without differences between nHAP and CHX (p > 0.9999). Analyzing biofilm viability, polished samples rinsed with nHAP showed significantly fewer dead bacteria than CHX (p = 0.0079), but there was no significant difference in viability between polished samples rinsed with water and nHAP (p = 0.9268). A significantly higher biofilm coverage was observed on the non-polished surfaces compared to the polished surfaces when nHAP was applied (p = 0.0317). This difference between polished and non-polished surfaces was not significant when water (p = 0.1587) or CHX (p = 0.3413) rinsing were applied. SEM and TEM analysis supported the FM findings, that polished samples rinsed with nHAP presented fewer biofilm coverage compared to samples rinsed with water. In conclusion, the nHAP solution reduced the biofilm formation on polished Ti surfaces without altering bacterial viability, providing a novel approach for the management of biofilm formation on biomaterials.