Trujilloagerskov2984

An analytical method has been developed and validated for determining 107 pesticide residues in dried red pepper using LC-MS/MS. LC method, the clean-up and sample dilution processes were examined to determine their impact on reducing the matrix effects. Clean up was performed using an ENVI-CarbIITM/PSA (300/600 mg, 6 mL) SPE cartridge. In the sample dilution process, eight-fold dilution was used. In the validation of the developed method at two concentrations (0.01 and 0.1 μg/g) for 107 pesticides, 96 pesticides showed recovery rates in the range of 70.1 to 112.6%, RSDs of repeatability of ≤11.5 and 3.4%, and RSDs of within-laboratory reproducibility of ≤24.3 and 19.9%. These values fulfill the criteria of the validation guidelines for pesticide residues in Japan. It is concluded that matrix effects and low recovery rates in the process of extraction are the main factors for values that do not conform to the criteria.We developed a simple rapid analysis of multi-pesticide residues in agricultural products. In this study, we attempted to simplify the purification process, and reduce the amount and type of solvent used. The test solution was prepared by clean-up, a 0.5 mL aliquot of QuEChERS extract solution of agricultural products using a 3-layer solid-phase (C18/SAX/PSA) extraction mini-column, and the test solution was subjected to GC-MS/MS analysis, modified with a large volume injection and a stomach-type glass-lined injector. This method met the acceptability criteria of recovery (70-120%) and standard deviation of repeatability (RSD less then 25%) in 241-331 pesticides in 8 types of agricultural products.To quantify the amount of authorized GM maize or soybean, conversion factor (Cf) values are required for converting the copy number ratio of GM sequence to an endogenous sequence into weight-based GMO amounts. Cf values are available for the several latest real-time PCR instruments such as QuantStudio5, QuantStudio12K Flex, LightCycler 96, and LightCycler 480 for GM soybeans but not for GM maize. For the quantification of GM maize, we experimentally determined the Cf values targeting Cauliflower mosaic virus 35S promoter (P35S), GA21 construct specific, MIR604 event specific and MIR162 event specific sequences using the four real-time PCR instruments.The Japanese official analysis method for determination of nitrate ions in food products used as food additives is associated with various challenges. In some kinds of cheese, the extract becomes suspended. The volume of extracted solution is often not accurate owing to the presence of residues in the solution. Moreover, the determination with liquid chromatography-ultraviolet detection (HPLC-UV) is difficult owing to the influence of impurities. Sake usually does not contain lipids or proteins ; therefore, its analysis can be simplified by omitting the co-precipitation steps to remove them. In the present study, for cheese, the amount of sodium hydroxide solution that causes suspension was reduced, and the influence of residues was removed by adjusting the volume after suction filtration. Whereas, sake was diluted with water and centrifuged. Furthermore, solid-phase extraction (SPE) method using cartridge containing carbon molecular sieve to remove the influence of impurities on the chromatogram was successfully established. The recoveries of the nitrate ions were good outcomes of 91.3-99.6% (CV 0.9-4.5%) (n=5). The analysis range was 0.010-0.20 g/kg for cheese, 0.010-0.20 g/L for milk, and 0.010-0.10 g/kg for sake. The developed analysis methods are considered useful, because various challenges of the official analysis method can be solved and the operation are efficient.A determination method for tributyltin (TBT) and triphenyltin (TPT) in fish and shellfish using an accelerated solvent extractor (ASE) and LC-MS/MS was developed. The chromatographic separation was conducted on a Poroshell 120 EC-C18 column using an isocratic mobile phase of 0.1% formic acid in 70% methanol. Sample preparation was performed using ASE at 125℃ with n-hexane and a cleanup using a Florisil cartridge. Internal calibration curves using deuterium-labeled TBT and TPT were employed for quantification. For both TBT and TPT, the calibration curves were linear in the range of 0.2-250 ng/mL, and the method quantification limits were 0.8 ng/g for both TBT and TPT. A National Institute for Environmental Studies certified reference material, No. 15 (adductor muscle of scallop), was analyzed to assess the performance of the developed method. The trueness, relative standard deviations of repeatability, and within laboratory reproducibility of this method, evaluated using a recovery test with four spiked fish species and one shellfish, ranged from 89.3 to 105.3%, 1.0 to 4.5%, and 1.3 to 7.6%, respectively.The fate of organophosphorus flame retardants (PFRs) in polished rice during the cooking process was examined. After adding PFRs to polished rice (500 ng/g), the rice was washed with water and cooked in a rice cooker. Among the seven kinds of PFRs, approximately 68-86% of the PFRs residues were removed after washing with water three times. In the first washing with water, approximately 50-70% of the PFRs residues were removed from rice. By the subsequent heating in an electric rice cooker or in a microwave rice cooker, the removal rates of PFRs in the cooked rice were 74-94 and 78-96%, respectively. The removal rates in musenmai (500 ng/g) which need not to wash before cooking were lower than those in the washed rice. https://www.selleckchem.com/products/diphenhydramine.html In the cooked musenmai, 10-50% of the PFRs residues were removed after heating in the electric rice cooker, and 10-70% were removed in the microwave rice cooker. The washing process is effective to reduce the intake of PFRs from rice.To identify the risk of Salmonella in meat, we investigated the prevalence of Salmonella, serovars and their antimicrobial susceptibility patterns. Salmonella was found in 353 out of 849 (41.6%) chicken, 9 out of 657 (1.4%) pork, 1 out of 517 (0.2%) Beef, 6 out of 8 (75.0%) chicken offal, 43 out of 142 (30.3%) pork offal and 4 out of 198 (2.0) beef offal samples collected from retail meats in Tokyo, Japan between 2009 and 2017. Salmonella Infantis was the most common serovar, followed by S. Schwarzengrund in the isolates from domestic chicken meats. The prevalence rate of S. Infantis decreased while that of S. Schwarzengrund increased by the year. Apart from this, the most prevalent serovars were S. Heidelberg in the imported chicken meat isolates, S. Typhimurium and Salmonella O4i- in pork, and S. Derby in beef isolates. Antimicrobial testing revealed high resistance to tetracycline (TC) in all meat sample isolates; however, all the isolates were sensitive to carbapenem and fluoroquinolone. Fourteen cefotaxime (CTX) resistant strains, seven extended spectrum β-lactamase (ESBL) producing strains and twenty-three AmpC producing strains were isolated from chicken meat samples.