Vindingguy6679

Technologies based on lateral flow immunoassay (LFIA), known in some countries of the world as immunochromatographic tests, have been successfully used for the last six decades in diagnostics of many diseases and conditions as they allow rapid detection of molecular ligands in biosubstrates. The popularity of these diagnostic platforms is constantly increasing in healthcare facilities, particularly those facing limited budgets and time, as well as in household use for individual health monitoring. The advantages of these low-cost devices over modern laboratory-based analyzers come from their availability, opportunity of rapid detection, and ease of use. The attractiveness of these portable diagnostic tools is associated primarily with their high analytical sensitivity and specificity, as well as with the easy visual readout of results. These qualities explain the growing popularity of LFIA in developing countries, when applied at small hospitals, in emergency situations where screening and monitoring health condition is crucially important, and as well as for self-testing of patients. These tools have passed the test of time, and now LFIA test systems are fully consistent with the world's modern concept of 'point-of-care testing', finding a wide range of applications not only in human medicine, but also in ecology, veterinary medicine, and agriculture. The extensive opportunities provided by LFIA contribute to the continuous development and improvement of this technology and to the creation of new-generation formats. This review will highlight the modern principles of design of the most widely used formats of test-systems for clinical laboratory diagnostics, summarize the main advantages and disadvantages of the method, as well as the current achievements and prospects of the LFIA technology. The latest innovations are aimed at improving the analytical performance of LFIA platforms for the diagnosis of bacterial and viral infections, including COVID-19.

species cause a wide spectrum of disease entities.

and

-members of

complex-are currently gaining both clinical and epidemiologic significance.

Totally, 150 pediatric isolates that had previously been identified as

species complex based on a positive germ tube test were included. The isolates were cultured on CHROMagar

medium to ensure their purity and the results of germ tube test. For definitive speciation, PCR amplification and size polymorphism of the

(

) gene was used. The results of

-PCR were confirmed by sequencing the amplified fragments for randomly selected isolates of

and

.

All 150 isolates included in this study were reconfirmed as

complex on chromogenic media. Based on the

gene size polymorphism, 141 (94%) isolates were identified as

, 2 (1.33%) as

, and 1 (0.67%) as

. The remaining 6 (4%)

complex isolates were a mix of

and

. All isolates of

and

have been recovered from cases of candiduria.

, either alone or mixed with

, could be a cause of candiduria among pediatric patients and should not be ignored.

C. africana, either alone or mixed with C. albicans, could be a cause of candiduria among pediatric patients and should not be ignored.The study was conducted at green house and laboratories of Agriculture Botany Department, Faculty of Agriculture, Suez Canal University, Ismailia governorate, Egypt during 2018/2019 to test rhizosphere growth promoting bacteria as known strategy to increase salinity tolerance of six genotypes of wheat namely; Line 404, Line 356, Line 420, Line432, Sakha 93 and Line 380 were grown under 3000 ppm and 5000 ppm of salinity. Four bacterial strains were used namely; Pseudomonas fluorescens NBRC 14160, Serratia liquefaciens ATCC 27592, Bacillus subtilis SBMP4 and Bacillus megaterium NBRC 15308. All the strains could be able to tolerate salinity levels up to 3% NaCl and produced indole acetic acid (IAA). The both strains Pseudomonas fluorescens NBRC 14160 and Bacillus megaterium NBRC 15308 were grow on NA media supplemented with 6% NaCl, and showed 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity and Pseudomonas fluorescens NBRC 14160 strain also fixed nitrogen. PCR results confirmed the previous results for both strains. Pseudomonas fluorescens NBRC 14160 and Bacillus megaterium NBRC 15308 were selected to study their reflection in vivo on wheat plants growth at different levels of salinity. The selected strains were able to improve plants growth under salinity stress conditions when compared with non-inoculated plants for all wheat genotypes especially sakha93 showed the highest mean values over rest genotypes under saline and non-saline conditions. Results of genetic parameters for studied traits showed that values of PCV were higher than GCV values for most studied traits. Germination percentage, shoot length and potassium content had high values of heritability and genetic advance, so these traits might use in selection of plant breeding programs for salinity tolerance.The genus Aspergillus contains diverse species and the identification is complicated. Vegetative compatibility groups (VCGs) and molecular mechanisms were deployed to study the species. The study was randomly conducted in four counties in Kenya based on the history of aflatoxicosis and maize cultivation. Thirty-seven Aspergillus flavus isolates from Nandi, Kisumu, Homa Bay and Makueni were characterized to determine their taxonomic status based on their VCGs and genotypes. A phylogenetic analysis of ITS1 and ITS2 sequences of the isolates investigated revealed ITS primers discriminating some of the A. flavus isolates as 100% sequence identity to the RefSeq. Nit mutants' complementation test revealed strong heterokaryon incompatibility between isolates of Nandi region (67%) and Makueni (33%). The trend based on VCGs and molecular findings showed high incidence of toxigenic A. read more flavus in Makueni, which could be the reason why the region frequently experiences chronic aflatoxicosis incidences over the last few decades as compared to other regions. Interestingly, we have discovered all S and L-morphotypes including the rare S/L-morphotypes, which implies that Kenya is home to all morphotypes of A. flavus. Thus, the analysis provides a deeper understanding of the taxonomic relationship between the A. flavus isolates and could help contextualise the data obtained for each isolate with respect to VCG genetic diversity and genotypes. Determining the primary causal agents of aflatoxin contamination is critical for predicting risk of contamination events and designing and implementing effective management strategies.