Clemmensensteffensen1405

Dystrophin is a large protein serving as local scaffolding repetitively bridging cytoskeleton and the outside of striated muscle cell. As such dystrophin is a critical brick primarily in dystrophin-associated protein complex (DAGC) and in a larger submembranous unit, costamere. Accordingly, the lack of functional dystrophin laying at the root of Duchenne muscular dystrophy (DMD) drives sarcolemma instability. From this point on, the cascade inevitably leading to the death of myocyte begins. In cardiomyocytes, intracellular calcium overload and related mitochondrial-mediated cell death mainly contribute to myocardial dysfunction and dilation while other protein dysregulation and/or mislocalization may affect electrical conduction system and favor arrhythmogenesis. Although clinically DMD manifests as progressive muscle weakness and skeletal muscle symptoms define characteristic of DMD, it is the heart problem the biggest challenge that most often develop in the form of dilated cardiomyopathy (DCM). Current stahts into basic science, translational efforts and clinical manifestation of dystrophic heart disease.Potato common scab caused by Streptomyces scabies is one of the most economically important diseases infecting potato. It reduces the quality of potato tubers, which subsequently decreases the tuber prices and causes significant economic losses for potato growers. Biological control using bacteriophages is a promising strategy for controlling this disease. In this study, a novel bacteriophage with high lytic efficacy against S. scabies was isolated from a potato field at El-Minya, Egypt, and was designated SscP1EGY. The phage has an icosahedral head of 55 nm and a short tail of 7.5 nm, typical of a podovirus. Its infection cycle was 90 min, including 50 min of latent time and 40 min of rise period with a burst size of approximately 200 PFU per infected cell. The genome of SscP1EGY consists 51,751 nucleotides with 76 predicted genes. SscP1EGY infected and completely lysed seven tested S. scabies strains but showed no lytic activity against three beneficial Streptomyces species, other beneficial bacterial species, and non-target plant pathogenic bacteria. In greenhouse experiments, treatment of S. Bcl2 inhibitor scabies-inoculated potato tubers with phage SscP1EGY resulted in reductions of (1) the severity of scab, (2) the number of lesions, and (3) the percentage of lesion surface, as compared to the inoculated tubers without phage treatment. Also, scab lesions appeared superficial in phage-treated tubers but pitted in non-phage-treated tubers. Our results suggest that SscP1EGY has a potential as a biological control agent for S. scabies. Based on our knowledge, SscP1EGY is the first sequenced S. scabies-infecting phage in Egypt.This study evaluated the anatomical factors that influence the virtual planning of zygomatic implants by using cone beam computerized tomography (CBCT) scans. CBCT scans of 268 maxilla edentulous patients were transferred to a specialized implant planning software (Galaxis, Sirona) for the following measurements maxillo-sinus concavity size (small, medium, and large), Zygoma width, implant insertion angle, implant length, and implant apical anchorage. Concavity sizes found were as follows 34.95% small, 52.30% medium, and 7.35% large. The mean insertion angle was 43.2 degrees and the average implant apical anchorage was 9.1mm. The most frequent implant length was 40mm. Significant differences were found when the different types of concavities in relation to the installation angle, the distance of the apical portion of the implant in contact with the zygomatic bone and the lateral-lateral thickness of the zygomatic bone were compared (p less then 0.001). Medium-sized maxillary sinus concavity presented greater apical anchorage of the implant (9.7mm) and was the most frequent type (52.30%). The zygomatic bone is a viable site for zygomatic fixtures and the use of specialized implant planning software is an important tool to achieve predictable outcomes for zygomatic implants and allows good visualization of the implant-anatomical structures relation.Orphan Implants are encountered when the dentist places an implant, and for some reason, the patient is lost to follow up, and he visits a second dentist for the restoration. The latter will have no clue regarding the implant in the patient's mouth in such a situation. This situation is also expected when the clinician uses multiple implant brands, and the patient reports after some years for a restoration, which leaves the clinician clueless if the clinician does not maintain proper implant records.Extraction of the natural tooth may be a prelude to implant placement. This may be done using an immediate placement protocol or require a delayed approach depending on multiple factors that include; residual infection related to the failed tooth being extracted, availability of bone to stabilize the implant at placement or soft tissue issues. Socket preservation is recommended when the delayed approach is selected to create an osseous bed that can accommodate the implant that is planned. This also helps preserve what crestal bone remains following the extraction that may resorb in the absence of socket preservation during the extraction socket healing phase. The use of osseous graft materials and guided bone-regeneration has demonstrated enhancement of socket healing by potentially modifying the resorption process, yielding preservation of the crestal bone while limiting resorption potential during healing. Various graft materials have been reported including, allografts, xenografts, non-biologics and synthetics. Calcium sulfate as a graft material has been used for many decades in maxillofacial surgeries, plastic surgery, oncologic and orthopedics in the treatment of osseous voids, traumatic or inflammatory bone deficiencies. This article will review a case using biphasic calcium sulfate with a delayed implant protocol and the histology demonstrating conversion of the graft material to host bone following healing.The purpose of this study was to compare heat and sound generated during implant osteotomy when different types of drill were used in artificial bone and bovine bone blocks. A total of 80 implant osteotomies were formed using 4 implant drilling systems; N1 (OsseoShaper) (Nobel), NobelActive (Nobel), V3 (MIS) and BLX (Straumann) in both artificial bone and bovine bone blocks. Thermocouple probes were used to record temperature change at the depths of 5.0 mm and 13.0 mm of each implant osteotomy formed by the final drill. In addition, thermographic images, drilling sound, and drilling time were recorded and evaluated. Statistical analyses were performed at α = 0.05. The mean temperature changes as recorded by thermocouple probes and thermocamera were significantly lower in OsseoShaper than most other drill-bone combinations (p less then .05). The mean drilling times and sound generation for OsseoShaper were significantly higher and lower than most other drill-bone combinations (p less then .05), respectively. Minimal heat and sound generation can be expected when implant osteotomies are performed using Osseoshaper at a low rotational speed (50 rpm) even without irrigation. However, extended drilling time is required.This is an in silico study aimed to evaluate the biomechanical influence of different implant-abutment interfaces (external hexagon and Morse taper implants), retention systems (cement- and screw-retained), and restorative crowns (metal-ceramic and monolithic) using three-dimensional finite element analysis (3D-FEA). Eight 3D models were simulated for the maxillary first molar area using InVesalius, Rhinoceros, and SolidWorks and processed using the Femap and NEi Nastran softwares. Axial and oblique forces of 200 N and 100 N, respectively, were applied on the occlusal surface of the prostheses. Microstrain and von Mises stress maps were used to evaluate the deformation (cortical bone tissue) and stress (implants/fixation screws/crowns), respectively for each model. For both loadings, Morse taper implants had lower microstrain values than the external hexagon implants. The retention system did not affect microstrain on the cortical bone tissue under both loadings. However, the cemented prosthesis displayed higher stress with the fixation screw than the external hexagon implants. No difference was observed between the metal-ceramic and zirconia monolithic crowns in terms of microstrain and stress distribution on the cortical bone, implants or components. Morse taper implants can be considered as a good alternative for dental implant rehabilitation because they demonstrated better biomechanical behavior for the bone and fixation screw as compared to external hexagon implants. Cement-retained prosthesis increased the stress on the fixation screw of the external hexagon implants, thereby increasing the risk of screw loosening/fracture in the posterior maxillary area. The use of metal-ceramic or monolithic crowns did not affect the biomechanical behavior of the evaluated structures.To report the infected silicone chin implant due to the non-ideal placement of dental implants, in female patient, 67 years old. A patient unsatisfied with her facial profile had installed a silicone chin implant 25 years ago. Recently underwent surgery for the placement of dental implants in a region close to the silicone implant. The non-ideal positioning of the dental implants and close contact with the silicone implant led to the absence of osseointegration, with consequent mobility and infection of both. The silicone implant was removed with dental implant that shows mobility. In the imaging exams could be noted others complications of silicone implant like bone resorption and the formation of a narrow bone layer around the inferior border of implant. Alloplastic implants are an option for the esthetic correction of chin deformities. Solid silicone is biocompatible and highly resistant to degradation, with a minimal allergic reaction and risk of toxicity. However, a number of postoperative complications may arise, such as migration or displacement, extrusion, foreign body reaction, bone resorption, heterotopic bone formation and infection. The precise imaging exams are critical to arrive at a diagnosis and the best treatment plan.Sinus floor elevation is the most commonly used method for vertical bone augmentation in the maxillary posterior area. This clinical report describes a modified transalveolar approach to elevate the sinus floor when placing implants on a severely resorbed maxillary posterior ridge with a buccal-palatal distance of more than 8.0 mm. In this approach, the osteotomy prepared on the crestal is bilaterally enlarged to 8.0-10.0 mm. The enlarged osteotomies can provide better access for practitioners, so that instruments can touch directly to the sinus floor and the Schneiderian membrane can be elevated with a reduced risk of perforation.

The use of RBC lysate (RBC-Lys) eliminates the need for serum folate and hematocrit (Hct) measurement to calculate RBC folate. Information on the long-term frozen storage stability of RBC-Lys is missing.

We aimed to assess the comparability of RBC folate forms in whole-blood lysate (WB-Lys) and RBC-Lys and the folate stability in both matrices.

We prepared conventional WB-Lys (111 dilution with 1% ascorbic acid) and RBC-Lys (111 dilution of washed and saline-diluted RBCs with 1% ascorbic acid) from EDTA blood (n=60 adult donors) and stored lysates at -70°C until analysis at baseline (1 wk), 3, 6, 12, and 24mo. Before analysis by HPLC-tandem MS, we incubated the WB-Lys (4h at 37°C) and treated the RBC-Lys with human recombinant γ-glutamyl hydrolase for folate polyglutamate deconjugation. We analyzed RBC-Lys samples for hemoglobin (Hb) (same aliquot) to normalize for the preanalytical dilution; Hb-folate was converted to RBC folate for each folate form using the mean corpuscular Hb concentration. We analyzed Hct as well as folate forms in matching serum samples for traditional RBC folate calculation.