Sanfordborg1601
2 + 3.1% and 65.0 + 4.5% with and without agitation, respectively). Moreover, instar III stage was the most sensitive to PS-NH2 exposure (38.2% immobility in 24 h of exposure; 5 μg mL-1). Evidence of PS-NH2 retention in the gastrointestinal tract in a concentration- and time-dependent manner was also obtained. Mixtures of PS-NH2 (0.005 and 5 μg mL-1) with different concentrations of K2Cr2O7 increased the immobilization rate of the larvae after 48 h of exposure, when compared to the K2Cr2O7 alone. Similar results were observed for CuSO4 in the co-exposure conditions at different concentrations. However, exposing nauplii to a mixture of PS-NH2 (0.005 μg mL-1) and CuSO4 decreased immobilization rate, in comparison to the group exposed to CuSO4 alone. The present work highlights the potential risk posed by nanoplastics to zooplanktonic species through their interaction with other toxicants.The present study empirically investigates the tripartite impacts of renewable energy (RE), nonrenewable energy (NRE), and trade openness (TO) with the conditioning role of technology on environmental quality (CO2 emission) for the G-7 countries (Canada, France, Germany, Japan, Italy, USA, and United Kingdom) for the period straddling 1990-2019. The empirical analyses are anchored on a set of estimation procedures including; cross-sectional dependence test, second generation panel unit root test, Westerlund cointegration test, Hausman test, and pooled mean group (PMG). The following results emanate from the findings. First, the presence of cross-sectional dependence and long-run relationships are confirmed for the countries. Second, RE significantly lessens the prevalence of carbon emissions across the estimated models. This further underscores the mitigating effects of RE on CO2 emissions for the G-7 countries. Third, the impacts of NRE and TO are found to contribute to surge in CO2 emissions. Fourth, the effects of technological progress captured by research and development (RD) and eco-innovation significantly reduce the stock of CO2 emissions using both unconditional (single effect) and conditional (interactive effect) methods. Fifth, the existence of Environmental Kuznets Curve (EKC) receives empirical support for the G-7 countries. Other covariates such as foreign direct investment (FDI), Gross Fixed Capital Formation (GCFC), and service value-added (SVA) exert diverging impacts on CO2 emissions. Sixth, the country-level analyses show the heterogeneous nature of the G-7 countries as evident from each country's findings.Copper oxychloride gained great importance due to its broad-spectrum antifungal action to combat various fungal diseases of plants. However, excess quantity of cupric fungicides on plants causes enzymatic changes and toxic effects. Thus, the current study was aimed to investigate the cytotoxicity and genotoxicity of copper oxychloride on Allium cepa root cells. The root growth, mitotic index (MI), chromosomal aberrations (CAs), and DNA damage were assessed through root growth inhibition, A. cepa ana-telophase, and alkaline comet assays. Furthermore, molecular docking was performed to evaluate binding affinities of two copper oxychloride polymorphs (atacamite and paratacamite) on DNA. Reversine Adenosine Receptor antagonist In root growth inhibition test, onion root length was statistically significantly decreased by changing the copper oxychloride concentration from lower (2.64±0.11 cm) to higher (0.92±0.12 cm). Concentration- and time-dependent decrease in MI was observed whereas increase in CAs such as disturbed ana-telophase, chromosome laggards, stickiness, anaphase bridges, and DNA damage were caused by the copper oxychloride on A. cepa root cells. Molecular docking results revealed that the two main polymorphs of copper oxychloride (atacamite and paratacamite) bind selectively to G and C nucleotides on the B-DNA structure. It is concluded that the atacamite- and paratacamite-induced DNA damage may be through minor groove recognition and intercalation. Findings of the current study revealed the cytotoxic and genotoxic effects of copper oxychloride on A. cepa root cells. However, further studies should be carried out at the molecular level to reveal the cyto-genotoxic mechanism of action of copper oxychloride in detail.Cardboard factory wastewater is usually known by high chemical oxygen demand (COD), color, phenols, lignin, and its derivatives, and usual treatment techniques are not able to treat such wastewaters. This study aimed to investigate the efficiency of ozone-assisted electrocoagulation process (EC/O3) for the treatment of real cardboard wastewater. The parameters influencing COD removal in the EC/O3 process were optimized using response surface methodology. Regard to the statistical model, the optimum conditions were obtained at current density 9.6 mA/cm2, time 20 min, and pH 12. At optimal condition, EC/O3 process removed 74.7% and 97.5% of COD and color, which was higher compared to ozonation and EC processes separately. The COD removal followed pseudo-first-order kinetic with the coefficient correlation of 0.97 and the reaction rate constant of 0.073 1/min. To sum up, the combined electrocoagulation process with ozonation could be used satisfactorily for removing pollutants from real cardboard wastewater.Remediation of soil contaminated with cadmium (Cd) that can produce rice enriched with selenium (Se) is highly significant for improving the public health in China. A key issue needing resolution in this regard is the simultaneous immobilization of Cd and mobilization of Se. To explore a potential promising method to remediate median-high Se soil that is contaminated by Cd, a potted experiment was conducted, and seven combined amendments (0.03-0.12% lime, 0.03-0.18% bentonite, and 0.3-1.2% biochar of the dry soil weight) were used to immobilize Cd in three paddy soils, in which the concentrations of Cd and Se are 0.46 and 0.45 mg/kg, 1.12 and 0.33 mg/kg, and 2.96 and 0.31 mg/kg, respectively. The soil pH increased by 1.5-2 units after the application of the amendments, and the soil organic carbon (SOC) concentration increased notably with the addition of large quantities of biochar. As the pH and SOC concentration increased, the concentrations of the available Cd in the soil decreased by 35-50%, and the FTIR spectrum showed that O-containing groups and Si-O facilitated the Cd immobilization. The concentration of Cd in brown rice decreased with a decrease in the available Cd. There was no apparent correlation between the Se concentration in the brown rice and the concentration of the available Se in the soil, although the available Se increased by 40-80% after the application of amendments. The accumulation of Se in rice grains was regulated by interactions among the lime, bentonite, biochar, and the soil. An SEM-EDS analysis showed that the biochar particles were covered with bentonite and other soil minerals that could postpone biochar aging and contribute to the longevity of the combined amendments in the soil.The microbiome is a community of various microorganisms that inhabit or live on the skin of humans/animals, sharing the body space with their hosts. It is a sort of complex ecosystem of trillions of commensals, symbiotic, and pathogenic microorganisms, including trillions of bacteria, archaea, protozoa, fungi, and viruses. The microbiota plays a role in the health and disease status of the host. Their number, species dominance, and viability are dynamic. Their long-term disturbance is usually accompanied by serious diseases such as metabolic disorders, cardiovascular diseases, or even cancer. While epigenetics is a term that refers to different stimuli that induce modifications in gene expression patterns without structural changes in the inherited DNA sequence, these changes can be reversible or even persist for several generations. Epigenetics can be described as cell memory that stores experience against internal and external factors. Results from multiple institutions have contributed to the role and close interaction of both microbiota and epigenetics in disease induction. Understanding the mechanisms of both players enables a better understanding of disease induction and development and also opens the horizon to revolutionary therapeutic approaches. The present review illustrates the roles of diet, microbiome, and epigenetics in the induction of several chronic diseases. In addition, it discusses the application of epigenetic data to develop diagnostic biomarkers and therapeutics and evaluate their safety for patients. Understanding the interaction among all these elements enables the development of innovative preventive/therapeutic approaches for disease control.The establishment of landfill covers consumes substantial amounts of fuels and materials that contribute to greenhouse gas emissions and fossil fuel depletion (FFD). This study compared the global warming impact (GWI) and FFD of a shrub willow evapotranspiration (ET), conventional clay, and geosynthetic cover using life cycle assessment (LCA). The willow ET cover is a carbon-negative system with a total GWI of -13,206 kgCO2eq ha-1 over 30 years, while the GWI of clay (194,916 kgCO2eq ha-1) and geosynthetic covers (260,212 kgCO2eq ha-1) were substantial. Willow's ability to store carbon in coarse roots and stools creates the opportunity for negative carbon emissions. The FFD impact of the willow ET cover (75,303 MJ surplus ha-1) is 4.7 times lower than the clay cover and 7.7 times lower than the geosynthetic cover. Sensitivity and uncertainty analyses indicated that the GWI of the willow ET cover was less than zero for all scenarios, and the GWI and FFD of the geosynthetic cover were the highest. Willow ET covers can protect human health and the environment, reduce greenhouse gas emissions by sequestering carbon, and provide a source of wood chips that could be used for renewable energy or other applications.Organophosphate (OP) pesticides may accumulate in pregnant agricultural workers, resulting in adverse effects on the growth and development of the fetus and neonates. This study aims to evaluate a possible association between prenatal urinary OP metabolite levels among pregnant agricultural workers and birth outcomes of infants. This study also investigated the factors associated with urinary OP metabolites among pregnant agricultural workers. The spot urine samples were collected and analyzed for six OP metabolite levels. Birth outcomes data were abstracted from medical records. Multiple regression analysis found that gestational age at childbirth was negatively associated with diethylphosphate (DEP) levels (β = -0.073; 95% CI, -0.121, -0.024). Apgar score at 1 and 5 min after birth were negatively associated with diethyldithiophosphate (DEDTP) levels (β = -0.036; 95% CI, -0.069, -0.003; and β = -0.034, 95% CI, -0.057, -0.011, respectively). In addition, DEDTP levels were negatively associated with maternal age (β = -0.181; 95% CI, -0.339, -0.023), and dimethylphosphate (DMP) levels were positively associated with frequency of agricultural work during pregnancy (β = 31.554; 95% CI, 0.194, 62.914). Our results indicate that prenatal OP exposure can cause adverse birth outcomes in babies. Therefore, it is necessary to develop an effective strategy for reducing prenatal exposure to OP pesticides.