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Genera Acinetobacter, Pseudomonas, Corynebacterium_1 and Proteiniphilum were major potential hosts for multiple genes. The ARG, MRG and MGE profiles were mainly driven by the joint effect of environmental factors and microbial community.Increased utilization of silver nanoparticles (AgNPs) can result in an accumulation of these particles in the environment. The potential detrimental effects of AgNPs in soil may be associated with the low fertility of soils in semiarid regions that are usually subjected to restoration through the application of organic amendments. Microbial communities are responsible for fundamental processes related to soil fertility, yet the potential impacts of low and realistic AgNPs concentrations on soil microorganisms are still unknown. We studied the effects of realistic citrate-stabilized AgNPs concentrations (0.015 and 1.5 μg kg-1) at two exposure times (7 and 30 days) on a sandy clay loam Mediterranean soil unamended (SU) and amended with compost (SA). We assessed soil microbial biomass (microbial fatty acids), soil enzyme activities (urease, β-glucosidase, and alkaline phosphatase), and composition of the microbial community (bacterial 16S rRNA gene and fungal ITS2 sequencing) in a microcosm experiment. In the SA, the two concentrations of AgNPs significantly decreased the bacterial biomass after 7 days of incubation. At 30 days of incubation, only a significant decrease in the Gram+ was observed at the highest AgNPs concentration. In contrast, in the SU, there was a significant increase in bacterial biomass after 30 days of incubation at the lowest AgNPs concentration. Overall, we found that fungal biomass was more resistant to AgNPs than bacterial biomass, in both SA and SU. Further, the AgNPs changed the composition of the soil bacterial community in SA, the relative abundance of some bacterial taxa in SA and SU, and fungal richness in SU at 30 days of incubation. However, AgNPs did not affect the activity of extracellular enzymes. This study demonstrates that the exposure time and organic amendments modulate the effects of realistic concentrations of AgNPs in the biomass and composition of the microbial community of a Mediterranean soil.This study presents systematic investigations to evaluate the performance, rejection rate, fouling, cleaning protocols and impact of physical and chemical cleaning strategies on the performance of commercial cellulose triacetate (CTA) membrane. The treatment of landfill leachate (LFL) solution was performed in the active layer facing feed solution and support layer facing the draw solution (AL-FS mode), and active layer facing the draw solution and support layer facing the feed solution (AL-DS mode). Compared to the AL-FS mode, a higher flux for AL-DS mode was achieved, but membrane fouling was more severe in the latter. In both membrane orientations, the rejection rate of the FO membrane to heavy ions and contaminants in the wastewater was between 93 and 99%. Physical and chemical cleaning strategies were investigated to recover the performance of the FO membrane and to study the impact of cleaning methods on the membrane rejection rate. Physical cleaning with hot water at 35 °C and osmotic backwashing with 1.5 M NaCl demonstrated excellent water flux recovery compared to chemical cleaning. In the chemical cleaning, an optimal concentration of 3% hydrogen peroxide was determined for 100% flux recovery of the fouled membrane. However, slight membrane damage was achieved at this concentration on the active layer side. Alkaline cleaning at pH 11 was more effective than acid cleaning at pH 4, although both protocols compromised the membrane rejection rate for some toxic ions. A comparison of the membrane long-term performance found that cleaning with osmotic backwashing and hot water were effective methods to restore water flux without comprising the membrane rejection rate. Overall, it was found that physical cleaning protocols are superior to chemical cleaning protocols for forward osmosis membrane fouled by landfill leachate wastewater.Extra-Andean Patagonia, located in the south end of South America, is a wide arid region where the basaltic plateau landscape dominates. Associated with the basaltic plateaus, wet meadows and shallow lakes make up environments of relevance due to the ecosystemic services they provide. The aim of this work was to define the processes and factors that condition the hydrodynamics and hydrogeochemistry of wet meadows and shallow lakes in a sector of the Patagonian plateau basalts. For this, detailed geological and geomorphological studies were carried out and water was sampled at 29 points for the analysis of major ions and, in some samples, stable isotopes of the water molecule. Values of δ18O and δ2H similar to the local meteoric line in the water of wet meadows indicate that rainwater quickly infiltrates through the fractures of the basaltic plateaus and stores in the underlying sediments. Groundwater discharge occurs in the scarp zone due to changes in sediment permeability. The weathering of silicates in basalts, ion exchange in the sediment clays, and the dissolution of soluble salts provided by the dust control water chemistry of this wetland. Water from wet meadows drains into saline endorheic basins of sodium chloride composition where the isotopic signal is indicative of evaporation percentages close to 95%. The high evaporation rates cause the precipitation of evaporitic salts at the edges of shallow lakes with carbonate, sulfate, and chloride species controlled by the dominance of sodium in the water.Pharmaceuticals and other emerging contaminants (EC) have been increasingly detected and measured in coastal waters and large effort has been devoted to knowing the effects these substances have in coastal ecosystems. Anthozoa class is underrepresented in ecotoxicology studies despite some of their species being endangered. Anemonia sulcata and Actinia equina are species widely distributed in the Mediterranean Sea. The objectives of this work have been to evaluate the ability of these species to accumulate carbamazepine (CBZ) from water, to determine the effects of this pharmaceutical on some physiological and biochemical endpoints and to characterize the degradation routes followed by this compound in Anthozoa tissues (biotransformation) and water. Sea anemones were exposed to 1 μg L-1 and 100 μg L-1 of CBZ in artificial sea water in a semi-static system for 8 days. At several times small portions of the tentacles and whole organisms were taken. Ion transport (measured as NKATPase activity), energetic metabolism (measured as glucose and lactate levels) and nitrogen excretion (measured as ammonia concentration in tissues) were determined. CBZ-exposed individuals of A. sulcata and A. Oxaliplatin equina were analyzed by ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS) on a quadrupole-time-of-flight (QqTOF). The structures of nine metabolites have been tentatively identified using HRMS and HRMS/MS data with the aid of the free available Medline database. The current work constitutes the first study on the identification of Cnidarian metabolites of CBZ in species of the Anthozoa class.Nowadays, the use of constructed wetlands for on-site greywater treatment is a very promising option. The successful application of this nature-based solution at full scale requires public acceptance, economic feasibility and the production of high-quality treated greywater. This work focuses on the use of ornamental plants as vertical flow constructed wetland (VFCW) vegetation for greywater treatment, aiming to improve aesthetic and acceptability of the system. The performance and economic feasibility of the proposed green technology were examined during a 2-years study. Results show that Pittosporum tobira and Hedera helix can grow in VFCW operating with greywater without any visible symptoms. These species tolerated both drought and flooding conditions, making them ideal for use not only in residential buildings but also in seasonal hotels and holiday homes. In contrast, partial defoliation of Polygala myrtifolia plants was observed during the winter period. High average removal efficiencies were observed for BOD (99%), COD (96%) and TSS (94%) in all examined VFCWs including unplanted beds. Phosphorus removal gradually decreased from 100% during first months of operation to 15% during second year of operation. In addition, total coliforms concentration reduced by 2.2 log units in the effluent of all planted systems, while lower removal efficiency was observed in the absence of plants. The mean concentration of BOD and TSS in the treated greywater met the standards for indoor reuse ( less then 10 mg/L). Cost payback periods for the installation of the proposed technology in a multi-family building, a single house and a hotel in Greece were found 4.7, 16.6 and 2.5 years, respectively. Overall, the "treatment gardens" proposed in this study provide a technically and economically feasible solution for greywater treatment, with the additional benefit of improving the aesthetic of urban, semi-urban and touristic areas.Intensive aquaculture significantly affects the global phosphorus (P) cycle and enhances eutrophication in inland waters. Sediment dredging efficiently removes P-rich sediments from shallow-water eutrophic lakes. However, studies on the effects of sediment dredging on the internal P loading of aquacultural lakes are still lacking. Moreover, the migration and transformation processes of labile P and the mechanisms of sediment P release are unclear. To evaluate dredging effectiveness, we employed two in situ high-resolution sampling techniques to simultaneously measure sediment labile P and porewater soluble reactive P (SRP) and Fe (II) at the millimeter scale. Dredging effectively reduced surface sediment CaP contents and organic matter (OM) below the sediment-water interface (SWI). Moreover, dredging decreased the SRP diffusion flux across the SWI in summer. After dredging, FeP (P bound to Fe, Al, and Mn oxides and hydroxides) and OP (organic P) contents increased by 136% and 48% in the newly formed deposited layer (140 mm thick), respectively. The increased bioavailable P content significantly enhanced the capability of sediment solids to resupply labile P to porewater SRP. The stronger positive correlation between porewater soluble Fe (II) and SRP suggests that Fe redox cycling regulated internal P release. Our results suggest that dredging effectiveness will weaken over time due to the re-deposition of active P, which in turn increases the risk of sediment P release. To curb the release of sediment P, we recommend the implementation of additional in situ restoration techniques that improve the oxide layer of surface sediments and reduce sediment suspension.Veterinary antibiotics reaching the environment have become a matter of global concern, since they can cause serious negative impacts on human and ecological health. Therefore, a deep understanding of their behavior and fate once they reach the soil environment is of utmost importance to design and implement appropriate measures that could reduce their potential risks. With this aim, batch-type experiments were carried out to study competitive adsorption and desorption for three sulfonamide antibiotics (sulfadiazine -SDZ-, sulfamethazine -SMT-, and sulfachloropyridazine -SCP-) in six crop soils presenting different characteristics. The results obtained showed that sulfonamides have a low retention in soils, with average adsorption percentages of 40% for SDZ, 44% for SMT and 54% for SCP, and with desorption percentages up to 36% for SDZ and SCP and up to 29% for SMT. The retention of sulfonamides was strongly influenced by the soil organic carbon content (SOC), with higher adsorption and less desorption associated to higher SOC contents.

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