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Moreover, SWCNT-COOH interacted strongly with the N-terminal region, turn region and C-terminal region of the Aβ40 trimer via hydrogen bonds, salt bridges and π-π interactions, which triggered a large structural disturbance of the Aβ40 trimer, reduced the β-sheet content of the Aβ40 trimer and led to more disorder in these regions. All the above data not only reveal the suppressive effect of SWCNT-COOH on Aβ aggregation, but also reveal its inhibitory mechanism, which provides a useful clue to exploit anti-Aβ drugs in the future.Since the nanotoxicity of gene delivery carriers has raised world-wide concerns, it is important to trace their intracellular performance, for example via uptake visualization. Here, we develop a novel ultrathin graphitic carbon nitride (g-C3N4) composite nanosystem for label-free Raman-traceable small interfering RNA (siRNA) delivery. Through low molecular weight polyethylenimine (PEI) modifications, these nanosystems can obtain siRNA loading capabilities. The lateral size of the PEI-g-C3N4 composite is around 100-150 nm with a thickness of nearly 0.6 nm. The designed label-free delivery system could avoid possible obstacles associated with artificial labels and it shows cytotoxicity toward cancer cells and good biocompatibility in normal human cells. The label-free PEI-g-C3N4 gene nanocarrier can be directly traced via Raman microscopy, which makes it suitable for intracellular visualization. Intracellular uptake of the self-fluorescent g-C3N4 nanosheets can also be traced via fluorescence imaging. The PEI modified g-C3N4 ultrathin nanosheets possess gene delivery capacity together with unique dual-traceable Raman and fluorescence features. Raman traces not only have higher specificity than fluorescence ones but they can also avoid background noises. Thus, they may replace widely implemented fluorescence tracing. This work could provide a label-free traceable platform for investigating the intracellular performances of gene delivery nanosystems.Extracellular matrix (ECM)-based materials have been employed as scaffolds for bone tissue engineering, providing a suitable microenvironment with biophysical and biochemical cues for cell attachment, proliferation and differentiation. In this study, bone-derived ECM (bECM)-incorporated electrospun poly(ε-caprolactone) (PCL) (bECM/PCL) nanofibrous scaffolds were prepared and their effects on osteogenesis were evaluated in vitro and in vivo. Dizocilpine The results showed that the bECM/PCL scaffolds promoted the attachment, spreading, proliferation and osteogenic differentiation of rat mesenchymal stem cells (MSCs), mitigated the foreign-body reaction, and facilitated bone regeneration in a rat calvarial critical size defect model. Thus, this study suggests that bECM can provide a promising option for bone regeneration.Respiration rate is a vital parameter which is useful for the earlier identification of diseases. In this context, various types of devices have been fabricated and developed to monitor different breath rates. However, the disposability and biocompatibility of such sensors and the poor classification of different breath rates from sensor data are significant issues in medical services. This report attempts to focus on two important things the classification of respiration signals from sensor data using deep learning and the disposability of devices. The use of the novel Janus MoSSe quantum dot (MoSSe QD) structure allows for stable respiration sensing because of unchanged wear rates under humid conditions, and also, the electron affinity and work function values suggest that MoSSe has a higher tendency to donate electrons and interact with the hydrogen molecule. Furthermore, for the real-time classification of different respiration signals, a 1D convolutional neural network (1D CNN) was incorporated. This algorithm was applied to four different breath patterns which achieved a state-of-the-art 10-trial accuracy of 98.18% for normal, 95.25% for slow, 97.64% for deep, and 98.18% for fast breaths. The successful demonstration of a stable, low-cost, and disposable respiration sensor with a highly accurate classification of signals is a major step ahead in developing wearable respiration sensors for future personal healthcare monitoring systems.The poor mechanical properties of wound dressings have always been a challenge in their application as wound protective barriers. In particular, when the hydrogel dressing absorbs the tissue fluid, the mechanical properties of the hydrogel will decrease greatly due to the swelling effect. In this study, an original antibacterial hydrogel dressing was prepared by a one-step process with acrylic acid, 1-vinyl-3-butylimidazolium, COOH-modified gum arabic, and aluminium chloride. The mechanical properties of this hydrogel were improved after water absorption due to hydrophobic interactions, so the hydrogel dressing could maintain good mechanical properties after absorption of the tissue fluid. Furthermore, 1-vinyl-3-butylimidazolium as an ionic liquid was introduced into the polymer backbone of hydrogels via covalent bonds and could promote the self-healing of hydrogels by facilitating the migration of aluminum ions with charge. The obtained hydrogels showed good self-healing properties, with a strain self-healing rate of 98.2% and a stress self-healing rate of 92.3%. In addition, this hydrogel exhibited excellent antibacterial activity against E. coli, S. aureus, and C. albicans. The results of the study on rat wound closure indicated that this hydrogel effectively accelerated the healing of a full-thickness skin defect. Therefore, this novel hydrogel has a broad application prospect in the field of wound dressing.This work reports a photoelectrochemical (PEC) biosensing platform for the sensitive and specific screening of thrombin by using graphene oxide-coated copper-doped zinc oxide quantum dots (Cu0.3Zn0.7O-GO QDs) as the photoactive materials and glucose oxidase-encapsulated DNA nanoflowers (GOx-DFs) for signal amplification. Interestingly, the coated graphene oxide nanosheets on the surface of the Cu0.3Zn0.7O QDs could cause the charge to transfer rapidly and ameliorate the photocorrosion. The doped copper into the quantum dots could enhance the absorption of visible light by tuning the band gap of ZnO QDs, therefore increasing the photocurrent under visible irradiation. Upon addition of target thrombin, a sandwiched reaction was carried out between thrombin aptamer and GOx-DFs, accompanying the formation of nanocomposites with the magnetic microparticles (MMPs)/thrombin/GOx-DFs. Followed by magnetic separation, the carried GOx oxidized glucose to H2O2, thus resulting in the increasing photocurrent of the Cu0.3Zn0.7O-GO QD-modified electrode. Under optimum conditions, the developed PEC biosensing platform exhibited good analytical performance with a linear range of 50-10 000 fM thrombin and a limit of detection of 29 fM. Impressively, our strategy offers a new horizon in developing bridge-connected graphene-coated nanomaterials and novel signal amplification strategy for the development of PEC biosensors.Using ferrocenecarboxylic acid (FcCO2H) and triethanolamine (H3tea) as ligands, the isostructural heterotrimetallic complexes [LnIII2CrIII2(OH)2(FcCO2)4(NO3)2(Htea)2]·2MePh·2THF (Ln = Tb (1), Dy (2), Ho (3), Er (4), and Y (5); Fc = (η5-C5H4)(η5-C5H5)Fe; H3tea = N(CH2CH2OH)3) were obtained. In all of the complexes which possess a defective dicubane structure, two doubly deprotonated triethanolamine ligands chelate the chromium ions. However, during the synthesis of 1, an isomeric complex 1a in which Tb3+ is chelated by triethanolamine as a tetradentate ligand, was also isolated as a few single crystals. Magnetic susceptibility measurements revealed dominant antiferromagnetic interactions in the LnIII2CrIII2 cores of 1-4 leading to the formation of complexes with an uncompensated magnetic moment, while weak Cr-Cr ferromagnetic interactions were detected in the Y analogue. Complexes 1, 2, and 3 exhibit single-molecule magnet properties dominated by an Orbach-type relaxation mechanism with magnetization reversal barriers (Δ/kB) estimated around 54, 75, and 47 K, respectively. The Dy complex exhibits a magnetization hysteresis in an applied magnetic field at temperatures below 4 K. Thermolysis of the complexes was studied by TGA and DSC techniques; the final products obtained under an air atmosphere contain mixed oxide Cr0.75Fe1.25O3 and heterotrimetallic oxide LnCr1-xFexO3 (with x ≈ 0.75) phases.Covering through June 2021Terpenoids are the largest class of natural products recognised to date. While mostly known to humans as bioactive plant metabolites and part of essential oils, structurally diverse terpenoids are increasingly reported to be produced by microorganisms. For many of the compounds biological functions are yet unknown, but during the past years significant insights have been obtained for the role of terpenoids in microbial chemical ecology. Their functions include stress alleviation, maintenance of cell membrane integrity, photoprotection, attraction or repulsion of organisms, host growth promotion and defense. In this review we discuss the current knowledge of the biosynthesis and evolution of microbial terpenoids, and their ecological and biological roles in aquatic and terrestrial environments. Perspectives on their biotechnological applications, knowledge gaps and questions for future studies are discussed.To unearth suitable complexes that are capable of inhibiting the growth of MDA-MB-468 and Caco-2 cells, 2,2'-bipyrimidine-based luminescent Ru(ii)/Ir(iii)-arene monometallic and homo- and hetero-bimetallic complexes were synthesized. The complex [(η6-p-cymene)(η5-Cp*)RuIIIrIIICl2(K2-N,N-bipyrimidine)](PF6)2 [LRuIr] exhibited the best potency in both cells along with good GSH stability and strong binding efficacy with the biomolecules. The apoptotic event occurred in MDA-MB-468 cancer cells via cell cycle arrest.A new porous metal-organic framework (MOF) with the chemical formula [Y5L6(OH)3(DMF)3]·5H2O (1) (where L = 3-amino-4-hydroxybenzoate) has been prepared by a solvothermal procedure. The structural characterization reveals that this material consists of a robust three-dimensional metal-organic framework (MOF) grown with clusters formed by Y(iii) and hydroxide anions joined to one another by the ligand, giving rise to an open structure with interconnected microchannels with variable dimensions. This assembled set has shown to possess a fascinating catalytic activity for the cyanosilylation of a broad range of aldehydes and ketones with exceptional recyclability, a solvent-free medium, and one order of magnitude lower catalyst loading compared to all related lanthanide-based MOFs described so far in the literature.The stoichiometric reactions of antimony trichloride, trimethylsilyl trifluoromethanesulfonate, and diiminopyridine ligands lead to the formation of N,N',N-chelated SbCl2 cationic complexes. Methyl and phenyl substituents on the imine carbons of the ligand yielded structures with a lone pair on antimony and the hydrogen substituted variant was notably different as it forms a Menshutkin complex with meta-xylene in the solid-state.