Brogaardbredahl1680

We identified an association between the rs10946398 genotypes of CDKAL1 and NAFLD and the TyG index, and the TyG index was related to the risk of NAFLD.

We identified an association between the rs10946398 genotypes of CDKAL1 and NAFLD and the TyG index, and the TyG index was related to the risk of NAFLD.Ocular parasitic infections, which lead to significant morbidity and mortality in particular areas, have been shifting from endemic areas to other regions due to tourism, wars and migrations in recent years. This study aimed to review the parasitic factors related to the human eye and their geographical distribution, pathogenesis of the disease and the methods used in and studies conducted for its diagnosis. PubMed, MEDLINE and Google databases were researched and reviewed for relevant keywords in recent publications. Although such parasitic infections are rare in many parts of the world, they continue to be an important public health problem that affects human and animal health in places with poor health conditions. The distribution of ocular parasites and their spread to non-endemic areas are facilitated. The morbidity and mortality risks have been increasing due to the difficulties encountered by health personnel in the diagnosis of these parasitic infections. Defining them accurately and appropriately can save not only eyesight but also lives.

Microsporidia are opportunistic obligate intracellular pathogens which infect many vertebrate and invertebrate hosts. This study aimed at investigating all evidence about microsporidia infection in human and other vertebrate hosts in Turkey.

This study covered all prevalence studies, related to microsporidiosis in Turkey until April 2020, that were found in Web of Science, PubMed, Scopus, and ULAKBIM databases were considered in this meta-analysis. A total of 168 studies were identified in the systematic literature research. After the initial assessment, only 15 articles (12 humans and three other vertebrates) were included for meta-analysis. Data analysis was carried out using the Revman 5.3 (Review Manage 5.3) software.

With the evaluation of these studies, it was found that the prevalence of microsporidia in humans (n=6.707) and other vertebrate hosts (n=506) was 13.4% and 15.2%, respectively. The risk ratio in the patient groups was 2.87 compared to the control group [95% confidence interval (CI) 1.20-6.87, I2=87%, p<0.00001]. There was no difference between genders and parasite prevalence (95% CI 1.00-1.39, I2=18%, p=0.29). The prevalence of microsporidia was also found to be high in patients with diarrhea (95% CI 1.09-1.58, I2=86%, p=0.0001) and in immunosuppressed individuals (95% CI 1.86-3.70, I2=16%, p=0.31).

Although there are few studies on the prevalence of these parasites, the results of this meta-analysis provides extensive information about the current situation in Turkey.

Although there are few studies on the prevalence of these parasites, the results of this meta-analysis provides extensive information about the current situation in Turkey.

is a protozoan parasite that causes many deaths worldwide. It's cultivation in an in vitro culture setting contributes significantly to scientific studies. However, there are no laboratories in Turkey that cultivate

. Hence, the purpose of this study was to cultivate

.

Five

strains were used in our study and were kept frozen in liquid nitrogen tanks. These parasite strains were then thawed in a 37 °C water bath and transferred to the Albumax-complete medium that was previously prepared. After that, the petri dishes were placed in the chamber. For 30 seconds, a special gas mixture containing 5% CO

, 5% O

and 90% N

was added into the chamber which was placed in a 37 °C oven and left for incubation for 2 days. At the end of the incubation period, thin smear preparations were prepared from the medium, stained with Giemsa and examined using an immersion lens.

Examination of the smears revealed that trophozoite and schizont forms of all

isolates were present at a rate of 2% in

culture medium.

As a result of our study, the

culture of

was successfully developed. With this, several projects such as biological and chemical characteristics, pathogenicity, phenotypic and molecular-level drug sensitivities and parasite vaccination studies can be carried out more easily in our country.

As a result of our study, the in vitro culture of P. falciparum was successfully developed. With this, several projects such as biological and chemical characteristics, pathogenicity, phenotypic and molecular-level drug sensitivities and parasite vaccination studies can be carried out more easily in our country.

Since the identification of

subtypes (ST) in the last decade, much has been learned about the genetic diversity of

isolates in different populations, except pregnant women. The objective of this study is to investigate the genetic diversity of

in pregnant women and analyse some demographic factors.

The faecal samples from 100 pregnant women were collected at an Obstetrics and Gynecology Department in Muğla, Turkey. Idarubicin Thereafter,

positivity was detected by direct microscopy and culture. The positive cultures were subjected to DNA isolation, and the

barcode region was amplified with polymerase chain reaction. Next, the sequences were queried against GenBank nucleotide and

STs (18S) databases.

was detected in 14% (14 out of 100) of the faecal samples by culture and 10% (10 out of 100) of the samples by direct microscopy. Nine of

isolates (64.4%) were ST3, three (21.4%) were ST1 and two (14.2%) were ST2. Neither the demographic features nor the gastrointestinal symptoms were statistically related to

infection.

The findings in this study agreed with the most of the previous human studies that found ST3 as the most abundant genotype. This study reported the frequency of

in pregnant women and highlighted the importance of comprehensive studies with more cases of

during pregnancy.

The findings in this study agreed with the most of the previous human studies that found ST3 as the most abundant genotype. This study reported the frequency of Blastocystis in pregnant women and highlighted the importance of comprehensive studies with more cases of Blastocystis during pregnancy.