Milnealbrektsen4918
Selenite biotransformation by microorganisms is an effective detoxification and assimilation process. Bacillus subtilis is a probiotic bacterium that can reduce Se(IV) to SeNPs under aerobic conditions. However, current knowledge on the molecular mechanisms of selenite reduction by B. subtilis remains limited. Here, the reduction of Se(IV) by probiotic bacterium Bacillus subtilis 168 was systematically analysed, and the molecular mechanisms of selenium nanoparticle (SeNPs) formation were characterised in detail. B. subtilis 168 reduced 5.0 mM selenite by nearly 40% in 24 h, and the produced SeNPs were spherical and localised intracellularly or extracellularly. FTIR (Fourier transform infrared) spectroscopy suggested the presence of proteins, lipids, and carbohydrates on the surface of the isolated SeNPs. Transcriptome data analysis revealed that the expression of genes associated with the proline metabolism, glutamate metabolism, and sulfite metabolism pathways was significantly up-regulated. Gene mutation and complementation revealed the ability of PutC, GabD, and CysJI to reduce selenite in vivo. In vitro experiments demonstrated that PutC, GabD, and CysJI could catalyse the reduction of Se(IV) under optimal conditions using NADPH as a cofactor. To the best of our knowledge, our study is the first to demonstrate the involvement of PutC and GabD in selenite reduction. Particularly, our findings demonstrated that the reduction of Se(IV) was mediated by multiple pathways both in vivo and in vitro. Our findings thus provide novel insights into the molecular mechanisms of Se(VI) reduction in aerobic bacteria.A new adsorbent that combines mineral vermiculite with the yeast Saccharomyces cerevisiae, was used for Cd2+ removal. The influence of vermiculite presence on the toxic effects of Cd2+ to Saccharomyces cerevisiae yeast was evaluated as a function of the microorganisms' respiratory activity (CO2 production). The Cd2+ toxicity increased with prolonged exposure time reaching the LC50 value of 857 and 489 mg L-1 after 30 and 120 min, respectively. The yeast managed to bioaccumulate 25.0 ± 0.6 mg g-1 of Cd2+ at the initial Cd2+ concentration of 741.9 mg L-1; the maximum Cd2+ adsorption capacity of vermiculite reached 25 ± 5 mg g-1. The addition of the mineral decreased the cations toxic effect; the LC20 value in vermiculite absence attained approximately 200 mg L-1 after 30 min and decreased to 80 mg L-1 after 2 h, while in the bio-mineral system it was at the level of 435 ± 50 mg L-1 without a significant change in time. The mineral provided a superior living environment for the yeast by removing part of the cations, releasing essential microelements and providing a protective, clay hutch-like habitat for the cells.The demand and importance of fairness creams as a major cosmetic have increased significantly in recent years. However, some of these cosmetics contain heavy metals, hydroquinone and microorganism that can cause various health problems. Therefore, the aim of this study is to determine and examine the concentration of metals (i.e. Cd, Pb, Cr, Ni, Zn, Hg), hydroquinone and microorganisms in nine different fairness creams produced by local and international brands. The health risk assessment of the tested substances for consumers was accessed through systemic exposure dosage (SED), margin of safety (MoS), lifetime cancer risk (LCR), hazard quotient (HQ) and hazard index (HI). learn more The concentration of Zn and Hg were found the highest and measured in the range of 17.82-138.06 mg.kg-1 and 2.3-141 mg.kg-1, respectively. The concentrations of other metals were determined as 0.06-0.67 mg.kg-1 of Cd, 0.43-1.55 mg.kg-1 of Cr, 0.14-1.43 mg.kg-1 of Ni and 0.3-1.34 mg.kg-1 of Pb. HPLC results showed a significant presence of hydroquinone in the range of 0.12-7.2%. The total viable counts of cosmetic samples showed the substantial presence of microorganisms, and 44% of the collected samples surpassed the permissible limit of 100 cfu/g recommended by European Union. Many of the collected samples exceeded the MoS, HQ and HI tolerance limits. However, the LCR value in all samples was significantly higher than the acceptable limit. Therefore, it is advised to avoid overuse of these products in order to ensure human safety and reduce the risks to skin health.Electrokinetic in-situ chemical oxidation (EK-ISCO) has attracted much attention during remediation of organic contaminated soil. Oxidants in EK-ISCO brings high cost and negative effects on soil physicochemical properties. In this study, a novel approach of combined electrokinetic treatment and anode oxidation was investigated to remediate phenanthrene polluted soils without adding oxidants. The fabricated Ti4O7 acted as anode, and could generate •OH at the rate of 9.31 × 10-7 mol h-1 at current 5.10 mA cm-2 through direct H2O electrolysis. Electro-osmotic flow (EOF) was used to transport phenanthrene to anode for the subsequent degradation. Sandy soil, fluvo-aquic soil and red soil were selected as typical soil samples, because pH and buffer capacity were two important factors affecting the direction of EOF. Strategies were developed to regulate the direction of EOF, including adding CEM membrane, maintaining soil pH at 3.5-4.0 and mixing solution from anode and cathode chambers. After treatment, more than 81.9% of phenanthrene was removed without adding any oxidants, and the remediated soil had low toxicity for Lolium perenne growth based on 3-d cultivation results. The results indicated that EK-AO had the advantage of less energy consumption and superior environmental friendliness than traditional EK-ISCO.Given the wide applications of silver nanoparticles (AgNPs), it is necessary to evaluate their potentially adverse long-term effects. In this study, we performed a 100-day exposure of medaka fish to citrate and luminogens coated AgNPs and investigated the maternal transfer potentials and biodistribution of AgNPs. Following long-term AgNPs exposure, AgNPs were mainly distributed in the liver, followed by gills, intestine, and brain, but were also detected in the ovary and strongly colocalized with the dissolved Ag+. The quantified transfer efficiency of different Ag species was 1.56-5.07%. Long-term exposure of medaka to small size of AgNPs (20 nm) reduced the hatching rate attributable to the accumulation of AgNPs and their dissolved Ag+. The maternally transferred AgNPs were mainly concentrated in the Kupffer's vesicle of embryos, while their dissolved Ag+ was almost homogeneously distributed in the embryos. In contrast, the newly accumulated AgNPs were mainly absorbed at the chorion of embryos. During initial larval development, the maternally transferred AgNPs and their dissolved Ag+ were consistently concentrated in intestine. Significant dissolution of maternally transferred AgNPs occurred during larval development. Our results showed that long-term exposure to AgNPs caused distinct biodistribution in the next generation of medaka, and may have implications for assessing their potential adverse effects.
The most commonly used apnea test for determination of brain death is the apneic oxygenation method. While all the commonly used apnea tests have associated complications, artificial CO
augmentation has been reported to have fewer complications. However, data supporting the use of the latter method is limited.
We identified 102 patients who underwent brain death testing during the last ten years. Artificial CO
augmentation apnea testing was used in 50 of these patients while 52 patients underwent standard apneic oxygenation apnea testing.
No significant differences between the two groups were noted in any previously considered clinical outcomes, including hypoxemia, acidemia, or shock. However, shorter duration of apnea testing was observed in the artificial CO
augmentation method suggesting its feasibility to the standard apneic oxygenation method in terms of convenience and reduction of the risk surrounding prolonged hypercapnia and hypoxemia.
This retrospective cohort study provides further observational evidence to support the safety of alternate apnea testing techniques with the standard apnea method for determination of brain death in adults.
This retrospective cohort study provides further observational evidence to support the safety of alternate apnea testing techniques with the standard apnea method for determination of brain death in adults.Hybridisation is still a widely studied phenomenon that allows us to look at some processes differently. However, obtaining fertile hybrids, which we might consider in the long term as precursors of a new species, is still a field that requires research. Much of the research done so far indicates that hybrids are sterile - either sex or both. It is influenced by various mechanisms, both prezygotic and postzygotic reproductive isolation. Thanks to the use of light and transmission electron microscopy, our research has made it possible to understand, at least partially, the causes of infertility in male hybrids resulting from the crossing of two Afrotropical species of the genus Platymeris Laporte, 1833. The analysis of microscopic images showed that one of the possible causes of the infertility of hybrids might be spermatogenesis, during which sperm cells were not formed. In turn, the use of scanning electron microscopy revealed potential abnormalities in the structure of the hybrid eggshell. Moreover, karyotyping analyses suggest possible causes of infertility at the genetic level.
Pediatric chronic rhinosinusitis (PCRS) is a unique clinical entity and the underlying source of inflammation is unknown. Certain subgroups, such as children with nasal polyps and cystic fibrosis (CF) sinusitis are often recalcitrant to standard medical PCRS treatments that target bacterial inflammation. Fungal infection and allergy to fungal proteins drive inflammation in other airway diseases, resulting in chronic inflammation of both the upper and lower airways. However, there is limited understanding of the role of fungi in the pathophysiology of PCRS. The objective of this study is to define the frequency of fungal infection in pediatric CRS patients, hypothesizing that certain subgroups may have more frequent positive fungal sinus cultures than other subgroups of pediatric sinusitis.
Retrospective study of patients undergoing sinus surgery at a tertiary care pediatric hospital to determine the period prevalence of positive fungal cultures in subgroups of patients.
400 children from 2012 to 2019 wepulations to study the impact of fungal infection in CRS. Further research is required to determine if these fungi represent colonization or contribute to the inflammatory environment of the airways.
Leptin, which was only discovered in humans in 1994, has recently been shown to have a possible link with premature ejaculation.
To evaluate whether serum leptin levels differed between patients with premature ejaculation and healthy men, and to analyze the changes in leptin levels before and after treatment.
Six studies assessing the relationship between leptin and premature ejaculation published up to October 2021 were identified from multiple databases (PubMed, Web of Science, Cochrane) and the data were analyzed by Stata software.
Differences in leptin levels in premature ejaculation patients and healthy people, and changes of leptin levels in premature ejaculation population before and after treatment.
Analysis of studies assessing differences in leptin concentrations between patients with PE and healthy men showed that there was a statistically significant difference in leptin levels between PE patients and controls (WMD (95% CI)=17.89 (8.64, 27.14), P < .001). On the other hand, the analysis of data from 3 studies describing serum leptin levels in PE patients before and after treatment with selective serotonin reuptake inhibitors (SSRIs) showed that there was a significant decrease with leptin levels in PE patients after treatment (WMD (95%CI)=22.
