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The classification of wastes regarding hazardous property HP 14 (ecotoxicity) is essential for proper waste management. In the EU, HP 14 has been estimated based on waste chemical composition rather than using biotests, and guidelines for experimental assessment are still lacking. This study aims at evaluating the potential ecotoxicological impacts of weathered coal fly ash (CFA) from a landfill, as a case study to assess the current EU methodology used to classify wastes regarding HP 14. A large amount of CFA is still landfilled, but its valorisation would be of interest. The analysis was based on the chemical composition of CFA (in ClassifyMyWaste software), and on a battery of five biotests applied to eluates, with Lepidium sativum, Aliivibrio fischeri, Raphidocelis subcapitata, Lemna minor and Daphnia magna. Through chemical analysis, most of the simulations with data from the literature indicated "Possible Hazard", including the sample of this work. Biotests revealed low impairment for most endpoints. D. magna was the most sensitive organism, but the inhibitory effect was significantly reduced after pH adjustment of the eluate. The test with A. fischeri does not seem to be adequate to assess CFA due to the high variability observed in results. The methodology involving a simple battery of bioassays was proven to be enlightening, providing relevant results for HP 14 assessment. The chosen battery of biotests (excluding the A. fischeri test) may be a good starting point to represent the aquatic environment in this context. In short, it seems that weathered CFA can be considered non-hazardous, and therefore the material under analysis could be valorised in practical applications without significant ecotoxic effect on the environment.Vegetable oils are characterized by their bioactive phytochemicals including fatty acids, tocols, and phenolic compounds. In the current study, turnip (Brassica rapa) oil was evaluated for its fatty acid profiles, tocol composition, and total phenolic content. The radical scavenging properties of oil against DPPH· and galvinoxyl radicals were also evaluated. Turnip oil efficiency in treating osteoporosis was tested in rats. Fifty adult female Sprague-Dawley albino rats were divided to five groups (n = 10/group). An osteoporotic rat model was prepared by two separate 5-day (5 days on/9 days off) courses of methotrexate subcutaneous injection. Osteoporotic rats were orally gavaged with turnip oil (200 and 400 mg/kg/day) for 28 days. Turnip oil efficiency in treating osteoporosis was studied by evaluation of Osterix, Cath K, and TNF-α transcript expression levels that involved in bone remodeling in femoral bones. Minerals and vitamin D were estimated in blood serum. Femoral bone histological and morphometric analyses were investigated in osteoporotic and turnip oil-treated rats. In vitro assays revealed strong antiradical potential of turnip oil. Treatment with turnip oil regulated the levels of Osterix, Cath K, and TNF-α mRNA that was accompanied with elevating the levels of calcium, phosphorous, bone alkaline phosphatase (BALP), and vitamin D in osteoporotic rats. The histological and morphometric inspection revealed that turnip oil displayed progress in the osteoporotic rat bone formation that was clear in the enhancement of thickness of femur shaft cortical bone and femur head trabecular bone. Above-mentioned findings indicated that turnip oil has the potential to share in the treatment of osteoporosis.Recently, Dr. Yuh-shan Ho discussed the search methods of the paper "Dynamic analysis of international green behavior from the perspective of the mapping knowledge domain" Li et al. (Environ Sci Pollut Res 266087-6098, 2019a). In fact, Ho and co-workers have used the search methods in several papers Ivanović et al. (Scientometrics, 105145-160, 2015); Monge-Nájera & Ho (Rev Biol Trop 631255-1266, 2015); Ho & Hartley (Br J Psychol, 107768-780, 2016). In order to reply to the comments, this letter supplementarily explains the characteristics, scope, and limitations of search methods. In addition, the letter states that researchers can use improved methods suggested by Dr. Ho in future studies.Heavy metal pollution is a global ecological safety issue, especially in crops, where it directly threatens regional ecological security and human health. Selleckchem GSK591 Selecting scientific evaluation methods is an important prerequisite for understanding the distribution of heavy metals in a region. To evaluate the distribution characteristics of arsenic (As), cadmium (Cd), copper (Cu), and zinc (Zn) in farmland soil-rice system in Doumen District of Zhuhai City, Pearl River Delta, we analyzed the high geological background area and heavy metal contents in soil by inverse distance-weighted interpolation and single-factor pollution index. Bioconcentration factor (BCF) was used to study the migration and accumulation characteristics of heavy metals. Then, the soil-rice system was evaluated comprehensively with a novel evaluation method, i.e., the influence index of comprehensive quality (IICQ). Results showed that As, Cd, Cu, and Zn in the soil of the study area followed normal distribution. Cd and Cu were the main pollutants whose point contamination rates were 50% and 22.86%, respectively. A total of 2.86% of the soil were contaminated by As, and no Zn contamination was observed in the soil. At the same time, As and Cd in rice were partially polluted, and the Cu and Zn were not polluted. The order of bioaccumulation coefficient was Cd > Zn > Cu > As, and no evident enrichment was observed. According to the impact index of IICQ to evaluate the pollution of heavy metals in the soil-rice system, 96.98% of the soil in study area was in a state of moderate, heavy, and extreme pollution, which were concentrated in the northern and central parts of the study area. The soil-rice system in the high geological background area was in a subhealthy state. A total of 90.69% of the soil were polluted, but the rice met the national food safety standards.Environment-biased technological progress plays a critical role in carbon reduction, while the association among environment-biased technological progress, energy consumption, and carbon emissions has not been paid enough attention. Working with a unique spatial panel dataset of APEC economies spanning the 2000-2017 period, we employed the nonspatial panel model and the spatial panel model to investigate the role of fossil energy (FE) and clean energy (CE) consumption in carbon dioxide (CO2) abatement through environment-biased technological progress (EBTP). We decomposed EBTP into both emission-reducing biased technological progress (ErBTP) and energy-saving biased technological progress (EsBTP). The results show that the direct effect of EBTP on CO2 emissions was significantly negative and that the direct effect of ErBTP was significantly larger than that of EsBTP. EBTP reduced CO2 emissions through CE consumption, whereas it increased CO2 emissions through FE consumption, that is, EBTP had a "backfire effect" on FE consumption.

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