Mullinsmccarty8774

Haematological parameters were analysed using a sysmex haematology analyser. The results of this study showed that the infection adversely affected the biomarkers examined showing its negative effect on liver, kidney, haematological parameters and host electrolyte balance. Treatments with Vitamin E was however able to mitigate the negative effect of this infection. In conclusion, the treatment was able to ameliorate the anaemia and organ damage caused by Trypanosoma brucei brucei, extend the life span of the treated rats and greatly delay the time taken to get to the second stage of the infection.Mice infected with T. evansi cause various clinical manifestations and histopathological changes. The aim of this study was to compare the histopathological lesions of mice infected with T. evansi Bang 87 isolates (high virulence) and Pml 287 isolates (low virulence). A total of 15 susceptible mice (DDY) were divided into three groups (five mice/group) Groups I and II each were infected with 104 T. evansi of high virulence (Bang87) and low virulence (Pml 287), respectively, whereas group III served as a control group. A total of three mice from group I, and one mouse from each group II and III were killed at 4 dpi. A total of two mice from each group II and III were killed at 24 dpi. Two remaining mice from each group were observed until succumb. Mice of group I and group II at 4 dpi showed no gross lesions. However, mice of group I showed very acute animal death at 5 dpi and showed mild to moderate histopathological lesions at 4 dpi, namely non-suppurative encephalitis, non-suppurative pneumonia, hepatitis non-suppurative with intravascular trypanosomiasis, tubular degeneration and necrosis. Group II showed chronic death at 26 dpi with significant gross pathological changes at 24 dpi in spleen (swelling 10 times than normal size) accompanied by severe non-suppurative encephalitis, cholangiohepatitis non-suppurative and bile duct proliferation, diffused splenic necrosis. The result of this study is expected to be used as a basis for improved treatment management in cattle infected with high virulence T. evansi isolates that are need to be handled appropriately to avoid fatal consequences.Bioindicator value of digenean parasites (Acanthostomum absconditum, Acanthostomum spiniceps and Haplorchoides cahirinus) of silver catfish, Bagrus bajad was assessed by analyzing the concentrations of nine heavy metals (Fe, Zn, Cu, Ni, Cd, Cr, Mn, Co, and Pb) using atomic absorption spectrometry. Additionally, the study compared heavy metals concentrations in four sinks (water, gills, intestine and digenean worms) from two aquatic habitats (the upstream and downstream) of the Damietta Branch of the River Nile from January to December, 2018. The results revealed that iron and manganese were the most abundant metals in water, gills, intestine and digenean worms at both the upstream and downstream aquatic habitats. In addition, the digenean worms of B. bajad inhabiting these two localities accumulated higher amounts of all analyzed heavy metals than water, gills and intestine (especially iron and manganese), except for chromium, the highest level of which was encountered in the gill tissues. The seasonal variations of heavy metals indicated their highest concentration in summer (for water, gills, intestine and digenean worms) and lowest concentration in autumn and winter (for water, gills and intestine), and in winter and spring (for digenean parasites). The results confirmed that the digenean intestinal parasites accumulate heavy metals pollutants in a significant manner and are important model for monitoring pollution.Opisthorchis viverrini infection causes various complications in patients, ranging from asymptomatic to severe chronic disease including cholangiocarcinoma (CCA). O. viverrini is endemic in Southeast Asia and acting as a risk for CCA. see more Early diagnosis of O. viverrini infection can reduce the number of CCA cases. The routine diagnosis for opisthorchiasis is direct wet smear, sometimes coupled with concentration techniques, which has limitations when investigating light infection or if done by laboratorians with lack of experiences. PCR-based methods have been established for the detection of O. viverrini egg DNA from stool samples, but have never fully succeeded for light infections when compared to wet smear concentration techniques. This study aims to improve the PCR-based method for detection of O. viverrini eggs in stool samples by targeting the genes ITS-2, cox1, and cyb. The results reveal higher sensitivity than conventional concentration techniques, with all newly designed primers. ITS-2 has an overall sensitivity of 76.9% with 66.7% in the samples with less then  50 EPG, while cox1 has shown 96.2% overall sensitivity and 94.1% in the same EPG intervals. Interestingly, the new pointing target, cyb, has shown 100% sensitivity in all egg intervals in this study, particularly for light infections (EPG less than 100). No cross-reactivity was found in Taenia spp., Trichuris trichiura, Ascaris lumbricoides, Capillaria philippinensis, and hookworm. The procedure is convenient, with shorter steps compared to previous reports, and it appears appropriate for use in the diagnosis of light infection with O. viverrini. These three genes are good candidates for use in PCR-based detection of the parasite eggs. Further testing with a larger cluster of samples is however necessary.The cytotoxicity of Acanthamoeba is yet to fully illustrate due to recalcitrant of Acanthamoeba during cyst stage. The formation of the trehalose layer at the cyst stage protects the inner components of this opportunist protozoan parasite. Trehalase from the Aspergillus niger (AnTre) activity on the cyst of Acanthamoeba was determined based on AnTre dose-response, morphological and protein changes. The interaction of the AnTre and trehalose was also visualized through docking simulation. Vacuolation of the cyst can be seen when observed under light microscopy. Membrane integrity assessment suggested possible hydrolization of the AnTre enzyme to trehalose membranes which based on acridine orange and propidium iodide staining. Surface morphology based on scanning electron microscopy revealed the formation of bulging structure that was also proved through cross sectioning observed by transmission electron microscopy. Loss of internal structure of the cysts was clearly observed. Other morphological distinction where loss of rigid shape due to the destruction of the endo- and ecto cyst layers.