Hurstrao4735

However, MDA (kidney), MDA (erythrocyte), (GSH) (erythrocyte), MDA (liver), GSH (liver), and GST (liver) in group V (at 48%, 35%, 34%, 15%, 30%, and 41%, respectively) were significantly lower than in group II. Hence, while L. deliciosus may exhibit tissue-protective against CCl4-induced OS in rats, A. cylindracea does not.The present study aimed to evaluate the antibacterial activity (ABA) of new mushroom strains collected from the mountain and plain forests of Georgia and belonging to different taxonomic groups. Of 30 Basidiomycetes strains tested on agar plates, Schizophyllum commune BCC64 exhibited the highest inhibitory activity against Escherichia coli and Staphylococcus aureus by the diameter of inhibition zones (17 ± 1 mm and 19 ± 1 mm, respectively). Moreover, this mushroom showed strong activity against Staphylococcus enteritidis (11 mm), Pseudomonas aeruginosa (19 mm), and Salmonella epidermitidis (12 mm). In the submerged cultivation in synthetic medium, xylose and glucose ensured the highest ABA toward S. aureus (70% inhibition in microplate rider tests) and E. coli (60%), respectively. Among lignocellulosic materials tested in the submerged and solid-state fermentation, mandarin marc was found to be an excellent growth substrate for ABA accumulation by Sch. commune 64. Of six nitrogen sources, KNO3 favored the mushroom ABA increase against both bacteria. The suitability of the developed nutrient medium has been proven in 7 L fermenter. After fermentation, ethyl acetate extract obtained from culture liquid and ethanol extract obtained from mycelial biomass of Sch. commune 64 showed the best minimum inhibitory concentration (MIC) against E. coli (0.5 and 2.5 mg/mL, respectively) and S. aureus (1 mg/mL for both extracts).Oyster mushrooms (Pleurotus spp.) constitute a substantial resource for functional food, as they are commercially cultivated worldwide and assumed to contain beneficial fiber. The objective of this study was to characterize and analyze the mycelia of Pleurotus ostreatus cv. Florida for its nutrient composition, total dietary fiber, insoluble dietary fiber, soluble dietary fiber content, and fermentability in vitro. Findings showed the mycelia had a moisture content of 18%, ash 12%, protein 5%, fat 0.1%, and carbohydrates 61%. Results also revealed that mycelia are an excellent source of dietary fiber (55%) containing high amounts of insoluble (22%) and soluble (33%) fiber. The mycelia also produced significant amounts of short chain fatty acids after fermentation at in vitro simulating conditions in the colon acetate (1.92 mg/g), propionate (0.89 mg/g), and butyrate (0.31 mg/g). These results indicated that the mycelia of P. ostreatus cv. Florida are promising sources of dietary fiber.Oxidative stress is strongly implicated in aging and in the progression of diseases. Antioxidants, especially of natural origin, are valued for their protective as well as curative health benefits. Numerous mushroom species have shown marked antioxidant effect. This can prove beneficial when mushrooms are used as nutraceuticals or for drug development. In the current investigation the antioxidant potential of some medicinal mushrooms, namely Inonotus pachyphloeus, Phellinus allardii, Ph. fastuosus, Ph. sanfordii and Ph. torulosus was examined employing different assays. The ethanol extracts of the fruiting bodies of these mushrooms were evaluated in vitro for scavenging potential against DPPH, hydroxyl radicals, superoxide radicals, as well as the reducing power. The free radical scavenging activity of mushroom extracts followed the trend of Ph. fastuosus > Ph. sanfordii > Inonotus pachyphloeus > Ph. torulosus > Ph. allardii. click here Ph. fastuosus and Ph. sanfordii extracts exhibited significant DPPH and superoxide radical scavenging activities, statistically (P less then 0.05) comparable to each other and to the standard catechin. Ph. fastuosus extract (EC50 = 16 ± 1.15 μg/mL) showed the most significant hydroxyl radical scavenging activity even higher than the standard. In reducing power assay, Ph. torulosus extract (EC50 = 320 ± 0.02 μg/mL) exhibited the most significant reducing power statistically (P less then 0.05) comparable with the reduced form of glutathione. The tested mushroom extracts were found to consist of appreciable amounts of carbohydrates, phenols and proteins. The free radical scavenging efficacy of the examined mushrooms showed positive correlation with their phenol content. These medicinal mushrooms are good natural antioxidants and can be incorporated in nutraceuticals/pharmaceuticals after detailed studies.Ganoderma lucidum is one of the most famous mushrooms in traditional Chinese medicine. At present, the fully utilized parts of G. lucidum are mainly fruiting body and spore powder. The wild and artificially cultivated G. lucidum fruiting body is costly and rare. Therefore, how to improve the utilization of G. lucidum by means of fermentation is worth investigating. The present study was to perform submerged fermentation of G. lucidum and compare the bioactivities of G. lucidum submerged fermentation broth and fruiting body extract. After the extraction and submerged fermentation methods were optimized, the optimum conditions for extraction were determined as ethanol extraction at 80°C with a solid-to-liquid ratio of 130, and those for submerged fermentation were cultivation on malt extract medium for 6 days at 30°C. Under the optimum conditions, the antioxidative activity and tyrosinase inhibition rate of the fermentation broth were 1.2-4.1 fold higher than those of the ethanol extract. Cytotoxicity analysis showed that the ethanol and water extracts and the fermentation broth effectively inhibited pancreatic cancer cells and prostate cancer cells, with much smaller effect on nontumor human embryonic kidney (HEK293T). These results demonstrate that the submerged fermentation could improve the utilization value of G. lucidum and the fermentation broth can be used as an antioxidant additive applied in food, drugs, and cosmetics.Taiwanofungus camphoratus, a medicinal mushroom indigenous to Taiwan, has attracted the attention of pharmaceutical companies due to its remarkable properties and considerable commercial value. Since it grows slowly and most of its components are chemically unstable, its production and distribution have been problematic. In the present study, cordycipitoid fungi powders of seven species were used to cultivate T. camphoratus, and optimal conditions for biomass production were determined by response surface methodology. The initial liquid medium was enriched with cordycipitoid fungi powders, inoculated with a spore suspension of T. camphoratus, and then incubated on a rotary shaker (120 r/min at 27°C) for 14 days. The effects of cordycipitoid fungi powders on T. camphoratus production were investigated by examining the biomass production of T. camphoratus. The key factors influencing biomass production, as identified by a two-level Plackett-Burman design with eight variables, were (1) powder content of cordycipitoid fungi, (2) glucose content, and (3) bottling volume.